Search Results - "Rúa, M.L."

An esterase from Thermus thermophilus HB27 with hyper-thermoalkalophilic properties: Purification, characterisation and structural modelling by Fuciños, P., Pastrana, L., Sanromán, A., Longo, M.A., Hermoso, J.A., Rúa, M.L.

“…[Display omitted] ► A membrane-associated esterase (E34Tt) was detected in Thermus thermophilus HB27. ► E34Tt was purified, and identified by peptide mass…”
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Purification and characterization of Lip2 and Lip3 isoenzymes from a Candida rugosa pilot-plant scale fed-batch fermentation by Pernas, M.A., López, C., Pastrana, L., Rúa, M.L.

“…Previous purification of a crude extracellular enzyme preparation from Candida rugosa ATCC 14830 pilot-plant fed-batch fermentations showed the presence of two…”
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Use of Poly(N-isopropylacrylamide) Nanohydrogels for the Controlled Release of Pimaricin in Active Packaging by Fuciños, C., Guerra, N.P., Teijón, J.M., Pastrana, L.M., Rúa, M.L., Katime, I.

“…:  We propose here a delivery drug‐polymer system using poly(N‐isopropylacrylamide) (PNIPA) nanohydrogels that enables pimaricin to be protected from hostile…”
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Antimicrobial effect of pimaricin-loaded thermosensitive nanohydrogels by Fuciños, C., Fajardo, P., Amado, I.R., Guerra, N.P., Pastrana, L.M., Rúa, M.L.

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Identification of extracellular lipases/esterases produced by Thermus thermophilus HB27: Partial purification and preliminary biochemical characterisation by Fuciños, P., Abadín, C.M., Sanromán, A., Longo, M.A., Pastrana, L., Rúa, M.L.

“…Thermus thermophilus HB27 produces important levels of extracellular lipolytic activity when grown for 30 h at 70 °C in a complex medium. A method to detect…”
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Production and characterization of two heterologous esterases from Thermus thermophilus in a mesophilic yeast by Solaroli, M., Fuciños, P., Atanes, E., Siso, M.I., Pastrana, L., Rúa, M.L.

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Reactivity of Pure Candida rugosa Lipase Isoenzymes (Lip1, Lip2, and Lip3) in Aqueous and Organic Media. Influence of the Isoenzymatic Profile on the Lipase Performance in Organic Media by López, Neus, Pernas, María A., Pastrana, Lorenzo M., Sánchez, Antoni, Valero, Francisco, Rúa, María L.

“…Three pure isoenzymes from Candida rugosa lipase (CRL: Lip1, Lip2, and Lip3) were compared in terms of their stability and reactivity in both aqueous and…”
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Lipolytic enzyme production by Thermus thermophilus HB27 in a stirred tank bioreactor by Dominguez, Alberto, Pastrana, Lorenzo, Longo, Maria A., Rúa, Maria L., Sanroman, M. Angeles

“…In this study, lipolytic enzyme production by Thermus thermophilus HB27 at bioreactor scale has been investigated. Cultivation was performed in a 5-L stirred…”
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Production of Thermostable Lipolytic Activity by Thermus Species by Fuciños, Pablo, Domínguez, Alberto, Angeles Sanromán, M., Longo, María A., Luisa Rúa, M., Pastrana, Lorenzo

“…A quantitative screening for intra‐ and extracellular lipolytic activity was performed in submerged cultures of four Thermus strains using two different media…”
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A controlled fed-batch cultivation for the production of new crude lipases from Candida rugosa with improved properties in fine chemistry by Sánchez, A, Ferrer, P, Serrano, A, Valero, F, Solà, C, Pernas, M, Rúa, M.L, Fernández-Lafuente, R, Guisán, J.M, de la Casa, R.M, Sinisterra, J.V, Sánchez-Montero, J.M

“…A controlled constant feeding rate fed-batch strategy using oleic acid as inducer produced a crude lipase preparation from Candida rugosa (CRL-UAB) with higher…”
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Quantification of intra- and extra-cellular thermophilic lipase/esterase production by Thermus sp by DOMINGUEZ, A, SANROMAN, A, FUCINOS, P, RUA, M. L, PASTRANA, L, LONGO, M. A

“…Four Thermus strains produced lipolytic activity when grown in liquid medium for 30 h at 70 degrees C. The highest total lipase/esterase activity (57 U l(-1))…”
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Structural basis for the kinetics of Candida rugosa Lip1 and Lip3 isoenzymes by Pernas, M, López, C, Prada, A, Hermoso, J, Rúa, M.L

“…The fungus Candida rugosa produces several lipase isoenzymes and the 3D structure of two were solved (Lip1 and Lip3). We have isolated homodimers of Lip3…”
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Purification and characterization of two isoforms from Candida rugosa lipase B by LOPEZ, Cristina, GUERRA, Nelson P, RUA, M. Luisa

“…Two isoforms of Candida rugosalipase B (LB1 and LB2) were purified by anionic exchange chromatography. The lipases had the same N-terminal sequence,…”
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Thermoalkalophilic lipase of Bacillus thermocatenulatus: Large-scale production, purification and properties: aggregation behaviour and its effect on activity by Luisa Rúa, M, Schmidt-Dannert, Claudia, Wahl, Sabine, Sprauer, Achim, Schmid, Rolf D

“…Escherichia coli BL321 was transformed with the expression plasmid pCYTEXP1 carrying the BTL2 gene from Bacillus thermocatenulatus under the control of the…”
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PCN38 Cost-Effectiveness of Mammography As a Screening Test for EARLY Detection of Breast Cancer by Jervis-Jalabe, D.S., Rua-Quintana, M.L., Avendaño, C.C., Beltran-Venegas, T., Gaviria-Garcia, G.G., Alvis-Guzman, N., Orozco Africano, J., Olivares-Cerpa, F.

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Fractionation and characterization of proteins from Gevuina avellana and Rosa rubiginosa seeds by Moure, A, Rua, M.L, Dominguez, H

“…Gevuina avellana and Rosa rubiginosa proteins were evaluated for their potential food use. The proteins were sequentially separated into five fractions…”
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Rapid purification of two lipase isoenzymes from Candida rugosa by Rua, M.L, Ballesteros, A

“…We describe a two-step method for the purification of two lipases (lipases A and B) from C. rugosa. The purification procedure includes Phenyl-Sepharose and…”
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