PFGE and AFLP genotyping of Staphylococcus aureus subsp. anaerobius isolated from goats with Morel’s disease

PFGE and AFLP genotyping of Staphylococcus aureus subsp. anaerobius isolated from goats with Morel’s disease

Staphylococcus aureus subsp. anaerobius is the etiological agent of the Morel’s disease in sheep and goats. The disease presents with subcutaneous abscesses, located mainly in the superficial lymph nodes. Forty-one isolates of S. aureus subsp. anaerobius were collected from two outbreaks of the More...

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Bibliographic Details
Published in:Archives of microbiology Vol. 195; no. 1; pp. 37 - 41
Main Authors: Szaluś-Jordanow, O., Chrobak, D., Pyrgiel, M., Lutyńska, A., Kaba, J., Czopowicz, M., Witkowski, L., Kizerwetter-Świda, M., Binek, M., Frymus, T.
Format: Journal Article
Language:English
Published: Berlin/Heidelberg Springer-Verlag 01-01-2013
Springer Nature B.V
Subjects:
Abscess - microbiology
Abscess - veterinary
Abscesses
Amplified Fragment Length Polymorphism Analysis
Animal diseases
Animals
Biochemistry
Biomedical and Life Sciences
Biotechnology
Cell Biology
Deoxyribonucleic acid
DNA
Ecology
Electrophoresis, Gel, Pulsed-Field
Genotype
Goat Diseases - microbiology
Goats
Life Sciences
Lymph nodes
Lymphatic system
Microbial Ecology
Microbiology
Original Paper
Pathogens
Phylogeny
Poland
Sheep
Staphylococcal Infections - microbiology
Staphylococcal Infections - veterinary
Staphylococcus aureus - classification
Staphylococcus aureus - genetics
Staphylococcus aureus - isolation & purification
Veterinary medicine
Poland
PFGE
Goat
Morel’s disease
subsp
AFLP
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Summary:Staphylococcus aureus subsp. anaerobius is the etiological agent of the Morel’s disease in sheep and goats. The disease presents with subcutaneous abscesses, located mainly in the superficial lymph nodes. Forty-one isolates of S. aureus subsp. anaerobius were collected from two outbreaks of the Morel’s disease in Poland in years 2006–2008. Analysis of DNA Sma I digests by PFGE showed that 35 of 41 isolates belonged to the same PFGE type, identical to the type strain of S. aureus subsp. anaerobius ATCC 35844, confirming high level of clonality of the species. The DNA patterns of the remaining identical 6 isolates, different from the reference strain only by two bands, were found closely related. Genotyping performed with AFLP technique revealed two clonal groups including 16 and 25 isolates, respectively. The study indicated that AFLP technique might be a better discriminatory tool for genetic analysis of S. aureus subsp. anaerobius isolates, when compared to PFGE.
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content type line 23
Communicated by Erko Stackebrandt.
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-012-0844-8