PreS deletion mutations of hepatitis B virus in chronically infected patients with simultaneous seropositivity for hepatitis-B surface antigen and anti-HBS antibodies

Hepatitis B surface antigen (HBsAg) and anti-HBs antibodies (anti-HBs) may coexist in certain chronic hepatitis B (CHB) patients. This study was designed to further explore the relationship between this coexistence and hepatitis B Virus (HBV) preS deletions. Sera of 28 patients carrying both HBsAg a...

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Published in:	Journal of medical virology Vol. 82; no. 1; pp. 23 - 31
Main Authors:	Huang, Xiangyan, Qin, Yanghua, Zhang, Peng, Tang, Gusheng, Shi, Qingfen, Xu, Jun, Qi, Falian, Shen, Qian
Format:	Journal Article
Language:	English
Published:	Hoboken Wiley Subscription Services, Inc., A Wiley Company 2010 Wiley
Subjects:	Amino Acid Sequence anti-HBs Base Sequence Biological and medical sciences deletion mutant Fundamental and applied biological sciences. Psychology Genotype HBsAg Hepatitis B Antibodies - blood Hepatitis B Surface Antigens - blood Hepatitis B Surface Antigens - chemistry Hepatitis B Surface Antigens - genetics Hepatitis B virus - genetics Hepatitis B virus - immunology Hepatitis B, Chronic - immunology Hepatitis B, Chronic - virology Human viral diseases Humans Infectious diseases Medical sciences Microbiology Miscellaneous Molecular Sequence Data preS1 preS2 Protein Precursors - chemistry Protein Precursors - genetics Sequence Analysis, DNA Sequence Deletion Viral diseases Virology Human anti-HBs Seropositivity deletion mutant Hepatitis B surface antigen Antibody Orthohepadnavirus Hepatic disease HBsAg Infection Virus Viral hepatitis B Chronic Hepadnaviridae Viral disease Deletion Digestive diseases Mutation Hepatitis B virus preS2 preS1
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Summary:	Hepatitis B surface antigen (HBsAg) and anti-HBs antibodies (anti-HBs) may coexist in certain chronic hepatitis B (CHB) patients. This study was designed to further explore the relationship between this coexistence and hepatitis B Virus (HBV) preS deletions. Sera of 28 patients carrying both HBsAg and anti-HBs (Group I) and those of another 28 HBsAg positive but anti-HBs negative patients (Group II) were collected from CHB patients. Direct sequencing of polymerase chain reaction products or sequencing of clones was applied to both groups to determine sequences of HBV preS and S genes. Genotyping of the S gene indicated that all sampled HBVs were either Genosubtype Ba or Genosubtype Ce. Seven samples in Group I harbored HBV preS deletion mutations. Three of the seven samples showed large deletion mutations in 3' terminus of preS1 and co-existence of the mutant type and the full-length wild type, and the remaining four samples showed deletion mutations in 5' terminus of preS2. All mutant strains were found to be genosubtype Ce. Only two samples in Group I showed G145R/A mutation. Only one sample in Group II contained preS deletion mutation. It is therefore concluded that HBV preS deletion mutations are likely to be related to the coexistence of HBsAg and anti-HBs in CHB patients (P-value = 0.024). Some immune reactions may select for the preS deletion in CHB patients with anti-HBs, the possible marker for immune selection. J. Med. Virol. 82:23-31, 2010.
Bibliography:	http://dx.doi.org/10.1002/jmv.21669 ArticleID:JMV21669 All experimental investigations on human subjects mentioned in this article received official institutional and ethical approval from the Ethics Committee of the Second Military Medical University. ark:/67375/WNG-476P4Q9B-H istex:CF66E1828A91D18D91FE71A133363488BFF4916C ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0146-6615 1096-9071
DOI:	10.1002/jmv.21669