Search Results - "Pupo, Elder"

Activation of Canine, Mouse and Human TLR2 and TLR4 by Inactivated Leptospira Vaccine Strains by Novak, Andreja, Pupo, Elder, Van't Veld, Esther, Rutten, Victor P M G, Broere, Femke, Sloots, Arjen

“…Canine vaccines contain inactivated strains of pathogenic , the causative agents of leptospirosis. For an effective response to vaccination, activation of the…”
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Shortening the Lipid A Acyl Chains of Bordetella pertussis Enables Depletion of Lipopolysaccharide Endotoxic Activity by Arenas, Jesús, Pupo, Elder, Phielix, Coen, David, Dionne, Zariri, Afshin, Zamyatina, Alla, Tommassen, Jan, van der Ley, Peter

“…Whooping cough, or pertussis, is an acute respiratory infectious disease caused by the Gram-negative bacterium Whole-cell vaccines, which were introduced in…”
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Naturally circulating pertactin-deficient Bordetella pertussis strains induce distinct gene expression and inflammatory signatures in human dendritic cells by Kroes, Michiel M., Miranda-Bedate, Alberto, Hovingh, Elise S., Jacobi, Ronald, Schot, Corrie, Pupo, Elder, Raeven, René H. M., van der Ark, Arno A. J., van Putten, Jos P. M., de Wit, Jelle, Mariman, Rob, Pinelli, Elena

“…Respiratory infections caused by Bordetella pertussis are reemerging despite high pertussis vaccination coverage. Since the introduction of the acellular…”
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Vaccine-Mediated Activation of Human TLR4 Is Affected by Modulation of Culture Conditions during Whole-Cell Pertussis Vaccine Preparation by Hoonakker, Marieke E, Verhagen, Lisa M, Pupo, Elder, de Haan, Alex, Metz, Bernard, Hendriksen, Coenraad F M, Han, Wanda G H, Sloots, Arjen

“…The potency of whole-cell pertussis (wP) vaccines is still determined by an intracerebral mouse protection test. To allow development of suitable in vitro…”
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Nanoflow LC–MS Method Allowing In-Depth Characterization of Natural Heterogeneity of Complex Bacterial Lipopolysaccharides by Pupo, Elder, van der Ley, Peter, Meiring, Hugo D

“…The variable modification of the outer membrane lipopolysaccharide (LPS) in Gram-negative bacteria contributes to bacterial pathogenesis through various…”
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Lipid A heterogeneity and its role in the host interactions with pathogenic and commensal bacteria by Saha, Sukumar, Pupo, Elder, Zariri, Afshin, van der Ley, Peter

“…Lipopolysaccharide (LPS) is for most but not all Gram-negative bacteria an essential component of the outer leaflet of the outer membrane. LPS contributes to…”
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Substrate specificity of the pyrophosphohydrolase LpxH determines the asymmetry of Bordetella pertussis lipid A by Arenas, Jesús, Pupo, Elder, de Jonge, Eline, Pérez-Ortega, Jesús, Schaarschmidt, Joerg, van der Ley, Peter, Tommassen, Jan

“…Lipopolysaccharides are anchored to the outer membrane of Gram-negative bacteria by a hydrophobic moiety known as lipid A, which potently activates the host…”
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Modulating endotoxin activity by combinatorial bioengineering of meningococcal lipopolysaccharide by Zariri, Afshin, Pupo, Elder, van Riet, Elly, van Putten, Jos P. M., van der Ley, Peter

“…Neisseria meningitidis contains a very potent hexa-acylated LPS that is too toxic for therapeutic applications. We used systematic molecular bioengineering of…”
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Intact rough‐ and smooth‐form lipopolysaccharides from Escherichia coli separated by preparative gel electrophoresis exhibit differential biologic activity in human macrophages by Pupo, Elder, Lindner, Buko, Brade, Helmut, Schromm, Andra B.

