Search Results - "Puchner, Elias M."

EGF and NRG induce phosphorylation of HER3/ERBB3 by EGFR using distinct oligomeric mechanisms by van Lengerich, Bettina, Agnew, Christopher, Puchner, Elias M., Huang, Bo, Jura, Natalia

“…Heteromeric interactions between the catalytically impaired human epidermal growth factor receptor (HER3/ERBB3) and its catalytically active homologs EGFR and…”
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Single-molecule localization microscopy and tracking with red-shifted states of conventional BODIPY conjugates in living cells by Adhikari, Santosh, Moscatelli, Joe, Smith, Elizabeth M., Banerjee, Chiranjib, Puchner, Elias M.

“…Single-molecule localization microscopy (SMLM) is a rapidly evolving technique to resolve subcellular structures and single-molecule dynamics at the nanoscale…”
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Counting molecules in single organelles with superresolution microscopy allows tracking of the endosome maturation trajectory by Puchner, Elias M., Walter, Jessica M., Kasper, Robert, Bo Huang, Lim, Wendell A.

“…Cells tightly regulate trafficking of intracellular organelles, but a deeper understanding of this process is technically limited by our inability to track the…”
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Mechanoenzymatics of titin kinase by Puchner, Elias M, Alexandrovich, Alexander, Kho, Ay Lin, Hensen, Ulf, Schäfer, Lars V, Brandmeier, Birgit, Gräter, Frauke, Grubmüller, Helmut, Gaub, Hermann E, Gautel, Mathias

“…Biological responses to mechanical stress require strain-sensing molecules, whose mechanically induced conformational changes are relayed to signaling cascades…”
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Organelle interactions compartmentalize hepatic fatty acid trafficking and metabolism by Najt, Charles P., Adhikari, Santosh, Heden, Timothy D., Cui, Wenqi, Gansemer, Erica R., Rauckhorst, Adam J., Markowski, Todd W., Higgins, LeeAnn, Kerr, Evan W., Boyum, Matthew D., Alvarez, Jonas, Brunko, Sophia, Mehra, Dushyant, Puchner, Elias M., Taylor, Eric B., Mashek, Douglas G.

“…Organelle interactions play a significant role in compartmentalizing metabolism and signaling. Lipid droplets (LDs) interact with numerous organelles,…”
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Correlative Conventional and Super-resolution Photoactivated Localization Microscopy (PALM) Imaging to Characterize Chromatin Structure and Dynamics in Live Mammalian Cells by Mehra, Dushyant, Pucher, Elias M

“…A fundamental understanding of gene regulation requires a quantitative characterization of the spatial organization and dynamics of chromatin. The advent of…”
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Exploring the Conformation-Regulated Function of Titin Kinase by Mechanical Pump and Probe Experiments with Single Molecules by Puchner, Elias M, Gaub, Hermann E

“…Feel the force: A new AFM‐based single‐molecule pump‐and‐probe protocol has been used to investigate the function of different force‐induced conformations of…”
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Precisely calibrated and spatially informed illumination for conventional fluorescence and improved PALM imaging applications by Mancebo, Angel, DeMars, Luke, Ertsgaard, Christopher T, Puchner, Elias M

“…Spatial light modulation using cost efficient digital micromirror devices (DMD) is finding broad applications in fluorescence microscopy due to the reduction…”
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Efficient Cross-Correlation Filtering of One- and Two-Color Single Molecule Localization Microscopy Data by Mancebo, Angel, Mehra, Dushyant, Banerjee, Chiranjib, Kim, Do-Hyung, Puchner, Elias M

“…Single molecule localization microscopy has become a prominent technique to quantitatively study biological processes below the optical diffraction limit. By…”
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Remote control of therapeutic T cells through a small molecule–gated chimeric receptor by Wu, Chia-Yung, Roybal, Kole T., Puchner, Elias M., Onuffer, James, Lim, Wendell A.

“…There is growing interest in using engineered cells as therapeutic agents. For example, synthetic chimeric antigen receptors (CARs) can redirect T cells to…”
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Super-Resolution Microscopy: From Single Molecules to Supramolecular Assemblies by Sydor, Andrew M, Czymmek, Kirk J, Puchner, Elias M, Mennella, Vito

“…Super-resolution microscopy (SRM) methods have allowed scientists to exceed the diffraction limit of light, enabling the discovery and investigation of…”
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Nanoparticle Self-Assembly on a DNA-Scaffold Written by Single-Molecule Cut-and-Paste by Puchner, Elias M, Kufer, Stefan K, Strackharn, Mathias, Stahl, Stefan W, Gaub, Hermann E

“…Self-assembly guided by molecular recognition has in the past been employed to assemble nanoparticle superstructures like hypercrystals or nanoparticle…”
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A Conditional Gating Mechanism Assures the Integrity of the Molecular Force-Sensor Titin Kinase by Stahl, Stefan W., Puchner, Elias M., Alexandrovich, Alexander, Gautel, Mathias, Gaub, Hermann E.

“…As more and more recent investigations point out, force plays an important role in cellular regulation mechanisms. Biological responses to mechanical stress…”
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Photothermal cantilever actuation for fast single-molecule force spectroscopy by Stahl, Stefan W, Puchner, Elias M, Gaub, Hermann E

“…Photothermal cantilever excitation provides a fast and easy to implement means to control the deflection of standard atomic force microscopy cantilevers…”
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Quantitative live-cell PALM reveals nanoscopic Faa4 redistributions and dynamics on lipid droplets during metabolic transitions of yeast by Adhikari, Santosh, Moscatelli, Joe, Puchner, Elias M

“…Lipid droplets (LDs) are dynamic organelles for lipid storage and homeostasis. Cells respond to metabolic changes by regulating the spatial distribution of LDs…”
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Optically monitoring the mechanical assembly of single molecules by Gaub, Hermann E, Kufer, Stefan K, Strackharn, Mathias, Stahl, Stefan W, Gumpp, Hermann, Puchner, Elias M

“…Bottom-up assembly at the level of individual molecules requires a combination of utmost spatial precision and efficient monitoring. We have previously shown…”
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Characterizing locus specific chromatin structure and dynamics with correlative conventional and super-resolution imaging in living cells by Mehra, Dushyant, Adhikari, Santosh, Banerjee, Chiranjib, Puchner, Elias M

“…The dynamic rearrangement of chromatin is critical for gene regulation, but mapping both the spatial organization of chromatin and its dynamics remains a…”
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Force and function: probing proteins with AFM-based force spectroscopy by Puchner, Elias M, Gaub, Hermann E

“…Forces play a pivotal role in life, and the response of live systems to forces requires molecules and molecular interactions with adequate properties to…”
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ULK1 seen at the single-molecule level during autophagy initiation by Banerjee, Chiranjib, Puchner, Elias M., Kim, Do-Hyung

“…Macroautophagy/autophagy research often involves overexpressing proteins to investigate their localization, function and activity. However, this approach can…”
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Characterization of unexpected blue-shifted single molecule fluorescence of JF646 and JF669 for SMLM by Ritz, Jacob M., Khakimzhan, Aset, Kim, Do-Hyung, Puchner, Elias M.

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