Transcriptomic analysis unravels the molecular response of Lonicera japonica leaves to chilling stress
is not only an important resource of traditional Chinese medicine, but also has very high horticultural value. Studies have been performed on the physiological responses of leaves to chilling, however, the molecular mechanism underlying the low temperature-induced leaves morphological changes remain...
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| Published in: | Frontiers in plant science Vol. 13; p. 1092857 |
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| Main Authors: | , , , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Switzerland
Frontiers Media S.A
22-12-2022
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | is not only an important resource of traditional Chinese medicine, but also has very high horticultural value. Studies have been performed on the physiological responses of
leaves to chilling, however, the molecular mechanism underlying the low temperature-induced leaves morphological changes remains unclear. In this study, it has been demonstrated that the ratio of pigments content including anthocyanins, chlorophylls, and carotenoids was significantly altered in response to chilling condition, resulting in the color transformation of leaves from green to purple. Transcriptomic analysis showed there were 10,329 differentially expressed genes (DEGs) co-expressed during chilling stress. DEGs were mainly mapped to secondary metabolism, cell wall, and minor carbohydrate. The upregulated genes (UGs) were mainly enriched in protein metabolism, transport, and signaling, while UGs in secondary metabolism were mainly involved in phenylpropaoids-flavonoids pathway (PFP) and carotenoids pathway (CP). Protein-protein interaction analysis illustrated that 21 interacted genes including
,
,
, and
were enriched in calcium transport/potassium ion transport. BR biosynthesis pathway related genes and
(BRI) were collectively induced by chilling stress. Furthermore, the expression of genes involved in anthocyanins and CPs as well as the content of chlorogenic acid (CGA) and luteoloside were increased in leaves of
under stress. Taken together, these results indicate that the activation of PFP and CP in leaves of
under chilling stress, largely attributed to the elevation of calcium homeostasis and stimulation of BR signaling, which then regulated the PFP/CP related transcription factors. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Sue Lin, Wenzhou University, China; Ping Luo, Zhejiang Agriculture and Forestry University, China These authors have contributed equally to this work and share first authorship Edited by: Mudassar Nawaz Khan, Hazara University, Pakistan This article was submitted to Technical Advances in Plant Science, a section of the journal Frontiers in Plant Science |
| ISSN: | 1664-462X 1664-462X |
| DOI: | 10.3389/fpls.2022.1092857 |