Search Results - "Rúa, M. Luisa"

Influence of pH on viscoelastic properties of heat-induced gels obtained with a β-Lactoglobulin fraction isolated from bovine milk whey hydrolysates by Estévez, Natalia, Fuciños, Pablo, Bargiela, Verónica, Picó, Guillermo, Valetti, Nadia Woitovich, Tovar, Clara Asunción, Rúa, M. Luisa

“…•β-Lg fraction (r-βLg) was recovered from whey hydrolysates obtained with cardosins.•Structural and rheological properties of r-βLg were studied at different…”
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Structural and thermo-rheological analysis of solutions and gels of a β-lactoglobulin fraction isolated from bovine whey by Estévez, Natalia, Fuciños, Pablo, Bargiela, Verónica, Pastrana, Lorenzo, Tovar, Clara Asunción, Luisa Rúa, M.

“…•A β-Lg fraction was recovered from whey hydrolysates obtained with cardosins.•Changes in the structure of β-Lg were detected by fluorescence spectroscopy.•At…”
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Modeling the angiotensin-converting enzyme inhibitory activity of peptide mixtures obtained from cheese whey hydrolysates using concentration-response curves by Estévez, Natalia, Fuciños, Pablo, Sobrosa, Ana C., Pastrana, Lorenzo, Pérez, Nelson, Luisa Rúa, M.

“…Three mathematical models, two logistic models (previously published in previous works) and one mechanistic, developed in this work and based on…”
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Production and characterization of two N-terminal truncated esterases from Thermus thermophilus HB27 in a mesophilic yeast: Effect of N-terminus in thermal activity and stability by Fuciños, Pablo, Atanes, Estrella, López-López, Olalla, Esperanza Cerdán, M., Isabel González-Siso, M., Pastrana, Lorenzo, Luisa Rúa, M.

“…Two N-terminally truncated variants of the esterase E34Tt from Thermus thermophilus HB27 (YP_004875.1) were expressed in Kluyveromyces lactis. Production and…”
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Production of Thermostable Lipolytic Activity by Thermus Species by Fuciños, Pablo, Domínguez, Alberto, Angeles Sanromán, M., Longo, María A., Luisa Rúa, M., Pastrana, Lorenzo

“…A quantitative screening for intra‐ and extracellular lipolytic activity was performed in submerged cultures of four Thermus strains using two different media…”
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Effectiveness of proteolytic enzymes to remove gluten residues and feasibility of incorporating them into cleaning products for industrial purposes by Fuciños, Clara, Estévez, Natalia, Míguez, Martín, Fajardo, Paula, Chapela, María José, Gondar, Dora, Rúa, M. Luisa

“…The development of protocols for efficient gluten elimination is one of the most critical aspects of any allergen management strategy in the industry. The…”
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Cloning, expression, purification and characterization of an oligomeric His-tagged thermophilic esterase from Thermus thermophilus HB27 by Fuciños, Pablo, Atanes, Estrella, López-López, Olalla, Solaroli, Michele, Cerdán, M. Esperanza, González-Siso, M. Isabel, Pastrana, Lorenzo, Rúa, M. Luisa

“…•A thermophilic membrane-associated esterase from Thermus thermophilus HB27 was expressed in Escherichia coli.•E34Tt-His6 was purified from the E. coli…”
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Heterologous expression of an esterase from Thermus thermophilus HB27 in Saccharomyces cerevisiae by López-López, Olalla, Fuciños, Pablo, Pastrana, Lorenzo, Rúa, M. Luisa, Cerdán, M. Esperanza, González-Siso, M. Isabel

“…In this work, a system for high-level secretion by Saccharomyces cerevisiae of the Thermus thermophilus HB27 putative esterase YP_004875.1 was constructed. The…”
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Thermal spring water enhances lipolytic activity in Thermus thermophilus HB27 by Fuciños, Pablo, Rúa, M. Luisa, Longo, María A., Sanromán, M. Ángeles, Pastrana, Lorenzo

