Search Results - "Pursel, V. G."

Birth of Piglets After Transfer of Embryos Cryopreserved by Cytoskeletal Stabilization and Vitrification by Dobrinsky, J. R, Pursel, V. G, Long, C. R, Johnson, L. A

“…Pig embryos suffer severe sensitivity to hypothermic conditions, which limits their ability to withstand conventional cryopreservation. Research has focused on…”
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Cell Donor Influences Success of Producing Cattle by Somatic Cell Nuclear Transfer by Powell, A. M, Talbot, N. C, Wells, K. D, Kerr, D. E, Pursel, V. G, Wall, R. J

“…To assess sources of variation in nuclear transfer efficiency, bovine fetal fibroblasts (BFF), harvested from six Jersey fetuses, were cultured under various…”
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Glutathione concentration during maturation and after fertilization in pig oocytes: relevance to the ability of oocytes to form male pronucleus by Yoshida, M, Ishigaki, K, Nagai, T, Chikyu, M, Pursel, V.G

“…The present study examined the kinetics of glutathione (GSH) concentration during maturation and after fertilization in pig oocytes and its relevance to the…”
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Total body and regional measurements of bone mineral content and bone mineral density in pigs by dual energy X-ray absorptiometry by Mitchell, A. D, Scholz, A. M, Pursel, V. G

“…Dual energy X-ray absorptiometry (DXA) was used to make total body and regional measurements of bone mineral content, bone mineral density, and bone area…”
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Composition analysis of pork carcasses by dual-energy X-ray absorptiometry by Mitchell, A.D, Scholz, A.M, Pursel, V.G, Evock-Clover, C.M

“…Dual-energy x-ray absorptiometry (DXA) was used as a noninvasive method to measure the composition of pig carcasses. A total of 181 half-carcasses (10 to 51…”
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Prediction of pork carcass composition based on cross-sectional region analysis of dual energy X-ray absorptiometry (DXA) scans by Mitchell, A.D, Scholz, A.M, Pursel, V.G

“…Dual energy X-ray absorptiometry (DXA) was used to measure pork carcass composition by performing a total scan of the right half of 262 pork carcasses…”
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Correlation between histochemically assessed fiber type distribution and isomyosin and myosin heavy chain content in porcine skeletal muscles by Bee, G, Solomon, M.B, Czerwinski, S.M, Long, C, Pursel, V.G

“…Highly sensitive enzyme assays developed to differentiate skeletal muscle fibers allow the recognition of three main fiber types: slow-twitch oxidative (SO),…”
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Status of research with transgenic farm animals by Pursel, V.G, Rexroad, C.E. Jr

“…Microinjection is the predominant method used to transfer genes into farm animals. Current research is devoted to improvement of productivity traits,…”
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Inability of heterologous growth hormone (GH) to regulate GH binding protein in GH-transgenic swine by Cifone, D, Dominici, F. P, Pursel, V. G, Turyn, D

“…Transgenic pigs expressing bovine, ovine, or human growth hormone (GH) structural genes fused to mouse metallothionein-I (mMT-bGH), ovine MT (oMT-oGH), or…”
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High-Level Synthesis of a Heterologous Milk Protein in the Mammary Glands of Transgenic Swine by Wall, Robert J., Pursel, Vernon G., Shamay, Avi, McKnight, Robert A., Pittius, Christoph W., Hennighausen, Lothar

“…The whey acidic protein (WAP) is a major milk protein in mice, rats, and rabbits but has not been found in milk of livestock including swine. To determine…”
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Porcine follicle-stimulating hormone treatment of gilts during an altrenogest-synchronized follicular phase: effects on follicle growth, hormone secretion, ovulation, and fertilization by Guthrie, H.D, Pursel, V.G, Wall, R.J

“…Porcine FSH (SUPER OV), containing .03% LH activity, and equine chorionic gonadotropin (eCG) were administered during an altrenogest-synchronized follicular…”
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Transfer of an ovine metallothionein-ovine growth hormone fusion gene into swine by Pursel, V.G, Wall, R.J, Solomon, M.B, Bolt, D.J, Murray, J.D, Ward, K.A

“…An ovine metallothionein-1a (oMT1a)-ovine growth hormone (oGH) fusion gene was microinjected into 400 pig zygotes, the zygotes were transferred into recipient…”
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Genetic engineering of livestock by Pursel, V.G. (USDA, ARS, Beltsville, MD.), Pinkert, C.A, Miller, K.F, Bolt, D.J, Campbell, R.G, Palmiter, R.D, Brinster, R.L, Hammer, R.E

“…Genetic engineering of livestock is expected to have; major effect on the agricultural industry. However, accurate assessment of the consequences of transgene…”
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Dual-Energy X-ray Absorptiometry Measurements of the Body Composition of Pigs of 90- to 130-Kilograms Body Weight by MITCHELL, A. D., SCHOLZ, A. M., PURSEL, V. G.

“…: We used the pig as an in vivo model for evaluating the effects of extreme body depth on the measurement of body composition by dual‐energy X‐ray…”
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Codon optimization, genetic insulation, and an rtTA reporter improve performance of the tetracycline switch by Wells, K.D, Foster, J.A, Moore, K, Pursel, V.G, Wall, R.J

“…The objective of this work was to further develop a tetracycline repressor (TetR) protein system that allows control of transgene expression. First, to…”
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Identification of pig primordial germ cells by immunocytochemistry and lectin binding by Takagi, Y. (Shinshu University, Nagano, Japan.), Talbot, N.C, Rexroad, C.E. Jr, Pursel, V.G

“…Monoclonal antibodies anti‐SSEA‐1 and EMA‐1, and the lectins DBA and LTA, bound to the surface of large, round cells randomly distributed in the 26‐day pig…”
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Follicular expression of a human beta-cell leukaemia/lymphoma-2 (Bcl-2) transgene does not decrease atresia or increase ovulation rate in swine by Guthrie, H D, Wall, R J, Pursel, V G, Foster-Frey, J A, Donovan, D M, Dawson, H D, Welch, G R, Garrett, W G

“…Transgenic (TG) gilts carrying a human Bcl-2 cDNA transgene driven by mouse inhibin-alpha subunit promoter were produced and evaluated to determine if ectopic…”
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Confocal and fluorescence microscopic study using lectins of the distribution of cortical granules during the maturation and fertilization of pig oocytes by Yoshida, M, Cran, D G, Pursel, V G

“…A study was carried out to determine whether pig cortical granules (CGs) could be visualized using fluorescein isothiocyanate (FITC)-labelled lectins…”
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Development of porcine ova that were centrifuged to permit visualization of pronuclei and nuclei by Wall, R.J, Pursel, V.G, Hammer, R.E, Brinster, R.L

“…The obscured pronuclei or nuclei in living one- and two-celled pig ova were revealed after centrifugation for 3 min at 15,000 X g. To determine viability of…”
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Lipid composition of carcass tissue from transgenic pigs expressing a bovine growth hormone gene by Solomon, M.B, Pursel, V.G, Paroczay, E.W, Bolt, D.J

“…Fatty acid profiles and cholesterol content of whole-carcass ground tissue were compared from 26 transgenic (T) pigs expressing a bovine growth hormone gene…”
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