Codon adaptation-based control of protein expression in C. elegans

We present a method to control protein levels under native genetic regulation in Caenorhabditis elegans by using synthetic genes with adapted codons. We found that the force acting on the spindle in C. elegans embryos was related to the amount of the G-protein regulator GPR-1/2. Codon-adapted versio...

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Bibliographic Details
Published in:	Nature methods Vol. 8; no. 3; pp. 250 - 252
Main Authors:	Bringmann, Henrik, Redemann, Stefanie, Schloissnig, Siegfried, Ernst, Susanne, Pozniakowsky, Andrey, Ayloo, Swathi, Hyman, Antony A
Format:	Journal Article
Language:	English
Published:	New York Nature Publishing Group US 01-03-2011 Nature Publishing Group
Subjects:	631/1647/338/552 631/208/200 Animals Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology brief-communication Caenorhabditis elegans Caenorhabditis elegans - genetics Caenorhabditis elegans Proteins - genetics Cell Division - genetics Codon Codon - genetics Embryos Gene expression Gene Expression Regulation Genetic aspects Genetic regulation Genetic Techniques Life Sciences Nematodes Physiological aspects Protein Biosynthesis - genetics Protein research Proteins Proteomics Scientific method United States
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Summary:	We present a method to control protein levels under native genetic regulation in Caenorhabditis elegans by using synthetic genes with adapted codons. We found that the force acting on the spindle in C. elegans embryos was related to the amount of the G-protein regulator GPR-1/2. Codon-adapted versions of any C. elegans gene can be designed using our web tool, C. elegans codon adapter.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1548-7091 1548-7105
DOI:	10.1038/nmeth.1565