Search Results - "Protein expression and purification"

Optimizing high-yield production of SARS-CoV-2 soluble spike trimers for serology assays by Esposito, Dominic, Mehalko, Jennifer, Drew, Matthew, Snead, Kelly, Wall, Vanessa, Taylor, Troy, Frank, Peter, Denson, John-Paul, Hong, Min, Gulten, Gulcin, Sadtler, Kaitlyn, Messing, Simon, Gillette, William

“…The SARS-CoV-2 spike trimer is the primary antigen for several serology assays critical to determining the extent of SARS-CoV-2 exposure in the population…”
Get full text

Recombinant expression of nanobodies and nanobody-derived immunoreagents by de Marco, Ario

“…Antibody fragments for which the sequence is available are suitable for straightforward engineering and expression in both eukaryotic and prokaryotic systems…”
Get full text

A brief practical review of size exclusion chromatography: Rules of thumb, limitations, and troubleshooting by Burgess, Richard R.

“…Through years of research, teaching, troubleshooting, and editing, I have developed a solid working understanding of many aspects of protein purification and…”
Get full text

An overview of enzymatic reagents for the removal of affinity tags by Waugh, David S.

“…► I review enzymes commonly used to remove affinity tags. ► Both endoproteases and exoproteases are covered. ► The specificity and biochemical properties of…”
Get full text

Optimization of protein purification and characterization using Thermofluor screens by Boivin, Stephane, Kozak, Sandra, Meijers, Rob

“…•Optimization procedures for sample purification and characterization.•Rational design of buffers and additive screens compatible with Thermofluor…”
Get full text

Recombinant production of antimicrobial peptides in Escherichia coli: A review by Li, Yifeng

“…► This article reviews commonly used strategies for antimicrobial peptides production in E. coli. ► Possible optimizations at different stages are briefly…”
Get full text

Expression and purification of recombinant G protein-coupled receptors: A review by Wiseman, Daniel N., Otchere, Abigail, Patel, Jaimin H., Uddin, Romez, Pollock, Naomi L., Routledge, Sarah J., Rothnie, Alice J., Slack, Cathy, Poyner, David R., Bill, Roslyn M., Goddard, Alan D.

“…Given their extensive role in cell signalling, GPCRs are significant drug targets; despite this, many of these receptors have limited or no available…”
Get full text

Yeast-expressed recombinant SARS-CoV-2 receptor binding domain RBD203-N1 as a COVID-19 protein vaccine candidate by Chen, Wen-Hsiang, Pollet, Jeroen, Strych, Ulrich, Lee, Jungsoon, Liu, Zhuyun, Kundu, Rakhi Tyagi, Versteeg, Leroy, Villar, Maria Jose, Adhikari, Rakesh, Wei, Junfei, Poveda, Cristina, Keegan, Brian, Bailey, Aaron Oakley, Chen, Yi-Lin, Gillespie, Portia M., Kimata, Jason T., Zhan, Bin, Hotez, Peter J., Bottazzi, Maria Elena

“…SARS-CoV-2 protein subunit vaccines are currently being evaluated by multiple manufacturers to address the global vaccine equity gap, and need for low-cost,…”
Get full text

Linkers: A synergistic way for the synthesis of chimeric proteins by Patel, Dharti Keyur, Menon, Dhanya V., Patel, Darshan H., Dave, Gayatri

“…In the cell, the protein domains are attached with the short oligopeptide, commonly known as linker peptide. Besides bridging, the linker assists in the…”
Get full text

Expression, purification and characterization of a recombinant antimicrobial peptide Hispidalin in Pichia pastoris by Meng, De-Mei, Li, Wen-Juan, Shi, Lin-Yue, Lv, Yu-Jie, Sun, Xue-Qing, Hu, Jin-Cheng, Fan, Zhen-Chuan

“…Hispidalin is a novel antimicrobial peptide isolated from the seeds of Benincasa hispida and is reported to have broad antimicrobial activity against various…”
Get full text

Expression of SARS-CoV-2 surface glycoprotein fragment 319–640 in E. coli, and its refolding and purification by Fitzgerald, Gabriel A., Komarov, Andrei, Kaznadzey, Anna, Mazo, Ilya, Kireeva, Maria L.

