Search Results - "Pruitt, Steven"

DNA replication stress restricts ribosomal DNA copy number by Salim, Devika, Bradford, William D, Freeland, Amy, Cady, Gillian, Wang, Jianmin, Pruitt, Steven C, Gerton, Jennifer L

“…Ribosomal RNAs (rRNAs) in budding yeast are encoded by ~100-200 repeats of a 9.1kb sequence arranged in tandem on chromosome XII, the ribosomal DNA (rDNA)…”
Get full text

Dual Roles of Poly(dA:dT) Tracts in Replication Initiation and Fork Collapse by Tubbs, Anthony, Sridharan, Sriram, van Wietmarschen, Niek, Maman, Yaakov, Callen, Elsa, Stanlie, Andre, Wu, Wei, Wu, Xia, Day, Amanda, Wong, Nancy, Yin, Mianmian, Canela, Andres, Fu, Haiqing, Redon, Christophe, Pruitt, Steven C., Jaszczyszyn, Yan, Aladjem, Mirit I., Aplan, Peter D., Hyrien, Olivier, Nussenzweig, André

“…Replication origins, fragile sites, and rDNA have been implicated as sources of chromosomal instability. However, the defining genomic features of replication…”
Get full text

A Signature of Genomic Instability Resulting from Deficient Replication Licensing by Pruitt, Steven C, Qin, Maochun, Wang, Jianmin, Kunnev, Dimiter, Freeland, Amy

“…Insufficient licensing of DNA replication origins has been shown to result in genome instability, stem cell deficiency, and cancers. However, it is unclear…”
Get full text

Reduced Mcm2 Expression Results in Severe Stem/Progenitor Cell Deficiency and Cancer by Pruitt, Steven C., Bailey, Kimberly J., Freeland, Amy

“…Mcm2 is a component of the DNA replication licensing complex that marks DNA replication origins during G1 of the cell cycle for use in the subsequent S‐phase…”
Get full text

Neural Stem Cell Detection, Characterization, and Age-Related Changes in the Subventricular Zone of Mice by Maslov, Alexander Y, Barone, Tara A, Plunkett, Robert J, Pruitt, Steven C

“…The mammalian brain contains neural stem cells (NSCs) that allow continued neurogenesis throughout the life of the animal. However, neurogenesis is known to…”
Get full text

Whole-genome sequencing identifies genomic heterogeneity at a nucleotide and chromosomal level in bladder cancer by Morrison, Carl D, Liu, Pengyuan, Woloszynska-Read, Anna, Zhang, Jianmin, Luo, Wei, Qin, Maochun, Bshara, Wiam, Conroy, Jeffrey M, Sabatini, Linda, Vedell, Peter, Xiong, Donghai, Liu, Song, Wang, Jianmin, Shen, He, Li, Yinwei, Omilian, Angela R, Hill, Annette, Head, Karen, Guru, Khurshid, Kunnev, Dimiter, Leach, Robert, Eng, Kevin H, Darlak, Christopher, Hoeflich, Christopher, Veeranki, Srividya, Glenn, Sean, You, Ming, Pruitt, Steven C, Johnson, Candace S, Trump, Donald L

“…Using complete genome analysis, we sequenced five bladder tumors accrued from patients with muscle-invasive transitional cell carcinoma of the urinary bladder…”
Get full text

Post-transcriptional homeostasis and regulation of MCM2-7 in mammalian cells by Chuang, Chen-Hua, Yang, Dian, Bai, Gongshi, Freeland, Amy, Pruitt, Steven C, Schimenti, John C

“…The MiniChromosome Maintenance 2-7 (MCM2-7) complex provides essential replicative helicase function. Insufficient MCMs impair the cell cycle and cause genomic…”
Get full text

Cdkn1b overexpression in adult mice alters the balance between genome and tissue ageing by Pruitt, Steven C., Freeland, Amy, Rusiniak, Michael E., Kunnev, Dimiter, Cady, Gillian K.

