Estimating transfection efficiency in differentiated and undifferentiated neural cells

Delivery of constructs for silencing or over-expressing genes or their modified versions is a crucial step for studying neuronal cell biology. Therefore, efficient transfection is important for the success of these experimental techniques especially in post-mitotic cells like neurons. In this study,...

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Bibliographic Details
Published in:	BMC research notes Vol. 12; no. 1; p. 225
Main Authors:	Alabdullah, Abeer A, Al-Abdulaziz, Basma, Alsalem, Hanan, Magrashi, Amna, Pulicat, Subramanian M, Almzroua, Amer A, Almohanna, Falah, Assiri, Abdullah Mohamed, Al Tassan, Nada A, Al-Mubarak, Bashayer R
Format:	Journal Article
Language:	English
Published:	England BioMed Central Ltd 15-04-2019 BioMed Central BMC
Subjects:	Astrocytes Binding sites Calcium phosphates Cell lines Cortex Cytological research Deoxyribonucleic acid DNA Efficiency Gene silencing Hippocampus Lipofection Methods Neuroblastoma Neuroblastoma cell lines Neurons Physiological aspects Primary cortical astrocytes Primary cortical neurons Research Note Transfection Transfection efficiency Vectors (Biology) Saudi Arabia Primary cortical neurons Lipofection Primary cortical astrocytes Neuroblastoma cell lines Transfection efficiency
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Summary:	Delivery of constructs for silencing or over-expressing genes or their modified versions is a crucial step for studying neuronal cell biology. Therefore, efficient transfection is important for the success of these experimental techniques especially in post-mitotic cells like neurons. In this study, we have assessed the transfection rate, using a previously established protocol, in both primary cortical cultures and neuroblastoma cell lines. Transfection efficiencies in these preparations have not been systematically determined before. Transfection efficiencies obtained herein were (10-12%) for neuroblastoma, (5-12%) for primary astrocytes and (1.3-6%) for primary neurons. We also report on cell-type specific transfection efficiency of neurons and astrocytes within primary cortical cultures when applying cell-type selective transfection protocols. Previous estimations described in primary cortical or hippocampal cultures were either based on general observations or on data derived from unspecified number of biological and/or technical replicates. Also to the best of our knowledge, transfection efficiency of pure primary neuronal cultures or astrocytes cultured in the context of pure or mixed (neurons/astrocytes) population cultures have not been previously determined. The transfection strategy used herein represents a convenient, and a straightforward tool for targeted cell transfection that can be utilized in a variety of in vitro applications.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1756-0500 1756-0500
DOI:	10.1186/s13104-019-4249-5