“…We established a new preparative separation procedure, based on DOC/PAGE, to isolate intact lipopolysaccharide (LPS) fractions from natural LPS preparations of…”
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Lipopolysaccharide engineering in Neisseria meningitidis: structural analysis of different pentaacyl lipid A mutants and comparison of their modified agonist properties by Pupo, Elder, Hamstra, Hendrik-Jan, Meiring, Hugo, van der Ley, Peter

“…Engineering the lipopolysaccharide (LPS) biosynthetic pathway offers the potential to obtain modified derivatives with optimized adjuvant properties. Neisseria…”
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Th17-Mediated Cross Protection against Pneumococcal Carriage by Vaccination with a Variable Antigen by Kuipers, Kirsten, Jong, Wouter S P, van der Gaast-de Jongh, Christa E, Houben, Diane, van Opzeeland, Fred, Simonetti, Elles, van Selm, Saskia, de Groot, Ronald, Koenders, Marije I, Azarian, Taj, Pupo, Elder, van der Ley, Peter, Langereis, Jeroen D, Zomer, Aldert, Luirink, Joen, de Jonge, Marien I

“…Serotype-specific protection against is an important limitation of the current polysaccharide-based vaccines. To prevent serotype replacement, reduce…”
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Isolation of smooth-type lipopolysaccharides to electrophoretic homogeneity by Pupo, Elder, Hardy, Eugenio

“…The high structural heterogeneity of smooth‐type lipopolysaccharides (LPS) enormously complicates the isolation of their constituent molecular species. Proof…”
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Isolation of smooth-type lipopolysaccharides to electrophoretic homogeneity by Pupo, Elder

“…Polyacrylamide slab gel electrophoresis in the presence of sodium dodecyl sulfate or sodium deoxycholate (SDS- or DOC-slab-PAGE) is a powerful technique for…”
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Intact rough‐ and smooth‐form lipopolysaccharides from E scherichia coli separated by preparative gel electrophoresis exhibit differential biologic activity in human macrophages by Pupo, Elder, Lindner, Buko, Brade, Helmut, Schromm, Andra B.

“…We established a new preparative separation procedure, based on DOC/ PAGE , to isolate intact lipopolysaccharide ( LPS ) fractions from natural LPS…”
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Lipopolysaccharide Engineering in Neisseria meningitidis by Pupo, Elder, Hamstra, Hendrik-Jan, Meiring, Hugo, van der Ley, Peter

“…Engineering the lipopolysaccharide (LPS) biosynthetic pathway offers the potential to obtain modified derivatives with optimized adjuvant properties. Neisseria…”
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Preparation of plasmid DNA-containing liposomes using a high-pressure homogenization–extrusion technique by Pupo, Elder, Padrón, Amalis, Santana, Enrique, Sotolongo, Jorge, Quintana, Diógenes, Dueñas, Santiago, Duarte, Carlos, de la Rosa, Maria C., Hardy, Eugenio

“…High-pressure homogenization–extrusion (HPHE) is a method that can be used for downsizing large lipid vesicles with commercially available instrumentation…”
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Linking mass spectrometry and slab-polyacrylamide gel electrophoresis by passive elution of lipopolysaccharides from reverse-stained gels: Analysis of gel-purified by Gulin, Sofia, Elder Pupo, Schweda, Elke K H, Hardy, Eugenio

“…Gulin et al apply slab-PAGE-based micropurification method to obtain unmodified lipopolysaccharides fractions of high purity from a crude LPS preparation of H…”
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Linking Mass Spectrometry and Slab-Polyacrylamide Gel Electrophoresis by Passive Elution of Lipopolysaccharides from Reverse-Stained Gels:  Analysis of Gel-Purified Lipopolysaccharides from Haemophilus influenzae Strain Rd by GULIN, Sofia, PUPO, Elder, SCHWEDA, Elke K. H., HARDY, Eugenio

“…Haemophilus influenzae is an important cause of human disease, and its lipopolysaccharide (LPS) is known to be a major virulence factor. H. influenzae produces…”
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Matrix-assisted laser desorption/ionization-time of flight-mass spectrometry of lipopolysaccharide species separated by slab-polyacrylamide gel electrophoresis: High-resolution separation and molecular weight determination of lipooligosaccharides from Vibrio fischeri strain HMK by Pupo, Elder, Phillips, Nancy J., Gibson, Bradford W., Apicella, Michael A., Hardy, Eugenio

“…We recently demonstrated that the combined use of lipopolysaccharide (LPS) reverse staining and high‐efficiency passive elution techniques can be successfully…”
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Linking Mass Spectrometry and Slab-Polyacrylamide Gel Electrophoresis by Passive Elution of Lipopolysaccharides from Reverse-Stained Gels:  Analysis of Gel-Purified Lipopolysaccharides from Haemophilus i nfluenzae Strain Rd by Gulin, Sofia, Pupo, Elder, Schweda, Elke K. H, Hardy, Eugenio

“…Haemophilus influenzae is an important cause of human disease, and its lipopolysaccharide (LPS) is known to be a major virulence factor. H. influenzae produces…”
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