Alternative splicing of human cyclin E

Alternative splicing of human cyclin E

Cyclin E is a regulatory subunit of the cdc2-related protein kinase cdk2, which is activated shortly before S-phase entry, thus defining it as a G1 cyclin. We report here the existence of a 43 kDa splice variant of human cyclin E, termed cyclin Es, which lacks 49 amino acids within the cyclin box co...

Full description

Saved in:
Bibliographic Details
Published in:Journal of cell science Vol. 107; no. 2; pp. 581 - 588
Main Authors: SEWING, A, RÖNICKE, W, BÜRGER, C, FUNK, M, MÜLLER, R
Format: Journal Article
Language:English
Published: Cambridge Company of Biologists 01-02-1994
Subjects:
Alternative Splicing
Amino Acid Sequence
Base Sequence
Biological and medical sciences
CDC2-CDC28 Kinases
Cell cycle, cell proliferation
Cell Line
Cell physiology
Cyclin-Dependent Kinase 2
Cyclin-Dependent Kinases
Cyclins - genetics
Cyclins - metabolism
DNA Primers - genetics
Fundamental and applied biological sciences. Psychology
Humans
Molecular and cellular biology
Molecular Sequence Data
Mutation
Protein Kinases - metabolism
Protein Serine-Threonine Kinases
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
RNA, Messenger - genetics
RNA, Messenger - metabolism
Saccharomyces cerevisiae - genetics
Proteins
Human
Alternative splicing
Regulation(control)
Molecular processing
Messenger RNA
Enzyme
Cell cycle
Cyclin
Gene expression
Subunit
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cyclin E is a regulatory subunit of the cdc2-related protein kinase cdk2, which is activated shortly before S-phase entry, thus defining it as a G1 cyclin. We report here the existence of a 43 kDa splice variant of human cyclin E, termed cyclin Es, which lacks 49 amino acids within the cyclin box compared to the known 48 kDa cyclin E. Cyclin Es is expressed at approximately 1/10 of the level of full-length cyclin E in several cell lines analysed. The two cyclin E forms differ functionally in that cyclin E, but not cyclin Es, is able to complex with cdk2, to activate the histone H1, pRb and p107 in vitro kinase activity of cdk2 and to rescue a triple CLN mutation in S. cerevisiae. Cyclin Es is the first splice variant of a cell cycle regulatory protein to be described. Our findings also indicate that the cyclin box in cyclin E mediates the interaction with cdk2.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
ISSN:0021-9533
1477-9137
DOI:10.1242/jcs.107.2.581