Search Results - "Prestegard, J.H"

Structural Monitoring of Oligosaccharides through 13C Enrichment and NMR Observation of Acetyl Groups by Yu, Fei, Prestegard, J.H.

“…Structural characterization of biomolecules by NMR methods frequently requires the enrichment of magnetically active isotopes at particular molecular sites…”
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Residual dipolar coupling derived orientational constraints on ligand geometry in a 53 kDa protein-ligand complex by Bolon, P.J, Al-Hashimi, H.M, Prestegard, J.H

“…The geometric relationships between ligands and the functional groups that bind ligands in soluble ligand-protein complexes have traditionally been deduced…”
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Quantitation of rapid proton-deuteron amide exchange using hadamard spectroscopy by Bougault, Catherine, Feng, Lianmei, Glushka, John, Kupce, Eriks, Prestegard, J H

“…The rates of amide proton exchange in protein backbones are very useful reporters of accessibility and structural stability of specific residues and secondary…”
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Direct NOE simulation from long MD trajectories by Chalmers, G., Glushka, J.N., Foley, B.L., Woods, R.J., Prestegard, J.H.

“…[Display omitted] •A software package is presented which allows calculation of NOEs from long MD trajectories.•The package avoids commonly used assumptions…”
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New techniques in structural NMR — anisotropic interactions by Prestegard, J.H.

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Exchange facilitated indirect detection of hyperpolarized 15ND 2-amido-glutamine by Barb, A.W., Hekmatyar, S.K., Glushka, J.N., Prestegard, J.H.

“…[Display omitted] . ► Deuteration of [15]N-glutamine reduces R 1 ∼3-fold. ► Solvent catalyzed D/H exchange permits indirect detection of [15]N polarization. ►…”
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Probing alanine transaminase catalysis with hyperpolarized 13CD3-pyruvate by Barb, A.W., Hekmatyar, S.K., Glushka, J.N., Prestegard, J.H.

“…[Display omitted] ▸ Deuterated 13C methyl groups prolong storage of polarization. ▸ Exchange of deuteriums for protons provide enhanced sensitivity of indirect…”
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Determination of protein backbone structures from residual dipolar couplings by Prestegard, J H, Mayer, K L, Valafar, H, Benison, G C

“…There are a number of circumstances in which a focus on determination of the backbone structure of a protein, as opposed to a complete all-atom structure, may…”
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Variation of Molecular Alignment as a Means of Resolving Orientational Ambiguities in Protein Structures from Dipolar Couplings by Al-Hashimi, H.M, Valafar, H, Terrell, M, Zartler, E.R, Eidsness, M.K, Prestegard, J.H

“…Residual dipolar couplings for pairs of proximate magnetic nuclei in macromolecules can easily be measured using high-resolution NMR methods when the molecules…”
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Exchange facilitated indirect detection of hyperpolarized 15ND2-amido-glutamine by Barb, A W, Hekmatyar, S K, Glushka, J N, Prestegard, J H

“…Hyperpolarization greatly enhances opportunities to observe in vivo metabolic processes in real time. Accessible timescales are, however, limited by nuclear…”
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Synthesis of a 15N, 13C-labeled lactam analog of a G(M4)-lactone cell-surface glycolipid by Salvatore, B.A, Prestegard, J.H

“…A 13C and 15N-labeled lactam analog of a naturally occurring G(M4) lactone glycolipid was synthesized in a convergent manner. The 13C-labels in the sialic acid…”
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Molecular Symmetry as an Aid to Geometry Determination in Ligand Protein Complexes by Al-Hashimi, H.M, Bolon, P.J, Prestegard, J.H

“…Dipole–dipole couplings between pairs of spin 12 nuclei, which can be measured from NMR spectra in field-ordered media, offer useful constraints on the…”
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Magnetically orientable phospholipid bilayers containing small amounts of a bile salt analogue, CHAPSO by Sanders, C.R., Prestegard, J.H.

“…Buffered mixtures of the detergent 3-(cholamidopropyl)dimethylammonio-2-hydroxy-1-propanesulfonate (CHAPSO) and dimyristoylphosphatidylcholine (DMPC) orient in…”
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Nuclear Magnetic Resonance Structural and Ligand Binding Studies of BLBC, a Two-Domain Fragment of Barley Lectin by Weaver, Jeanne Lim, Prestegard, J. H

“…Plant lectins are useful targets for biophysical studies of protein−carbohydrate recognition, a process of general interest because of its many roles in human…”
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Magnetic field induced ordering of bile salt/phospholipid micelles: new media for NMR structural investigations by Ram, P, Prestegard, J H

“…Micelles formed from sodium glycocholate and dimyristoylphosphatidylcholine are demonstrated to form a magnetic field orientable liquid crystal within narrow…”
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Small angle x-ray scattering studies of magnetically oriented lipid bilayers by Hare, B.J., Prestegard, J.H., Engelman, D.M.

“…Magnetically oriented lipid/detergent bilayers are potentially useful for studies of membrane-associated molecules and complexes using x-ray scattering and…”
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Backbone solution structures of proteins using residual dipolar couplings: Application to a novel structural genomics target by Valafar, H., Mayer, K. L., Bougault, C. M., LeBlond, P. D., Jenney, F. E., Brereton, P. S., Adams, M. W. W., Prestegard, J. H.

“…Structural genomics (or proteomics) activities are critically dependent on the availability of high-throughput structure determination methodology. Development…”
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Conformation of sulfoquinovosyldiacylglycerol bound to a magnetically oriented membrane system by Howard, K.P., Prestegard, J.H.

“…The conformation of uniformly 13C-labeled sulfoquinovosyldiacylglycerol (SQDG) is studied in both membrane and solution environments using NMR spectroscopy…”
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Orientational behavior of phosphatidylcholine bilayers in the presence of aromatic amphiphiles and a magnetic field by Sanders, C.R., Schaff, J.E., Prestegard, J.H.

“…A number of aromatic-containing additives which can influence the orientation of fragments of lipid bilayer membranes by a magnetic field have been…”
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Conformation of .beta.-methylmelibiose bound to the ricin B-chain as determined from transferred nuclear Overhauser effects by Bevilacqua, V. L, Kim, Yangmee, Prestegard, J. H

“…Transferred nuclear Overhauser effect (TRNOE) experiments have revealed a change in the torsion angles about the alpha-1-6 glycosidic bond of methyl…”
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