Bioassay-guided isolation of α-glucosidase inhibitors from Byrsonima garcibarrigae Cuatrec

Bioassay-guided isolation of α-glucosidase inhibitors from Byrsonima garcibarrigae Cuatrec

Introduction:Byrsonima garcibarrigae is an endemic tree of Amazonas state, Brazil, with pharmacological and chemical knowledge poorly understood. Aim: To investigate the antidiabetic potential of the B. garcibarrigae stem bark. Methods: The stem bark was sequentially extracted by maceration with hex...

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Published in:Revista colombiana de ciencias químico-farmacéuticas Vol. 51; no. 3
Main Authors: Marques Machado, Tallita, Queiroz Valentim, Carolina, Domingo Rosales Acho, Leonard, Silva Lima, Emerson, Ferreira Koolen, Hector Henrique, Guilhon-Simplicio, Fernanda
Format: Journal Article
Language:English
Published: Bogota Universidad Nacional de Colombia 2022
Subjects:
Antidiabetics
Antioxidants
Bark
Chloroform
Composting
Ethyl acetate
Fractionation
Glucosidase
Hexanes
NMR
Nuclear magnetic resonance
Oxidative stress
Phenols
Stems
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Summary:Introduction:Byrsonima garcibarrigae is an endemic tree of Amazonas state, Brazil, with pharmacological and chemical knowledge poorly understood. Aim: To investigate the antidiabetic potential of the B. garcibarrigae stem bark. Methods: The stem bark was sequentially extracted by maceration with hexane (EHBG), ethyl acetate (EABG), and methanol (EMBG). The antioxidant capacity, α-glucosidase inhibitory potentials and anti-glycation capacities were evaluated. A bio-guided fractionation gave compounds that were characterized by MS and NMR. Results: 8 compounds were identified by HPLC-MS. EMBG showed the highest α-glucosidase inhibitory activity (1.09±0.32 μg/mL), antioxidant activity (9.2±0.23 μg/mL) and phenolic compounds content (61.43±0.50%), thus was fractionated producing hexane (FHX), chloroform (FCL) and hydromethanolic (FHM) fractions. After additional anti-α-glucosidase assays, FHM (1.02±0.49 μg/mL) was fractionated giving quercitrin and epicatechin. The anti-glycation assay showed that EMBG, FHM and quercitrin presented higher activities in comparison to the positive control, aminoguanidine. Conclusions:B. garcibarrigae displayed antidiabetic potential since inhibited α-glucosidase, as well as presented expressive antioxidant and anti-glycation activities were recorded.
ISSN:0034-7418
1909-6356
DOI:10.15446/rcciquifa.v51n3.96982