Effects of mannoprotein E1 in liquid diet on inflammatory response and TLR5 expression in the gut of rats infected by Salmonella typhimurium

Mannoproteins are yeast cell wall componend, and rich in mannose. The use of foods rich in mannose as carbohydrate, could have a bioprotective effect against entrobacteria intestinal infection. Nothing is known about mannoproteins' activity in inflammatory bowel processes induced by entrobacter...

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Published in:	BMC gastroenterology Vol. 10; no. 1; p. 58
Main Authors:	Posadas, Sinforiano J, Caz, Victor, Caballero, Isabel, Cendejas, Emilio, Quilez, Immaculada, Largo, Carlota, Elvira, Marcos, De Miguel, Enrique
Format:	Journal Article
Language:	English
Published:	England BioMed Central Ltd 08-06-2010 BioMed Central BMC
Subjects:	Administration, Oral Animal experimentation Animals Apoptosis Cell Proliferation Cytokines - metabolism Disease Models, Animal Dose-Response Relationship, Drug Down-Regulation Gene expression Inflammation - drug therapy Inflammation - metabolism Inflammation - prevention & control Intestines - metabolism Intestines - microbiology Intestines - pathology Male Membrane Glycoproteins - administration & dosage Membrane Glycoproteins - therapeutic use Physiological aspects Proteins Rats Rats, Wistar Salmonella Salmonella Infections - metabolism Salmonella Infections - pathology Salmonella typhimurium Toll-Like Receptor 5 - metabolism
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Summary:	Mannoproteins are yeast cell wall componend, and rich in mannose. The use of foods rich in mannose as carbohydrate, could have a bioprotective effect against entrobacteria intestinal infection. Nothing is known about mannoproteins' activity in inflammatory bowel processes induced by entrobacteria.This study investigates the effects of mannoprotein administration via a liquid diet on inflammatory response and TLR5 expression during intestinal tissue injury in a rat model of infection with Salmonella typhimurium. Adult Wistar male rats were divided into three groups: control, and mannoprotein E1 at 10 or 15%. Animals were fed with a liquid diet supplemented or not with mannoprotein E1. Groups were infected by intragastrical administration of S. typhimurium. 24 h post-inoculation samples of spleen, ileum and liver were collected for microbiological studies. Gut samples were processed to determine levels of proinflammatory cytokines (mRNA) and TLR5 (mRNA and protein) by quantitative PCR and Western-blot, and the number of proliferative and apoptotic cells determined by immunohistochemistry. Ininfected levels of proinflammatory cytokines and TLR5 were higher in untreated controls than in the animals receiving mannoprotein. Proliferation was similar in both groups, whereas apoptosis was higher in controls. Curiosly, the mannoprotein effect was dose dependent. Mannoprotein administration in a liquid diet seems to protect intestinal tissue against S. typhimurium infection. This protection seems to expressed as a lower pro-inflammatory response and TLR5 downregulation in gut epithelium, as well as by an inhibition of apoptosis. Nevertheless, the molecular mechanism by which mannoprotein is able to regulate these responses remain unclear. These results could open up new avenues in the use of mannoproteins as prebiotics in the therapeutic strategy for treatment of inflammatory gut processes induced by microbia.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1471-230X 1471-230X
DOI:	10.1186/1471-230X-10-58