An in-tube aptamer/gold nanoparticles coated capillary solid-phase microextraction for separation of adenosine in serum and urine samples
•An aptamer/AuNPs coated in-tube solid-phase microextraction capillary was prepared.•2.84 μg cm−1 of the bonding amount of Au coated on the inner surface was obtained.•2.44 μg of aptamer immobilization capacity was obtained in a 27-cm capillary.•Aptamer/AuNPs coated capillary showed improved selecti...
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| Published in: | Journal of Chromatography A Vol. 1611; p. 460617 |
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| Main Authors: | , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Netherlands
Elsevier B.V
25-01-2020
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | •An aptamer/AuNPs coated in-tube solid-phase microextraction capillary was prepared.•2.84 μg cm−1 of the bonding amount of Au coated on the inner surface was obtained.•2.44 μg of aptamer immobilization capacity was obtained in a 27-cm capillary.•Aptamer/AuNPs coated capillary showed improved selectivities to adenosine.•Adenosine was monitored with aptamer/AuNPs coated capillary SPME-HPLC-UV method.
As an endogenous nucleoside, adenosine was significant for the diagnosis and treatment of some diseases, such as schizophrenia. However, due to the complicated matrix interference, it was difficult to monitor trace or ultra-trace adenosine directly in bio-samples. In this contribution, a novel in-tube SPME technique based on aptamer/Au nanoparticles coated open tubular fused-silica capillary was established to separate and enrich adenosine in bio-samples with high affinity. Therefore, a uniform and dense AuNPs layer was coated on the inner surface of the open tubular capillary, and then adenosine aptamer was immobilized on AuNPs with a high capacity of 2.44 μg per 27-cm capillary. As a result, the capillary shown high selectivity to adenosine with a selectivity factor of 14.4 when compared with the scrambled aptamer/AuNPs coated capillary. Also, the extraction amount of adenosine was 2.8–24.8 times higher than those of its structural analogs and contrast, such as guanosine, uridine, cytidine, thymidine, and toluic acid. After the optimization of extraction conditions, the aptamer/AuNPs coated in-tube SPME-HPLC method was developed for the adenosine assay with the linear range of 0.002–0.100 μg mL−1 and the detection limit of 0.45 ng mL−1. Subsequently, the approach was applied for trace adenosine monitoring in human serum and urine samples. It showed a strong performance of reducing matrix interference and improving sensitivity, and the spiking recoveries of 89.9–92.6% and 91.1–94.5% were achieved respectively. |
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| ISSN: | 0021-9673 1873-3778 |
| DOI: | 10.1016/j.chroma.2019.460617 |