Search Results - "Przybycien, Todd M"

Alternative bioseparation operations: life beyond packed-bed chromatography by Przybycien, Todd M, Pujar, Narahari S, Steele, Landon M

“…Chromatography is undoubtedly the workhorse of downstream processes, affording high resolution for bioseparations. At the same time, it has the notoriety of…”
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Rapid In-Process Measurement of Live Virus Vaccine Potency Using Laser Force Cytology: Paving the Way for Rapid Vaccine Development by McCracken, Reilly, Al-Nazal, Noor, Whitmer, Travis, Yi, Sijia, Wagner, James M, Hebert, Colin G, Lowry, Matthew J, Hayes, Peter R, Schneider, James W, Przybycien, Todd M, Mukherjee, Malini

“…Vaccinations to prevent infectious diseases are given to target the body’s innate and adaptive immune systems. In most cases, the potency of a live virus…”
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Aggregation of lysozyme and of poly(ethylene glycol)-modified lysozyme after adsorption to silica by Daly, Susan M., Przybycien, Todd M., Tilton, Robert D.

“…Surface-induced aggregation is a common instability during protein storage, delivery and purification. This aggregation can lead to the formation of fibrils…”
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Adsorption of Poly(ethylene glycol)-Modified Lysozyme to Silica by Daly, Susan M, Przybycien, Todd M, Tilton, Robert D

“…Covalent grafting of poly(ethylene glycol) (PEG) to pharmaceutical proteins, “PEGylation”, is becoming more commonplace due to improved therapeutic efficacy…”
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Viral adventitious agent detection using laser force cytology: Intrinsic cell property changes with infection and comparison to in vitro testing by Hayes, Peter R., Przybycien, Todd M., Schneider, James W.

“…Adventitious agent testing in biomanufacturing requires assays of broad detection capability to screen for as many infectious agents as possible. The current…”
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High throughput solubility and redissolution screening for antibody purification via combined PEG and zinc chloride precipitation by Gu, Qin, Li, Zhao, Coffman, Jonathan L., Przybycien, Todd M., Zydney, Andrew L.

“…As upstream product titers increase, the downstream chromatographic capture step has become a significant “downstream bottleneck.” Precipitation becomes more…”
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Continuous precipitation‐filtration process for initial capture of a monoclonal antibody product using a four‐stage countercurrent hollow fiber membrane washing step by Minervini, Mirko, Mergy, Matthew, Zhu, Yuncan, Gutierrez Diaz, Mario A., Pointer, Craig, Shinkazh, Oleg, Oppenheim, Sheldon F., Cramer, Steven M., Przybycien, Todd M., Zydney, Andrew L.

“…The significant increase in product titers, coupled with the growing focus on continuous bioprocessing, has renewed interest in using precipitation as a…”
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Surfactant-induced Marangoni transport of lipids and therapeutics within the lung by Stetten, Amy Z., Iasella, Steven V., Corcoran, Timothy E., Garoff, Stephen, Przybycien, Todd M., Tilton, Robert D.

“…Understanding the fundamentals of surface transport on thin viscous films has important application in pulmonary drug delivery. The human lung contains a…”
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Flow regime transitions and effects on solute transport in surfactant-driven Marangoni flows by Iasella, Steven V., Sun, Ningguan, Zhang, Xin, Corcoran, Timothy E., Garoff, Stephen, Przybycien, Todd M., Tilton, Robert D.

“…[Display omitted] Surfactant-driven Marangoni flow on liquid films is predicted to depend on subphase depth and initial surface tension difference between the…”
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Critical factors for high-performance physically adsorbed (dynamic) polymeric wall coatings for capillary electrophoresis of DNA by Doherty, Erin A. S., Berglund, K. Derek, Buchholz, Brett A., Kourkine, Igor V., Przybycien, Todd M., Tilton, Robert D., Barron, Annelise E.

