Specific and common changes in Nicotiana benthamiana gene expression in response to infection by enveloped viruses

1 201F Plant Science Building, Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA 2 Department of Statistics, University of Kentucky, Lexington, KY 40506, USA 3 Advanced Genetics Technologies Center (AGTC), University of Kentucky, Lexington, KY 40546, USA Correspondence...

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Published in:	Journal of general virology Vol. 86; no. 9; pp. 2615 - 2625
Main Authors:	Senthil, G, Liu, H, Puram, V.G, Clark, A, Stromberg, A, Goodin, M.M
Format:	Journal Article
Language:	English
Published:	Reading Soc General Microbiol 01-09-2005 Society for General Microbiology
Subjects:	Biological and medical sciences Fundamental and applied biological sciences. Psychology gene expression Gene Expression Profiling Gene Expression Regulation Genetics host-pathogen relationships Impatiens necrotic spot virus infection messenger RNA microarray technology Microbiology Miscellaneous Nicotiana - genetics Nicotiana - metabolism Nicotiana - virology Nicotiana benthamiana Oligonucleotide Array Sequence Analysis - methods Plant Diseases - virology plant diseases and disorders Plant Leaves - virology Plant Proteins - genetics Plant Proteins - metabolism Rhabdoviridae - pathogenicity Sonchus yellow net virus Tospovirus - pathogenicity transcription (genetics) Transcription, Genetic Virology Microbiology Dicotyledones Angiospermae Spermatophyta Solanaceae Experimental plant Gene expression Virology
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Summary:	1 201F Plant Science Building, Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA 2 Department of Statistics, University of Kentucky, Lexington, KY 40506, USA 3 Advanced Genetics Technologies Center (AGTC), University of Kentucky, Lexington, KY 40546, USA Correspondence M. M. Goodin mgoodin{at}uky.edu Microarrays derived from Solanum tuberosum expressed sequence tags were used to test the hypothesis that genetically distinct enveloped viruses elicit unique changes in Nicotiana benthamiana gene expression. The results of our study, which included Sonchus yellow net virus (SYNV), a plant rhabdovirus that replicates in the nucleus of infected cells, and Impatiens necrotic spot virus (INSV), a plant bunyavirus that replicates in the cytoplasm, were consistent with this hypothesis. Statistically significant changes ( P 0·01) in the expression of 275, 2646 and 4165 genes were detected in response to INSV at 2, 4 and 5 days post-inoculation (d.p.i.), respectively. In contrast, 35, 665 and 1458 genes were expressed differentially in response to SYNV at 5, 11 and 14 d.p.i., respectively. The microarray results were verified by Northern hybridization using a subset of these genes as probes. Notably, INSV, but not SYNV, induced expression of small heat-shock protein genes to high levels. In contrast to SYNV, infection by INSV resulted in downregulation of all histone genes, of which the downregulation of histone 2b expression to very low levels was confirmed by Northern hybridization. The expression of a putative WRKY transcription factor at 11 d.p.i., but not at 5 or 14 d.p.i., in SYNV-infected tissue suggested that the temporal response to virus infection was identified readily using our experimental design. Overall, infection by INSV resulted in larger fold changes in host gene expression relative to infection by SYNV. Taken together, the present data demonstrate differential responses of a common host to two genetically distinct viruses. Raw microarray data and more comprehensive lists of the Gene Ontology results are available as supplementary material in JGV Online.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0022-1317 1465-2099
DOI:	10.1099/vir.0.81043-0