Biodegradation of trichloroethylene (TCE) by methanotrophic community

Biodegradation of trichloroethylene (TCE) by methanotrophic community

Laboratory incubation experiments were carried out to assess the potential of methanotrophic culture for degrading TCE. Measurements of the growth rate and TCE degradation showed that the methanotrophs not only grew in presence of TCE but also degraded TCE. The rate of TCE degradation was found to b...

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Bibliographic Details
Published in:Bioresource technology Vol. 100; no. 9; pp. 2469 - 2474
Main Authors: Shukla, Awadhesh K., Vishwakarma, Pranjali, Upadhyay, S.N., Tripathi, Anil K, Prasana, H.C., Dubey, Suresh K.
Format: Journal Article
Language:English
Published: Kidlington Elsevier Ltd 01-05-2009
Elsevier
Subjects:
Biodegradation, Environmental
Biodiversity
Biological and medical sciences
Bioremediation
Biotechnology
Environment and pollution
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Industrial applications and implications. Economical aspects
Methanotrophs
Methylocystaceae - classification
Methylocystaceae - genetics
Methylocystaceae - metabolism
Methylocystis
Molecular Sequence Data
Phylogeny
pMMO
Polymorphism, Restriction Fragment Length
Reverse Transcriptase Polymerase Chain Reaction
RT–PCR
TCE
Trichloroethylene - metabolism
pMMO
Bioremediation
RT–PCR
Methanotrophs
TCE
Biodegradation
Methanotrophy
Pollution control
RT-PCR
Reverse transcription polymerase chain reaction
Microbial community
Ethylene(trichloro)
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Summary:Laboratory incubation experiments were carried out to assess the potential of methanotrophic culture for degrading TCE. Measurements of the growth rate and TCE degradation showed that the methanotrophs not only grew in presence of TCE but also degraded TCE. The rate of TCE degradation was found to be 0.19 ppm h −1. The reverse transcriptase–PCR test was conducted to quantify expression of pmoA and mmoX genes. RT–PCR revealed expression of pmoA gene only. This observation provides evidence that the pmoA gene was functionally active for pMMO enzyme during the study. The diversity of the methanotrophs involved in TCE degradation was assessed by PCR amplification, cloning, restriction fragment length polymorphism and phylogenetic analysis of pmoA genes. Results suggested the occurrence of nine different phylotypes belonging to Type II methanotrophs in the enriched cultures. Out of the nine, five clustered with, genera Methylocystis and rest got clustered in to a separate group.
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ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2008.12.022