Uniform aligned bioconjugation of biomolecule motifs for integration within microfabricated microfluidic devices

Full details and a step-by-step guide suitable for printing proteins aligned to micron-sized sensors and subsequent integration and alignment of microfluidic structures are presented. The precise alignment and grafting of micron-sized biomolecule patterns with an underlying substrate at predefined l...

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Bibliographic Details
Published in:	Analytical biochemistry Vol. 424; no. 2; pp. 195 - 205
Main Authors:	Braddick, Lucy M., Garland, Patrick J., Praeger, Matthew F., Butement, Jonathan, Friedrich, Daniel, Morgan, David J., Melvin, Tracy
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 15-05-2012
Subjects:	Antibodies - chemistry Avidin - chemistry Bioaffinity sensors Bioconjugation Biosensing Techniques - instrumentation Biosensing Techniques - methods Biosensors Biotin - chemistry Carbocyanines Cytokines - blood denaturation Dextrans - chemistry Humans integration Lab-on-a-chip Lab-On-A-Chip Devices Microcontact printing Microfabrication Microfluidic Microfluidic Analytical Techniques - instrumentation Microfluidic Analytical Techniques - methods Microtechnology Photoelectron Spectroscopy proteins reproduction silicon Silicon Dioxide - chemistry Tumor Necrosis Factor-alpha - chemistry Bioaffinity sensors Microcontact printing Microfabrication Microfluidic Lab-on-a-chip Bioconjugation Biosensors
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Summary:	Full details and a step-by-step guide suitable for printing proteins aligned to micron-sized sensors and subsequent integration and alignment of microfluidic structures are presented. The precise alignment and grafting of micron-sized biomolecule patterns with an underlying substrate at predefined locations is achieved using a novel semi-automated microcontact printer. Through integration of optical alignment methods in the x, y, and z directions, uniform contact of micron-sized stamps is achieved. Feature compression of the stamp is avoided by fine control of the stamp during contact. This printing method has been developed in combination with robust, compatible bioconjugate chemistry for patterning of a dextran-functionalized silicon oxide substrate with a NeutrAvidin-“inked” stamp and subsequent incubation with a biotin-functionalized protein. The bioconjugate chemistry is such that uniform coverage of the protein (without denaturation) over the printed motif is obtained and reproduction of the initial mask shape and dimensions is achieved. Later integration with a microfluidic structure aligned with the printed motif on the substrate is also described.
Bibliography:	http://dx.doi.org/10.1016/j.ab.2012.02.020 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0003-2697 1096-0309
DOI:	10.1016/j.ab.2012.02.020