“…Spring thermal water from different origin and mineral composition was evaluated to prepare the culture media for Thermus thermophilus HB27. The growth and…”
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Hydrolysis of whey protein as a useful approach to obtain bioactive peptides and a β-Lg fraction with different biotechnological applications by Estévez, Natalia, Fuciños, Pablo, Fuciños, Clara, Jauregi, Paula, Tovar, Clara A., Rúa, M. Luisa

“…Most of the studies related to the susceptibility of the main bovine whey proteins to the action of cardosins of Cynara cardunculus have underestimated the…”
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Stimulation of novel thermostable extracellular lipolytic enzyme in cultures of Thermus sp by Domínguez, Alberto, Fuciños, Pablo, Rúa, M. Luisa, Pastrana, Lorenzo, Longo, María A., Sanromán, M. Angeles

“…Selected organisms from the genus Thermus ( T. aquaticus YT1, T. thermophilus HB8 and HB27) offer new opportunities for biocatalysis and biotransformations as…”
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Thermoalkalophilic lipase of Bacillus thermocatenulatus: Large-scale production, purification and properties: aggregation behaviour and its effect on activity by Luisa Rúa, M, Schmidt-Dannert, Claudia, Wahl, Sabine, Sprauer, Achim, Schmid, Rolf D

“…Escherichia coli BL321 was transformed with the expression plasmid pCYTEXP1 carrying the BTL2 gene from Bacillus thermocatenulatus under the control of the…”
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Two novel lipases from thermophile Bacillus thermocatenulatus: screening, purification, cloning, overexpression, and properties by Schmidt-Dannert, C, Rúa, M L, Schmid, R D

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Heptyl oleate synthesis as useful tool to discriminate between lipases, proteases and other hydrolases in crude preparations by Domı́nguez de Marı́a, Pablo, Martı́nez-Alzamora, Fernando, Moreno, Sergio Pérez, Valero, Francisco, Rúa, M.Luisa, Sánchez-Montero, José M, Sinisterra, José V, Alcántara, Andrés R

“…Heptyl oleate synthesis is a good test to compare crude lipases. Being a reaction selective for lipases, esterases and proteases are not active in this…”
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Purification and characterization of two isoforms from Candida rugosa lipase B by LOPEZ, Cristina, GUERRA, Nelson P, RUA, M. Luisa

“…Two isoforms of Candida rugosalipase B (LB1 and LB2) were purified by anionic exchange chromatography. The lipases had the same N-terminal sequence,…”
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Regulation of the interfacial activation within the Candida rugosa lipase family by Pernas, María A., Pastrana, Lorenzo, Fuciños, Pablo, Rúa, M. Luisa

“…The fungus Candida rugosa produces several lipase isoenzymes and the 3D structure of three was solved (Lip1, Lip2 and Lip3). In an aqueous solution, the…”
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Thermoalkalophilic lipase of Bacillus thermocatenulatus by Luisa Rúa, M, Schmidt-Dannert, Claudia, Wahl, Sabine, Sprauer, Achim, Schmid, Rolf D

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Structural Insights into the Lipase/esterase Behavior in the Candida rugosa Lipases Family: Crystal Structure of the Lipase 2 Isoenzyme at 1.97 Å Resolution by Mancheño, José M., Pernas, Marı́a A., Martı́nez, Marı́a J., Ochoa, Begoña, Rúa, M.Luisa, Hermoso, Juan A.

“…The yeast Candida rugosa produces several closely related extracellular lipases that differ in their substrate specificity. Here, we report the crystal…”
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Influence of the conformational flexibility on the kinetics and dimerisation process of two Candida rugosa lipase isoenzymes by Pernas, Marı́a A., López, Cristina, Rúa, M.Luisa, Hermoso, Juan

“…We have investigated the interfacial activation process of two isoenzymes from Candida rugosa (Lip1 and Lip3) using triacetin as substrate. Kinetics were…”
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Structural insights into the lipase/esterase behavior in the Candida rugosa lipases family: crystal structure of the lipase 2 isoenzyme at 1.97A resolution by Mancheño, José M, Pernas, María A, Martínez, María J, Ochoa, Begoña, Rúa, M Luisa, Hermoso, Juan A

“…The yeast Candida rugosa produces several closely related extracellular lipases that differ in their substrate specificity. Here, we report the crystal…”
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