Search Results - "Predki, P F"

Effect of replacement of "zinc finger" zinc on estrogen receptor DNA interactions by Predki, P F, Sarkar, B

“…Exposure of bovine estrogen receptor to the metal chelators EDTA and 1,10-phenanthroline results in a loss of nonspecific DNA binding, presumably because of…”
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Ordered binding of retinoic acid and retinoid-X receptors to asymmetric response elements involves determinants adjacent to the DNA-binding domain by Predki, P F, Zamble, D, Sarkar, B, Giguère, V

“…Retinoic acid, a pleiotropic regulator of development and homeostasis, controls the expression of specific gene networks via direct interactions with nuclear…”
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High-throughput plasmid purification for capillary sequencing by Elkin, C J, Richardson, P M, Fourcade, H M, Hammon, N M, Pollard, M J, Predki, P F, Glavina, T, Hawkins, T L

“…The need for expeditious and inexpensive methods for high-throughput DNA sequencing has been highlighted by the accelerated pace of genome DNA sequencing over…”
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Analyzing antibody specificity with whole proteome microarrays by Schweitzer, Barry I, Michaud, Gregory A, Salcius, Michael, Zhou, Fang, Bangham, Rhonda, Bonin, Jaclyn, Guo, Hong, Snyder, Michael, Predki, Paul F

“…Although approximately 10,000 antibodies are available from commercial sources, antibody reagents are still unavailable for most proteins. Furthermore, new…”
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Functional protein microarrays: ripe for discovery by Predki, Paul F

“…The manufacture and use of protein microarrays with correctly folded and functional content presents significant challenges. Despite this, the feasibility and…”
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Further characterization of the N-terminal copper(II)- and nickel(II)-binding motif of proteins : studies of metal binding to chicken serum albumin and the native sequence peptide by PREDKI, P. F, HARFORD, C, BRAR, P, SARKAR, B

“…We have investigated the Cu(II)- and Ni(II)-binding properties of chicken serum albumin (CSA) and of the native sequence tripeptide derived from the N-terminus…”
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Metal Replacement in "Zinc Finger" and Its Effect on DNA Binding by Predki, Paul F., Sarkar, Bibudhendra

“…Metal replacement studies were used to investigate the metal requirement of a bacterially expressed polypeptide encoding the zinc finger DNA binding domain of…”
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Characterization and cellular distribution of acidic peptide and oligosaccharide metal-binding compounds from kidneys by PREDKI, P. F, WHITFIELD, D. M, SARKAR, B

“…Two low-molecular-mass Ni-binding fractions first isolated from human kidneys [Templeton & Sarkar (1985) Biochem. J. 230, 35-42] are further characterized…”
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Gene expression analysis by transcript profiling coupled to a gene database query by Shimkets, Richard A, Lowe, David G, Tai, Julie Tsu-Ning, Sehl, Patricia, Jin, Hongkui, Yang, Renhui, Predki, Paul F, Rothberg, Bonnie E. G, Murtha, Michael T, Roth, Matthew E, Shenoy, Suresh G, Windemuth, Andreas, Simpson, John W, Simons, Jan F, Daley, Michael P, Gold, Steven A, McKenna, Michael P, Hillan, Kenneth, Went, Gregory T, Rothberg, Jonathan M

“…We describe an mRNA profiling technique for determining differential gene expression that utilizes, but does not require, prior knowledge of gene sequences…”
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Cooperative interaction of oestrogen receptor 'zinc finger' domain polypeptides on DNA binding by Predki, P F, Sarkar, B

“…The consensus oestrogen response element (ERE) contains two inverted copies of an AGGTCA consensus hexameric half-site, spaced by three base pairs. It differs…”
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Whole-Genome Comparative Analysis of Three Phytopathogenic Xylella fastidiosa Strains by Bhattacharyya, Anamitra, Stilwagen, Stephanie, Ivanova, Natalia, D'Souza, Mark, Bernal, Axel, Lykidis, Athanasios, Kapatral, Vinayak, Anderson, Iain, Larsen, Niels, Los, Tamara, Reznik, Gary, Selkov, Eugene, Walunas, Theresa L., Feil, Helene, Feil, William S., Purcell, Alexander, Lassez, Jean-Louis, Hawkins, Trevor L., Haselkorn, Robert, Overbeek, Ross, Predki, Paul F., Kyrpides, Nikos C.

“…Xylella fastidiosa (Xf) causes wilt disease in plants and is responsible for major economic and crop losses globally. Owing to the public importance of this…”
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The home stretch, a first analysis of the nearly completed genome of Rhodobacter sphaeroides 2.4.1 by Mackenzie, C, Choudhary, M, Larimer, F W, Predki, P F, Stilwagen, S, Armitage, J P, Barber, R D, Donohue, T J, Hosler, J P, Newman, J E, Shapleigh, J P, Sockett, R E, Zeilstra-Ryalls, J, Kaplan, S

“…Rhodobacter sphaeroides 2.4.1 is an alpha-3 purple nonsulfur eubacterium with an extensive metabolic repertoire. Under anaerobic conditions, it is able to grow…”
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Dissecting RNA-protein interactions: RNA-RNA recognition by Rop by Predki, Paul F, Mike Nayak, L, Gottlieb, Morris B.C, Regan, Lynne

“…The ColE1 plasmid of E. coli encodes a small RNA-binding protein, Rop, which is involved in the regulation of plasmid copy number. Rop, a 4-helix bundle…”
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Development of functional protein microarrays for drug discovery: progress and challenges by Zhou, Fang X, Bonin, Jaclyn, Predki, Paul F

“…Functional protein microarrays promise new approaches to address longstanding challenges in drug discovery and development, with applications ranging from…”
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ColE1-compatible vectors for high-level expression of cloned DNAs from the T7 promoter by Munson, M, Predki, P F, Regan, L

“…A new family of T7-based expression plasmids with unique features is described. The plasmid origin of replication (ori), derived from P15A, is compatible with…”
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Amino-acid substitutions in a surface turn modulate protein stability by Predki, Paul F, Agrawal, Vishal, Brünger, Axel T, Regan, Lynne

“…A surface turn position in a four-helix bundle protein, Rop, was selected to investigate the role of turns in protein structure and stability. Although all…”
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Global analysis of protein phosphorylation in yeast by Ptacek, J, Devgan, G, Michaud, G, Zhu, H, Zhu, X, Fasolo, J, Guo, H, Jona, G, Breitkreutz, A, Sopko, R

“…Protein phosphorylation is estimated to affect 30% of the proteome and is a major regulatory mechanism that controls many basic cellular processes. Until…”
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Redesigning the Topology of a Four-Helix-Bundle Protein: Monomeric Rop by Predki, Paul F, Regan, Lynne

“…The topology of alpha-helices and beta-sheets in folded proteins is largely specified by the connectivities of the loops and turns which join them. We have…”
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Functional protein microarrays: just how functional are they? by Merkel, Janie S, Michaud, Gregory A, Salcius, Michael, Schweitzer, Barry, Predki, Paul F

“…Arrays of immobilized proteins have been developed for the discovery and characterization of protein functions ranging from molecular recognition to enzymatic…”
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A pooling-deconvolution strategy for biological network elucidation by Predki, Paul F, Fields, Stanley, Huang, Jing, Jin, Fulai, Hazbun, Tony, Michaud, Gregory A, Salcius, Michael

“…The generation of large-scale data sets is a fundamental requirement of systems biology. But despite recent advances, generation of such high-coverage data…”
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