Search Results - "Pushpalatha P. N. Murthy"
-
1
Development of a Semester-Long, Inquiry-Based Laboratory Course in Upper-Level Biochemistry and Molecular Biology
Published in Journal of chemical education (11-11-2014)“…A semester-long laboratory course was designed and implemented to familiarize students with modern biochemistry and molecular biology techniques. The designed…”
Get full text
Journal Article -
2
Lily pollen alkaline phytase is a histidine phosphatase similar to mammalian multiple inositol polyphosphate phosphatase (MINPP)
Published in Phytochemistry (Oxford) (01-09-2006)“…An alkaline phytase with unique catalytic properties was cloned and characterized from lily pollen. The enzyme is a histidine phytase with low similarity (less…”
Get full text
Journal Article -
3
Enhancement of alkaline phytase production in Pichia pastoris: Influence of gene dosage, sequence optimization and expression temperature
Published in Protein expression and purification (01-08-2012)“…► Recombinant alkaline phytase (rLlALP2) was expressed in Pichia pastoris. ► The effect of copy number, gene optimization and temperature on yield was…”
Get full text
Journal Article -
4
Origin and Seed Phenotype of Maize low Phytic Acid 1-1 and Low Phytic Acid 2-1
Published in Plant physiology (Bethesda) (01-09-2000)“…Phytic acid (myo-inositol-1, 2, 3, 4, 5, 6-hexakisphosphate or Ins P6) typically represents approximately 75% to 80% of maize (Zea mays) seed total P. Here we…”
Get full text
Journal Article -
5
Heterologous expression and functional characterization of a plant alkaline phytase in Pichia pastoris
Published in Protein expression and purification (01-12-2010)“…Phytases catalyze the sequential hydrolysis of phytic acid ( myo-insositol hexakisphosphate), the most abundant inositol phosphate in cells. Phytic acid…”
Get full text
Journal Article -
6
Seed phosphorus and inositol phosphate phenotype of barley low phytic acid genotypes
Published in Phytochemistry (Oxford) (01-03-2003)“…myo-Inositol-1,2,3,4,5,6-hexa kisphosphate (Ins P 6 or “phytic acid”) typically represents ∼75% of the total phosphorus and >80% of soluble myo-inositol (Ins)…”
Get full text
Journal Article -
7
Synergy of Intramolecular Hydrogen-Bonding Network in myo-Inositol 2-Monophosphate: Theoretical Investigations into the Electronic Structure, Proton Transfer, and pKa
Published in Journal of the American Chemical Society (16-11-2005)“…This work demonstrates the pivotal role that an intramolecular hydrogen-bonding network (intra-HBN) plays in the determination of the conformation of…”
Get full text
Journal Article -
8
Influence of Metal Cations on the Intramolecular Hydrogen-Bonding Network and pK a in Phosphorylated Compounds
Published in The journal of physical chemistry. A, Molecules, spectroscopy, kinetics, environment, & general theory (10-05-2007)“…The binding of the most common metal cations of cytoplasm (Li+, Na+, K+, Mg2+ and Ca2+) to a model molecule having an intramolecular hydrogen-bonding network,…”
Get full text
Journal Article -
9
Biosynthesis and localization of phosphatidyl‐scyllo‐inositol in barley aleurone cells
Published in Lipids (01-01-1999)“…A novel isomer of phosphatidylinositol (PI), phosphatidyl‐scyllo‐inositol, was characterized in the aleurone cells of barley seeds. In this investigation, the…”
Get full text
Journal Article -
10
Influence of Metal Cations on the Intramolecular Hydrogen-Bonding Network and pKa in Phosphorylated Compounds
Published in The journal of physical chemistry. A, Molecules, spectroscopy, kinetics, environment, & general theory (10-05-2007)“…The binding of the most common metal cations of cytoplasm (Li+, Na+, K+, Mg2+ and Ca2+) to a model molecule having an intramolecular hydrogen-bonding network,…”
Get full text
Journal Article -
11
Structure and nomenclature of inositol phosphates, phosphoinositides, and glycosylphosphatidylinositols
Published in Sub-cellular biochemistry (2006)Get more information
Journal Article -
12
Evidence of Two Isomers of Phosphatidylinositol in Plant Tissue
Published in Plant physiology (Bethesda) (01-04-1992)“…The structure of phosphatidylinositol in barley (Hordeum vulgare) aleurone layers was investigated by chemical degradation. In vivo myo-[2-3H]inositol-labeled…”
Get full text
Journal Article -
13
Phosphoinositides in barley aleurone layers and gibberellic acid-induced changes in metabolism
Published in Plant physiology (Bethesda) (01-12-1989)“…Phospholipids of barley (Hordeum vulgare L. cv Himalaya) aleurone layers were labeled with myo-[2-3H]inositol or [32Pi], extracted, and analyzed by physical…”
Get full text
Journal Article -
14
A fast and easy technique for the isolation of aleurone layers
Published in Plant physiology (Bethesda) (01-06-1989)“…Although the aleurone layer of cereal grain seeds has many advantages for the study of gibberellin action, it has the disadvantage that the hand-isolation of…”
Get full text
Journal Article -
15
Trichodiene biosynthesis and the enzymic cyclization of farnesyl pyrophosphate
Published in Journal of the American Chemical Society (01-04-1981)Get full text
Journal Article -
16
The isolation and characterization of right-side-out plasma membrane vesicles from barley aleurone cells
Published in Lipids (01-01-1999)“…Examination of organelle‐ and membrane‐specific processes such as signal transduction necessitates the use of plasma membrane vesicles with cytoplasmic side‐in…”
Get full text
Journal Article -
17
Extracellular expression of alkaline phytase in Pichia pastoris: Influence of signal peptides, promoters and growth medium
Published in Biotechnology reports (Amsterdam, Netherlands) (01-06-2015)“…Alkaline phytase isolated from pollen grains of Lilium longiflorum (LlALP) possesses unique catalytic and thermal stability properties that suggest it has the…”
Get full text
Journal Article -
18
-
19
-
20