“…Insufficient cell proliferation has been suggested as a potential cause of age-related tissue dysgenesis in mammals. However, genetic manipulation of cell…”
Get full text

Effect of minichromosome maintenance protein 2 deficiency on the locations of DNA replication origins by Kunnev, Dimiter, Freeland, Amy, Qin, Maochun, Leach, Robert W, Wang, Jianmin, Shenoy, Rajani M, Pruitt, Steven C

“…Minichromosome maintenance (MCM) proteins are loaded onto chromatin during G1-phase and define potential locations of DNA replication initiation. MCM protein…”
Get full text

Cell Cycle Heterogeneity in the Small Intestinal Crypt and Maintenance of Genome Integrity by Pruitt, Steven C., Freeland, Amy, Kudla, Angela

“…Stem cell quiescence has been hypothesized to suppress the rate at which genetic mutations accumulate within tissues by reducing the number of divisions a cell…”
Get full text

A unique mutator phenotype reveals complementary oncogenic lesions leading to acute leukemia by Yin, Mianmian, Baslan, Timour, Walker, Robert L, Zhu, Yuelin J, Freeland, Amy, Matsukawa, Toshihiro, Sridharan, Sriram, Nussenzweig, André, Pruitt, Steven C, Lowe, Scott W, Meltzer, Paul S, Aplan, Peter D

“…Mice homozygous for a hypomorphic allele of DNA replication factor minichromosome maintenance protein 2 (designated Mcm2cre/cre) develop precursor T cell…”
Get full text

Yeast two-hybrid interaction partner screening through in vivo Cre-mediated Binary Interaction Tag generation by Hastie, Alex R., Pruitt, Steven C.

“…Yeast two-hybrid (Y2H) has been successfully used for genome-wide screens to identify protein–protein interactions for several model organisms. Nonetheless,…”
Get full text

Accumulation of mutations and somatic selection in aging neural stem/progenitor cells by Bailey, Kimberly J., Maslov, Alexander Y., Pruitt, Steven C.

“…Summary Genomic instability within somatic stem cells may lead to the accumulation of mutations and contribute to cancer or other age‐related phenotypes…”
Get full text

Stem/Progenitor Cell‐Specific Enhanced Green Fluorescent Protein Expression Driven by the Endogenous Mcm2 Promoter by Maslov, Alexander Y., Bailey, Kimberly J., Mielnicki, Lawrence M., Freeland, Amy L., Sun, Xiaolei, Burhans, William C., Pruitt, Steven C.

“…Previous studies have demonstrated expression of the minichromosome maintenance protein Mcm2 in cells that remain competent to divide, including…”
Get full text

Determination of transcription factors and their possible roles in the regulation of Pax3 gene expression in the mouse B16 F1 melanoma cell line by Zhu, Bi-ke, Pruitt, Steven C

“…The objective of this study was to determine which transcription factors regulate the expression of the Pax3 gene in the mouse B16 F1 melanoma cell line. The…”
Get full text

Analysis of fluorescent protein expressing cells by flow cytometry by Pruitt, Steven C, Mielnicki, Lawrence M, Stewart, Carleton C

“…The process for transfection of cells with expression and gene-trap vectors expressing fluorescent reporter proteins is described. The measurement and sorting…”
Get more information

Privileged scaffolds for blocking protein–protein interactions: 1,4-disubstituted naphthalene antagonists of transcription factor complex HOX–PBX/DNA by Ji, Tao, Lee, Madison, Pruitt, Steven C., Hangauer, David G.

“…Structure-based-design studies, based upon the crystal structure of the HOXB1–PBX1/DNA transcription factor complex, were used to identify 1,4-disubstituted…”
Get full text

Hox/Pbx and Brn binding sites mediate Pax3 expression in vitro and in vivo by Pruitt, Steven C., Bussman, Amy, Maslov, Alexander Y., Natoli, Thomas A., Heinaman, Roy

“…Pax3 is a paired-homeodomain class transcription factor that serves a role in dorsal–ventral and medial–lateral patterning during vertebrate embryogenesis. Its…”
Get full text

Repurposing a Cyclin-Dependent Kinase 1 (CDK1) Mitotic Regulatory Network to Complete Terminal Differentiation in Lens Fiber Cells by Taylor, Allen, Gu, Yumei, Chang, Min-Lee, Yang, Wenxin, Francisco, Sarah, Rowan, Sheldon, Bejarano, Eloy, Pruitt, Steven, Zhu, Liang, Weiss, Grant, Brennan, Lisa, Kantorow, Marc, Whitcomb, Elizabeth A

“…During lens fiber cell differentiation, organelles are removed in an ordered manner to ensure lens clarity. A critical step in this process is removal of the…”
Get full text

Mice with a Unique Mutator Phenotype Allow Detection of Lymphoid Leukemia Tumor Suppressor Genes by Yin, Mianmian, Baslan, Timour, Freeland, Amy, Matsukawa, Toshihiro, Pruitt, Steven C., Aplan, Peter D.

“…Mice that are homozygous for a hypomorphic allele of the DNA replication factor minichromosome maintenance protein 2 (designated Mcm2hypo) on a C57Bl/6…”
Get full text