“…Sensitive and specific serology tests are essential for epidemiological and public health studies of COVID-19 and for vaccine efficacy testing. The presence of…”
Get full text

Biotechnological applications of elastin-like polypeptides and the inverse transition cycle in the pharmaceutical industry by Fletcher, Emmanuella E, Yan, Dandan, Kosiba, Anthony A, Zhou, Yang, Shi, Haifeng

“…Proteins are essential throughout the biological and biomedical sciences and the purification strategies of proteins of interest have advanced over centuries…”
Get full text

Escherichia coli as a versatile cell factory: Advances and challenges in recombinant protein production by İncir, İbrahim, Kaplan, Özlem

“…E. coli plays a substantial role in recombinant protein production. Its importance increased with the discovery of recombinant DNA technology and the…”
Get full text

Development of the Twin-Strep-tag® and its application for purification of recombinant proteins from cell culture supernatants by Schmidt, Thomas G.M., Batz, Lilia, Bonet, Lidia, Carl, Uwe, Holzapfel, Gerd, Kiem, Klaus, Matulewicz, Kamila, Niermeier, Dennis, Schuchardt, Isabel, Stanar, Kristian

“…•Twin-Strep-tag® (TST) binds to a single Strep-Tactin® tetramer.•TST arrangement provides enhanced affinity by off-rate reduction.•TST binds reversibly and…”
Get full text

Improved production of SARS-CoV-2 spike receptor-binding domain (RBD) for serology assays by Mehalko, Jennifer, Drew, Matthew, Snead, Kelly, Denson, John-Paul, Wall, Vanessa, Taylor, Troy, Sadtler, Kaitlyn, Messing, Simon, Gillette, William, Esposito, Dominic

“…The receptor-binding domain (RBD) of the SARS-CoV-2 spike protein is a commonly used antigen for serology assays critical to determining the extent of…”
Get full text

Overcoming challenges for amplified expression of recombinant proteins using Escherichia coli by Ahmad, Irshad, Nawaz, Nighat, Darwesh, Nizam M., ur Rahman, Sadeeq, Mustafa, Mohammad Z., Khan, Sher B., Patching, Simon G.

“…A thorough characterisation of the genetics, physiology and metabolism of Escherichia coli has led to the availability of a large number of strains and vectors…”
Get full text

Improved self-cleaving precipitation tags for efficient column free bioseparations by Yuan, Hongyu, Prabhala, Sai Vivek, Coolbaugh, Michael J., Stimple, Samuel D., Wood, David W.

“…Current biological research requires simple protein bioseparation methods capable of purifying target proteins in a single step with high yields and purities…”
Get full text

A new insight into purification of polyphenol oxidase and inhibition effect of curcumin and quercetin on potato polyphenol oxidase by Aksoy, Mine

“…In the literature, the polyphenol oxidase (PPO) enzyme has been purified a many times via Sepharose 4B-l-tyrosine-p-aminobenzoic acid affinity column. In order…”
Get full text

Nature of recombinant human serum amyloid A1 in Escherichia coli and its preferable approach for purification by Ahmad, Saira, Gardner, Qurratulann Afza, Shakir, Nisar Ahmad, Gulzar, Sabahat, Azim, Naseema, Akhtar, Muhammad

“…Serum amyloid A1 (SAA1) is an apolipoprotein which is involved in amyloid A amyloidosis (AA) by forming fibrils. The process of fibrillation is still being…”
Get full text

Thermostable phenylacetic acid degradation protein TtPaaI from Thermus thermophilus as a scaffold for tetravalent display of proteins by Chorążewska, Aleksandra, Regan, Darragh, Kalka, Marta, Ciura, Krzysztof, Porębska, Natalia, Łukasz Opaliński

“…Numerous proteins in nature strictly require oligomerization for their full activity. Moreover, the function of natural and artificial proteins can me adjusted…”
Get full text