Restoring Sperm Quality Post-Cryopreservation Using Mitochondrial-Targeted Compounds

Restoring Sperm Quality Post-Cryopreservation Using Mitochondrial-Targeted Compounds

While critical for male fertility preservation, cryopreservation damage reduces sperm quality and fertilization potential. This study investigated whether the addition of mitochondrial-targeted, antioxidant compounds, also known as Mitochondrial activators, to the cryopreservation medium could prote...

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Bibliographic Details
Published in:Antioxidants Vol. 11; no. 9; p. 1808
Main Authors: Gonzalez, Macarena, Prashar, Tanisha, Connaughton, Haley, Barry, Michael, Robker, Rebecca, Rose, Ryan
Format: Journal Article
Language:English
Published: Basel MDPI AG 01-09-2022
MDPI
Subjects:
Antioxidants
Carnitine
Consent
Cryopreservation
Deoxyribonucleic acid
DNA
DNA damage
DNA fragmentation
Fertility
Fertilization
In vitro fertilization
L-Carnitine
Lipid peroxidation
Membrane potential
Mitochondria
Motility
Oxidation
Oxidative stress
Physiological aspects
Reactive oxygen species
Reproductive technologies
Semen
Sperm
Spermatozoa
Thawing
Viability
United States
United States > US
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Summary:While critical for male fertility preservation, cryopreservation damage reduces sperm quality and fertilization potential. This study investigated whether the addition of mitochondrial-targeted, antioxidant compounds, also known as Mitochondrial activators, to the cryopreservation medium could protect sperm quality during cryopreservation. For this, semen samples from men undergoing IVF/ICSI treatment, which were donated for research, underwent cryopreservation in the absence or presence of BGP-15, MitoQ and L-carnitine. Fresh semen and thawed sperm samples from the same participant were analyzed for indicators of sperm quality: sperm viability, kinetics, mitochondrial reactive oxygen species (ROS) levels, Mitochondrial Membrane Potential (MMP) and DNA damage. Cryopreservation significantly reduced sperm viability and motility and predicted mucous penetration. BGP-15, MitoQ and L-carnitine improved sperm motility, whilst the addition of L-Carnitine prevented the loss of sperm viability during cryopreservation. Both BGP-15 and L-carnitine reduced sperm DNA oxidative damage, but only BGP-15 significantly reduced DNA fragmentation. More importantly, BGP-15 increased sperm predictive mucous penetration and MMP and reduced DNA oxidation. Our results show that the addition of BGP-15 or L-carnitine to the cryopreservation medium improves sperm quality post-thawing, highlighting the potential of mitochondrial antioxidants to improve long-term fertility preservation in males.
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ISSN:2076-3921
2076-3921
DOI:10.3390/antiox11091808