Correlation between indoleamine 2,3 dioxygenase mRNA and CDKN2A/p16 mRNA: a combined strategy to cervical cancer diagnosis

Cervical cancer (CC) is one of the most common cancers among women worldwide. The relation of the human papillomavirus (HPV) with CC and its precursor lesions was first suspected for over 40 years. The indoleamine 2,3 dioxygenase (IDO) is an immune modulator enzyme responsible for the immune system...

Full description

Saved in:

Bibliographic Details
Published in:	Medical oncology (Northwood, London, England) Vol. 33; no. 11; p. 132
Main Authors:	Saffi Junior, Mario Cezar, Duarte, Ivone da Silva, Brito, Rodrigo Barbosa de Oliveira, Prado, Giovana Garcia, Makabe, Sergio, Dellê, Humberto, Camacho, Cleber P.
Format:	Journal Article
Language:	English
Published:	New York Springer US 01-11-2016 Springer Nature B.V
Subjects:	Adolescent Adult Aged Cervical cancer Cyclin-Dependent Kinase Inhibitor p18 - genetics Female Gene Expression Regulation, Neoplastic Hematology Humans Indoleamine-Pyrrole 2,3,-Dioxygenase - genetics Internal Medicine Medicine Medicine & Public Health Middle Aged Oncology Original Paper Papillomavirus E7 Proteins - genetics Pathology RNA, Messenger Uterine Cervical Neoplasms - diagnosis Uterine Cervical Neoplasms - genetics Uterine Cervical Neoplasms - pathology Young Adult mRNA Screening program IDO CDKN2A Cervical cancer Cytology
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Cervical cancer (CC) is one of the most common cancers among women worldwide. The relation of the human papillomavirus (HPV) with CC and its precursor lesions was first suspected for over 40 years. The indoleamine 2,3 dioxygenase (IDO) is an immune modulator enzyme responsible for the immune system tissue protection mechanism, which may be the key to the tumoural persistence. HPV oncoprotein E7 promotes the increase in cyclin-dependent kinase inhibitor p16 (CDKN2A/p16). The isolated and combined analysis of CDKN2A/p16 mRNA to CC diagnosis was done with promising results. The aim of this study is to evaluate the correlation between IDO mRNA and CDKN2A/p16 mRNA. We will explore the potential of both as diagnostic tools. RNA was extracted from tissue samples. cDNA was generated with High Capacity RNA-to-cDNA kit. The real-time PCR results were analysed using nonlinear curve estimation, ROC curve, Chi-squared test, the proportion of variance explained and Galen and Gambino formulas. From 270 patients attended, colposcopy examination was performed in 110 and the biopsy in 75 patients. We found a positive correlation in patients older than 28 years old with low-risk lesions, but the correlation is lost in high-risk lesions. Although cytology, IDO mRNA and CDKN2A/p16 mRNA could not differentiate the risk groups, IDO combined with CDKN2A/p16 mRNA results could ( p = 0.028). The best diagnostic result was achieved by IDO coupled with CDKN2A/p16 mRNA, which may considerably increase the sensitivity of screening for CC.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1357-0560 1559-131X
DOI:	10.1007/s12032-016-0844-7