Antarctic ice core samples: culturable bacterial diversity

Culturable bacterial abundance at 11 different depths of a 50.26 m ice core from the Tallaksenvarden Nunatak, Antarctica, varied from 0.02 to 5.8 × 103 CFU ml−1 of the melt water. A total of 138 bacterial strains were recovered from the 11 different depths of the ice core. Based on 16S rRNA gene seq...

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Bibliographic Details
Published in:	Research in microbiology Vol. 164; no. 1; pp. 70 - 82
Main Authors:	Shivaji, Sisinthy, Begum, Zareena, Shiva Nageswara Rao, Singireesu Soma, Vishnu Vardhan Reddy, Puram V., Manasa, Poorna, Sailaja, Buddi, Prathiba, Mambatta S., Thamban, Meloth, Krishnan, Kottekkatu P., Singh, Shiv M., Srinivas, Tanuku N.R.
Format:	Journal Article
Language:	English
Published:	France Elsevier Masson SAS 01-01-2013
Subjects:	16S rRNA gene Actinobacteria Adaptation, Physiological Antarctic Regions Bacteria - classification Bacteria - genetics Bacteria - isolation & purification Bacteria - metabolism Bacterial Load Bacteroidetes Biodiversity Enzymes - metabolism Extracellular enzymatic activities Fatty acid profiles Fatty Acids Firmicutes Freezing Ice - analysis Molecular Sequence Data Phenotypic characteristics Phylogeny Proteobacteria RNA, Ribosomal, 16S - genetics Water Microbiology Antarctica Antarctic region Antarctic Regions Proteobacteria Extracellular enzymatic activities Phenotypic characteristics Fatty acid profiles Actinobacteria Bacteroidetes 16S rRNA gene Firmicutes
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Summary:	Culturable bacterial abundance at 11 different depths of a 50.26 m ice core from the Tallaksenvarden Nunatak, Antarctica, varied from 0.02 to 5.8 × 103 CFU ml−1 of the melt water. A total of 138 bacterial strains were recovered from the 11 different depths of the ice core. Based on 16S rRNA gene sequence analyses, the 138 isolates could be categorized into 25 phylotypes belonging to phyla Actinobacteria, Bacteroidetes, Firmicutes and Proteobacteria. All isolates had 16S rRNA sequences similar to previously determined sequences (97.2–100%). No correlation was observed in the distribution of the isolates at the various depths either at the phylum, genus or species level. The 25 phylotypes varied in growth temperature range, tolerance to NaCl, growth pH range and ability to produce eight different extracellular enzymes at either 4 or 18 °C. Iso-, anteiso-, unsaturated and saturated fatty acids together constituted a significant proportion of the total fatty acid composition.
Bibliography:	http://dx.doi.org/10.1016/j.resmic.2012.09.001 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0923-2508 1769-7123
DOI:	10.1016/j.resmic.2012.09.001