Search Results - "Puri, N K"

Spectrofluorimetric assessment of the surface hydrophobicity of proteins by CARDOMONE, M, PURI, N. K

“…The equilibrium binding of the apolar fluorescent dye 1-anilinonaphthalene-8-sulphonate (ANS) to bacteriorhodopsin, BSA, chicken egg lysozyme, ovalbumin,…”
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Solubilization of growth hormone and other recombinant proteins from Escherichia coli inclusion bodies by using a cationic surfactant by PURI, N. K, CRIVELLI, E, CARDAMONE, M, FIDDES, R, BERTOLINI, J, NINHAM, B, BRANDON, M. R

“…Recombinant pig growth hormone (rPGH) was solubilized from inclusion bodies by using the cationic surfactant cetyltrimethylammonium chloride (CTAC). The…”
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A relationship between the starting secondary structure of recombinant porcine growth hormone solubilised from inclusion bodies and the yield of native (monomeric) protein after in vitro refolding by Puri, N.K., Cardamone, M.

“…Recombinant porcine growth hormone (rPGH) was solubilised from inclusion bodies (IB's) using either 6 M guanidinium hydrochloride (GnHCl), 7.5 M urca or by a…”
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Sheep lymphocyte antigens (OLA). II: Major histocompatibility complex class II molecules by PURI, N. K, MACKAY, C. R, BRANDON, M. R

“…A panel of monoclonal antibodies have been produced which recognize monomorphic determinants of sheep MHC Class II antigens, including an allogenically derived…”
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N-terminal amino acid sequence analyses of the alpha and beta polypeptides from four distinct subsets of sheep major histocompatibility complex class II molecules by Puri, NK, Walker, ID, Brandon, MR

“…Four monoclonal antibodies, SBU.II 28-1, 37-68, 38-27, and 42-20, each recognizing a distinct, non-overlapping subset of sheep class II molecules, were used to…”
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Sheep MHC class II molecules. II: Identification and characterization of four distinct subsets of sheep MHC class II molecules by PURI, N. K, BRANDON, M. R

“…A panel of seven monoclonal antibodies, sequential immunoprecipitation and two-dimensional NEPHGE/SDS-PAGE analyses were used to identify and characterize…”
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Comparison of conventional adjuvants and 'adjuvant-free' monoclonal antibody targeting for stimulating antibody responses against a conjugate of luteinizing hormone releasing hormone and avidin by Tiong, G K, Gill, H S, Lofthouse, S, Puri, N K

“…A conjugate of luteinizing hormone releasing hormone (LHRH) and avidin was used for immunizing mice and sheep. In mice, a range of adjuvants co-administered…”
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Sheep MHC class II molecules. I: Immunochemical characterization by PURI, N. K, GORRELL, M. D, BRANDON, M. R

“…The physicochemical features, biosynthesis and glycosylation of sheep class II molecules were investigated using a panel of seven monoclonal antibodies. The…”
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Production of methionyl-minus ovine growth hormone in Escherichia coli and one-step purification by Adams, T E, MacIntosh, B, Brandon, M R, Wordsworth, P, Puri, N K

“…The overproduction of ovine growth hormone (oGH) in Escherichia coli is described, achieved in part by alteration of the codon usage for nine of the first 15…”
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Refolding of recombinant porcine growth hormone in a reducing environment limits in vitro aggregate formation by Puri, Nirdosh K.

“…Recombinant porcine growth hormone (rPGH) solubilized from bacterial inclusion bodies (IBs) using a cationic surfactant was oxidized to form disulphide bonds…”
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Signature of triply excited Li-like V states in ion–solid collisions by Sharma, Gaurav, Haris, K., Singh, G., Kumar, B., Karmakar, S., Puri, N.K., Mishra, Adya P., Kumar, Pravin, Nandi, T.

“…The transitions originating from triply excited, doubly autoionizing states of Li-like V formed in beam-foil experiments detected within a set of blended…”
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p-Type gas-sensing behaviour of undoped SnO2 thin films irradiated with a high-energy ion beam by Rani, Sanju, Bhatnagar, M.C., Roy, Somnath C., Puri, N.K., Kanjilal, D.

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A functional and biochemical analysis of bovine class II MHC antigens using monoclonal antibodies by Emery, D L, Puri, N K, Dufty, J H, Gorrell, M D, Brandon, M R

“…Monoclonal antibodies (MAbs) reacting with bovine (2) ovine (3), murine (1) or human (1) Class II MHC antigens were examined for reactivity with bovine…”
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A spectroscopic and equilibrium binding analysis of cationic detergent-protein interactions using soluble and insoluble recombinant porcine growth hormone by Cardamone, M, Puri, N K, Sawyer, W H, Capon, R J, Brandon, M R

“…Overexpression of cloned eukaryote genes in bacteria often leads to the formation of insoluble refractile bodies which require solubilization by harsh…”
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Comparing the Refolding and Reoxidation of Recombinant Porcine Growth Hormone from a Urea Denatured State and from Escherichia coli Inclusion Bodies by Cardamone, Michael, Puri, Nirdosh K, Brandon, Mal R

“…Overexpression of cloned genes in bacteria often leads to insoluble refractile body formation requiring solubilization and refolding to obtain biologically…”
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Effect of swift heavy ion irradiation on structure, optical, and gas sensing properties of SnO2 thin films by Rani, Sanju, Puri, N.K., Roy, Somnath C., Bhatnagar, M.C., Kanjilal, D.

“…Swift heavy ion irradiation has been successfully used to modify the structural, optical, and gas sensing properties of SnO2 thin films. The SnO2 thin films…”
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Biochemical and molecular analysis of sheep MHC class II molecules by Puri, N K, Scott, P C, Choi, C L, Brandon, M R

“…A panel of monoclonal antibodies was used for structural and immunodepletion analysis of sheep MHC class II molecules. The results indicate the antibodies…”
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Identification of cysteine-containing peptides during the peptide mapping of recombinant proteins by Cardamone, M, Ashman, K, Brandon, M R, Puri, N K

“…Confirmation of a protein's cysteine content and of its location within the amino acid sequence is crucial in investigating the structural integrity of…”
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p-Type gas-sensing behaviour of undoped SnO sub(2) thin films irradiated with a high-energy ion beam by Rani, S, Bhatnagar, M C, Roy, S C, Puri, N K, Kanjilal, D

“…We report novel p-type behaviour of undoped SnO sub(2) thin films irradiated with a 75MeV Ni super(+) ion beam. Gas response of the irradiated films to NH…”
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Monoclonal antibodies to sheep MHC class I and class II molecules: biochemical characterization of three class I gene products and four distinct subpopulations of class II molecules by Puri, N K, Gogolin-Ewens, K J, Brandon, M R

“…The availability of a panel of monoclonal antibodies to sheep MHC class I and class II gene products has allowed for the first time an assessment of the…”
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