Search Results - "Publicover, S J"

Patch clamp studies of human sperm under physiological ionic conditions reveal three functionally and pharmacologically distinct cation channels by Mansell, S.A., Publicover, S.J., Barratt, C.L.R., Wilson, S.M.

“…Whilst fertilizing capacity depends upon a K+ conductance (G K) that allows the spermatozoon membrane potential (V m) to be held at a negative value, the…”
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Calcium signalling in human spermatozoa: a specialized ‘toolkit’ of channels, transporters and stores by Jimenez-Gonzalez, C., Michelangeli, F., Harper, C.V., Barratt, C.L.R., Publicover, S.J.

“…Ca2+ is a ubiquitous intracellular messenger which encodes information by temporal and spatial patterns of concentration. In spermatozoa, several key…”
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Bicarbonate and bovine serum albumin reversibly ‘switch’ capacitation-induced events in human spermatozoa by Bedu-Addo, K., Lefièvre, L., Moseley, F.L.C., Barratt, C.L.R., Publicover, S.J.

“…We have investigated the reversibility of biochemical and physiological changes that occur upon suspension of ejaculated human spermatozoa during in vitro…”
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Expression of functional metabotropic glutamate receptors in primary cultured rat osteoblasts. Cross-talk with N-methyl-D-aspartate receptors by Gu, Y, Publicover, S J

“…Osteoblasts and osteoclasts express functional N-methyl-d-aspartate (NMDA) receptors, which participate in regulation of bone matrix. In rat femoral…”
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Protein tyrosine phosphorylation, hyperactivation and progesterone-induced acrosome reaction are enhanced in IVF media: an effect that is not associated with an increase in protein kinase A activation by Moseley, F.L.C., Jha, K.N., Björndahl, Lars, Brewis, I.A., Publicover, S.J., Barratt, C.L.R., Lefièvre, L.

“…Sperm capacitation is a prerequisite for successful in vitro fertilization (IVF) and therefore a focus of sperm preparation in IVF laboratories. The technology…”
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Calcium-channel activation and matrix protein upregulation in bone cells in response to mechanical strain by Walker, L. M., Publicover, S. J., Preston, M. R., Said Ahmed, M. A. A., El Haj, A. J.

“…Femur‐derived osteoblasts cultured from rat femora were loaded with Fluo‐3 using the AM ester. A quantifiable stretch was applied and [Ca2+]i levels monitored…”
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Multi-state, 4-aminopyridine-sensitive ion channels in human spermatozoa by Gu, Y., Kirkman-Brown, J.C., Korchev, Y., Barratt, C.L.R, Publicover, S.J.

“…Although ion channels are known to be pivotal to sperm function, the technical difficulty of applying electrophysiological techniques to spermatozoa has…”
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Biphasic elevation of [Ca(2+)](i) in individual human spermatozoa exposed to progesterone by Kirkman-Brown, J C, Bray, C, Stewart, P M, Barratt, C L, Publicover, S J

“…Fluorimetric studies on progesterone-induced [Ca(2+)](i) signalling in mammalian spermatozoa show both the well-characterised [Ca(2+)](i) transient and a…”
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Voltage-operated Ca2+ channels and the acrosome reaction: which channels are present and what do they do? by Publicover, S.J., Barratt, C.L.R.

“…Evidence from pharmacological studies suggests that induction of the acrosome reaction of mammalian spermatozoa by solubilized zona pellucida, and possibly by…”
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Mechanotransduction pathways in bone : calcium fluxes and the role of voltage-operated calcium channels by EL HAJ, A. J, WALKER, L. M, PRESTON, M. R, PUBLICOVER, S. J

“…Changes in strain distribution across the vertebrate skeleton induce modelling and remodelling of bone structure. This relationship, like many in biomedical…”
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Three types of K + currents in murine osteocyte-like cells (MLO-Y4) by Gu, Y, Preston, M.R, El Haj, A.J, Howl, J.D, Publicover, S.J

“…Osteocytes play an important role in signaling within bone. Communication of osteocytes with each other and with bone lining cells may have a function in…”
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Chloride channels join the sperm ‘channelome’ by Publicover, S. J., Barratt, Christopher L. R.

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The NMDA type glutamate receptors expressed by primary rat osteoblasts have the same electrophysiological characteristics as neuronal receptors by Gu, Y, Genever, P G, Skerry, T M, Publicover, S J

“…Cells of mammalian bone express glutamate receptors. Functional N-methyl-D-aspartate (NMDA) receptors have been demonstrated in human, osteoblastic MG-63…”
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Sperm motility: things are moving in the lab by Publicover, S.J., Barratt, Christopher L.R.

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Patterns of [Ca2+](i) mobilization and cell response in human spermatozoa exposed to progesterone by Bedu-Addo, K, Barratt, C L R, Kirkman-Brown, J C, Publicover, S J

“…Human spermatozoa stimulated with progesterone (a product of the cumulus and thus encountered by sperm prior to fertilization in vivo) apparently mobilize…”
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Inhibitors of receptor tyrosine kinases do not suppress progesterone-induced [Ca2+]i signalling in human spermatozoa by Kirkman-Brown, J.C., Lefièvre, L., Bray, C., Stewart, P.M., Barratt, C.L.R., Publicover, S.J.

“…Previous studies have implicated receptor tyrosine kinases in progesterone-induced [Ca2+]i signalling, and consequent induction of the acrosome reaction, in…”
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Interaction between sperm and zona pellucida in male fertility by Barratt, Christopher LR, Publicover, Stephen J

“…21 years ago Jim Overstreet identified defects in the ability of sperm to bind to and penetrate the zona pellucida in three men with normal semen variables.'…”
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Patterns of [Ca2+]i mobilization and cell response in human spermatozoa exposed to progesterone by Bedu-Addo, K., Barratt, C.L.R., Kirkman-Brown, J.C., Publicover, S.J.

“…Human spermatozoa stimulated with progesterone (a product of the cumulus and thus encountered by sperm prior to fertilization in vivo) apparently mobilize Ca2+…”
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Nifedipine reveals the existence of two discrete components of the progesterone-induced [Ca 2+] i transient in human spermatozoa by Kirkman-Brown, J.C, Barratt, C.L.R, Publicover, S.J

“…The steroid progesterone, an agonist of acrosome reaction, induces a biphasic [Ca 2+] i-signal in human sperm comprising an initial transient [Ca 2+] i…”
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Voltage-dependent potentiation of low-voltage-activated Ca2+ channel currents in cultured rat bone marrow cells by Publicover, S J, Preston, M R, El Haj, A J

“…1. The whole-cell patch-clamp technique was used to study Ca2+ channel currents in stromal cells of 7-10 day dexamethasone-treated and control rat bone marrow…”
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