Detection of multiple toxic agents using a planar array immunosensor

Detection of multiple toxic agents using a planar array immunosensor

A planar array immunosensor, equipped with a charge-coupled device (CCD) as a detector, was used to simultaneously detect 3 toxic analytes. Wells approximately 2 mm in diameter were formed on glass slides using a photoactivated optical adhesive. Antibodies against staphylococcal enterotoxin B (SEB),...

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Bibliographic Details
Published in:Biosensors & bioelectronics Vol. 13; no. 3; pp. 407 - 415
Main Authors: Wadkins, Randy M., Golden, Joel P., Pritsiolas, Leo M., Ligler, Frances S.
Format: Journal Article
Language:English
Published: Lausanne Elsevier B.V 01-03-1998
Elsevier Science
Subjects:
array
Biological and medical sciences
Biosensing Techniques
biosensor
Biosensors
Biotechnology
CCD
Disposable Equipment
Double-Blind Method
fluorescence
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Image Processing, Computer-Assisted
Immunoassay
Methods. Procedures. Technologies
multianalyte
Optics and Photonics
Various methods and equipments
biosensor
multianalyte
CCD
fluorescence
array
immunoassay
Performance evaluation
Disposable
Image analysis
Waveguide array
Biosensor
Charge coupled device
Fluorescence
Antigen antibody reaction
Poison
Detection
Immunosensor
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Summary:A planar array immunosensor, equipped with a charge-coupled device (CCD) as a detector, was used to simultaneously detect 3 toxic analytes. Wells approximately 2 mm in diameter were formed on glass slides using a photoactivated optical adhesive. Antibodies against staphylococcal enterotoxin B (SEB), ricin, and Yersinia pestis were covalently attached to the bottoms of the circular wells to form the sensing surface. Rectangular wells containing chicken immunoglobulin were used as alignment markers and to generate control signals. After removing the optical adhesive, the slides were mounted over a scientific grade CCD operating at ambient temperature in inverted (multipin phasing) mode. A two-dimensional graded index of refraction lens array was used to focus the sensing surface onto the CCD. Solutions of toxins were then placed on the slide. After rinsing, Cy5-labeled antibodies were introduced. The identity and amount of toxin bound at each location on the slide were determined by quantitative image analysis. Concentrations as low as 25 ng/mL of ricin, 15 ng/mL of pestis F1 antigen, and 5 ng/mL of SEB could be routinely measured.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
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ISSN:0956-5663
1873-4235
DOI:10.1016/S0956-5663(97)00113-9