Stromal remodelling is required for progressive involution of the rat ventral prostate after castration: Identification of a matrix metalloproteinase-dependent apoptotic wave

Prostate epithelial-cell apoptosis occurs in response to androgen deprivation. We have hypothesized that continued regression would require stromal changes. Studying apoptosis kinetics up to the 14th day after castration, we identified successive waves of apoptosis, with a prominent peak on day 11....

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Bibliographic Details
Published in:	International journal of andrology Vol. 33; no. 5; pp. 686 - 695
Main Authors:	Bruni-Cardoso, A, Augusto, T.M, Pravatta, H, Damas-Souza, D.M, Carvalho, H.F
Format:	Journal Article
Language:	English
Published:	Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01-10-2010 Blackwell Publishing Ltd Blackwell
Subjects:	Androgen Antagonists - pharmacology Androgens - physiology Animals apoptosis Apoptosis - drug effects Biological and medical sciences castration Dipeptides - pharmacology Doxycycline - pharmacology Extracellular Matrix - metabolism Fundamental and applied biological sciences. Psychology GM6001 Gynecology. Andrology. Obstetrics Hydrocortisone - pharmacology Male Male genital diseases Mammalian male genital system Matrix Metalloproteinase Inhibitors matrix metalloproteinases Matrix Metalloproteinases - metabolism Medical sciences Orchiectomy prostate Prostate - pathology Rats Rats, Wistar Stromal Cells - pathology tissue remodelling Vertebrates: reproduction Rat castration Rodentia Male genital system Vertebrata Involution Reproduction Mammalia tissue remodelling Cell death Animal Metalloproteinase Urogenital system Prostate matrix metalloproteinases Apoptosis GM6001
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Summary:	Prostate epithelial-cell apoptosis occurs in response to androgen deprivation. We have hypothesized that continued regression would require stromal changes. Studying apoptosis kinetics up to the 14th day after castration, we identified successive waves of apoptosis, with a prominent peak on day 11. This peak was associated with caspase-3 activity, nuclear translocation of apoptosis-inducing factor and clusterin expression. The apoptosis peak on day 11 was preceded by increased MMP-2 and MMP-7 activation, and MMP-9 expression on days 9 and 10. Treatment with the matrix metalloproteinases inhibitors doxycyclin, hydrocortisone, or GM6001 caused significant reduction in the apoptosis rate on day 11. The present data demonstrate that prostatic epithelial-cell deletion at the 11th day after castration was induced by focal degradation of the extracellular matrix associated with stromal remodelling.
Bibliography:	http://dx.doi.org/10.1111/j.1365-2605.2009.01004.x ArticleID:IJAN1004 istex:4A49F05D0328D83F0FD7CAED6627928EE761B063 ark:/67375/WNG-S3D4HL85-9 These authors contributed equally to this work ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0105-6263 1365-2605
DOI:	10.1111/j.1365-2605.2009.01004.x