“…Physically adsorbed (dynamic) polymeric wall coatings for microchannel electrophoresis have distinct advantages over covalently linked coatings. In order to…”
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The Conformation of the Poly(ethylene glycol) Chain in Mono-PEGylated Lysozyme and Mono-PEGylated Human Growth Hormone by Pai, Sheetal S, Hammouda, Boualem, Hong, Kunlun, Pozzo, Danilo C, Przybycien, Todd M, Tilton, Robert D

“…Covalent conjugation of poly­(ethylene glycol) or “PEGylation” has proven an effective strategy to improve pharmaceutical protein efficacy by hindering…”
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Effect of polyelectrolyte–surfactant complexation on Marangoni transport at a liquid–liquid interface by Dunér, Gunnar, Kim, Michelle, Tilton, Robert D., Garoff, Stephen, Przybycien, Todd M.

“…[Display omitted] •Polymer complexation alters surfactant-induced Marangoni flow at oil/water interface.•Polymer adsorption irreversibility creates hysteresis…”
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Transport of a partially wetted particle at the liquid/vapor interface under the influence of an externally imposed surfactant generated Marangoni stress by Sharma, Ramankur, Corcoran, Timothy E., Garoff, Stephen, Przybycien, Todd M., Tilton, Robert D.

“…[Display omitted] •Spatiotemporally evolving surface tension & liquid velocity control particle dynamics.•Subphase depth, particle size and wettability control…”
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Moringa oleifera Seed Protein Adsorption to Silica: Effects of Water Hardness, Fractionation, and Fatty Acid Extraction by Nordmark, Brittany A, Bechtel, Toni M, Riley, John K, Velegol, Darrell, Velegol, Stephanie B, Przybycien, Todd M, Tilton, Robert D

“…Motivated by the proposed use of cationic protein-modified sand for water filtration in developing nations, this study concerns the adsorption of Moringa…”
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Enabling Marangoni flow at air-liquid interfaces through deposition of aerosolized lipid dispersions by Stetten, Amy Z., Moraca, Grace, Corcoran, Timothy E., Tristram-Nagle, Stephanie, Garoff, Stephen, Przybycien, Todd M., Tilton, Robert D.

“…[Display omitted] It has long been known that deposited drops of surfactant solution induce Marangoni flows at air-liquid interfaces. These surfactant drops…”
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Separation of PEGylated variants of ribonuclease A and apo-α-lactalbumin via reversed phase chromatography by Cisneros-Ruiz, Mayra, Mayolo-Deloisa, Karla, Rito-Palomares, Marco, Przybycien, Todd M.

“…•The potential of RPC for separation of PEGylated proteins was exploited.•PEGylated variants of RNase A and apo-α-lactalbumin were separated.•The monoPEGylated…”
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Transient Marangoni transport of colloidal particles at the liquid/liquid interface caused by surfactant convective-diffusion under radial flow by Dunér, Gunnar, Garoff, Stephen, Przybycien, Todd M., Tilton, Robert D.

“…[Display omitted] Interfacial tension gradients at a liquid/liquid interface drive Marangoni flows. When colloidal particles are adsorbed to an interface in…”
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Effect of humic acids on the kaolin coagulation performance of Moringa oleifera proteins by Nordmark, Brittany A., Przybycien, Todd M., Tilton, Robert D.

“…Cationic proteins from Moringa oleifera seeds are of interest as sustainable coagulants for drinking water treatment in regions with poor access to potable…”
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Evolution and disappearance of solvent drops on miscible polymer subphases by Stetten, Amy Z., Treece, Bradley W., Corcoran, Timothy E., Garoff, Stephen, Przybycien, Todd M., Tilton, Robert D.

“…[Display omitted] Traditionally, an interface is defined as a boundary between immiscible phases. However, previous work has shown that even when two fluids…”
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Aerosolizing Lipid Dispersions Enables Antibiotic Transport Across Mimics of the Lung Airway Surface Even in the Presence of Pre-existing Lipid Monolayers by Iasella, Steven V, Stetten, Amy Z, Corcoran, Timothy E, Garoff, Stephen, Przybycien, Todd M, Tilton, Robert D

“…Secondary lung infections are the primary cause of morbidity associated with cystic fibrosis lung disease. Aerosolized antibiotic inhalation is potentially…”
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