Genome sequence of Acremonium strictum AAJ6 strain isolated from the Cerrado biome in Brazil and CAZymes expression in thermotolerant industrial yeast for ethanol production

Genome sequence of Acremonium strictum AAJ6 strain isolated from the Cerrado biome in Brazil and CAZymes expression in thermotolerant industrial yeast for ethanol production

[Display omitted] •Acreminum strictum AAJ6 isolated from the Cerrado biome in Brazil.•AAJ6 genome showed 380 carbohydrate-active enzyme domains predicted to be secreted.•Expression of GH74 and GH3 in thermotolerant Saccharomyces cerevisiae strains.•Genome-integration in thermotolerant industrial yea...

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Published in:Process biochemistry (1991) Vol. 98; pp. 139 - 150
Main Authors: Lopes, Alberto Moura Mendes, Félix de Mélo, Allan Henrique, Procópio, Dielle Pierroti, Teixeira, Gleidson Silva, Carazzolle, Marcelo F., de Carvalho, Lucas Miguel, Adelantado, Núria, Pereira, Gonçalo A.G., Ferrer, Pau, Filho, Francisco Maugeri, Goldbeck, Rosana
Format: Journal Article
Language:English
Published: Barking Elsevier Ltd 01-11-2020
Elsevier BV
Subjects:
Acremonium strictum
Acremonium strictum genome sequence
Bagasse
Bioethanol
Biofuels
Cassettes
Cellobiase
Cellobiose
Cellulolytic and ligninolytic enzymes
Degradation
Enzymes
Ethanol
Fungi
Gene sequencing
Genomes
Glucosidase
Glycosidases
Glycoside hydrolase
Hydrolase
Industrial strains
Lignocellulose
Nucleotide sequence
Plant debris
Substrates
Sugarcane
Superhigh frequencies
Temperature tolerance
Thermotolerant yeast
Tropical savanna
Whole genome sequencing
Yeasts
β-Glucosidase
Brazil
Tropical savanna
Cellulolytic and ligninolytic enzymes
Bioethanol
Thermotolerant yeast
Acremonium strictum genome sequence
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Summary:[Display omitted] •Acreminum strictum AAJ6 isolated from the Cerrado biome in Brazil.•AAJ6 genome showed 380 carbohydrate-active enzyme domains predicted to be secreted.•Expression of GH74 and GH3 in thermotolerant Saccharomyces cerevisiae strains.•Genome-integration in thermotolerant industrial yeast results in higher ethanol.•Reduction in cellulose hydrolysis time gives increased ethanol production. Increased demand for biofuels promotes the search for new biomass-degrading fungi. Acremonium strictum is an environmentally widespread filamentous fungi found on plant debris; that secretes lignocellulose-degrading enzymes. A recently isolated A. strictum strain, AAJ6; native to the Brazilian Cerrado biome was evaluated for its capacity to degrade lignocellulosic substrates. In this study, whole-genome sequencing of AAJ6 was performed and 775 CAZy domains were identified which correlated to those of A. strictum strain DS1bioAY4a and other lignocellulolytic fungi; suggesting AAJ6 is a high CAZyme producer. We expressed the glycoside hydrolase families GH74 and GH3 from plasmid or genome-integrated to evaluate the ethanol production from cellulosic substrates in Brazilian industrial Saccharomyces cerevisiae strains (PE-2 and SA-1) evolved for thermotolerance (AMY12 and AMY35). Those expressing the genome-integrated enzymes showed the highest β-glucosidase activity and growth in medium with cellobiose at 40°C. The strain AGY005 (integrated cassettes) showed 19, 23 and 46% higher ethanol production in SHF, pSSF (partial hydrolysis SSF) and SSF processes, respectively, using Avicel, and ∼50% more ethanol using pre-treated sugarcane bagasse, compared to the strain with a plasmid-based expression. These results indicate the improved performance of thermotolerant industrial strains with genome-integrated CAZymes in the SSF process for 2G ethanol.
ISSN:1359-5113
1873-3298
DOI:10.1016/j.procbio.2020.07.029