Search Results - "Poyner, David"

Update on the pharmacology of calcitonin/CGRP family of peptides: IUPHAR Review 25 by Hay, Debbie L, Garelja, Michael L, Poyner, David R, Walker, Christopher S

“…The calcitonin/CGRP family of peptides includes calcitonin, α and β CGRP, amylin, adrenomedullin (AM) and adrenomedullin 2/intermedin (AM2/IMD). Their…”
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A comparison of SMA (styrene maleic acid) and DIBMA (di-isobutylene maleic acid) for membrane protein purification by Gulamhussein, Aiman A., Uddin, Romez, Tighe, Brian J., Poyner, David R., Rothnie, Alice J.

“…The use of styrene maleic acid co-polymer (SMA) for membrane protein extraction and purification has grown in recent years. SMA inserts in the membrane and…”
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The pharmacology of Adrenomedullin 2/Intermedin by Hong, Yanguo, Hay, Debbie L, Quirion, Remi, Poyner, David R

“…Adrenomedullin 2 (AM2) or intermedin is a member of the calcitonin gene‐related peptide (CGRP)/calcitonin family of peptides and was discovered in 2004. Unlike…”
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Structural Basis for Receptor Activity-Modifying Protein-Dependent Selective Peptide Recognition by a G Protein-Coupled Receptor by Booe, Jason M., Walker, Christopher S., Barwell, James, Kuteyi, Gabriel, Simms, John, Jamaluddin, Muhammad A., Warner, Margaret L., Bill, Roslyn M., Harris, Paul W., Brimble, Margaret A., Poyner, David R., Hay, Debbie L., Pioszak, Augen A.

“…Association of receptor activity-modifying proteins (RAMP1-3) with the G protein-coupled receptor (GPCR) calcitonin receptor-like receptor (CLR) enables…”
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G-protein coupled receptor solubilization and purification for biophysical analysis and functional studies, in the total absence of detergent by Jamshad, Mohammed, Charlton, Jack, Lin, Yu-Pin, Routledge, Sarah J, Bawa, Zharain, Knowles, Timothy J, Overduin, Michael, Dekker, Niek, Dafforn, Tim R, Bill, Roslyn M, Poyner, David R, Wheatley, Mark

“…G-protein coupled receptors (GPCRs) constitute the largest class of membrane proteins and are a major drug target. A serious obstacle to studying GPCR…”
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Microbial expression systems for membrane proteins by Dilworth, Marvin V., Piel, Mathilde S., Bettaney, Kim E., Ma, Pikyee, Luo, Ji, Sharples, David, Poyner, David R., Gross, Stephane R., Moncoq, Karine, Henderson, Peter J.F., Miroux, Bruno, Bill, Roslyn M.

“…•Microbes have generated 71% of recombinant membrane proteins with unique structures.•Data on usage of strains, tags and promoters are discussed.•Detergents…”
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Receptor Activity-modifying Protein-directed G Protein Signaling Specificity for the Calcitonin Gene-related Peptide Family of Receptors by Weston, Cathryn, Winfield, Ian, Harris, Matthew, Hodgson, Rose, Shah, Archna, Dowell, Simon J, Mobarec, Juan Carlos, Woodlock, David A, Reynolds, Christopher A, Poyner, David R, Watkins, Harriet A, Ladds, Graham

“…The calcitonin gene-related peptide (CGRP) family of G protein-coupled receptors (GPCRs) is formed through the association of the calcitonin receptor-like…”
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Single molecule binding of a ligand to a G-protein-coupled receptor in real time using fluorescence correlation spectroscopy, rendered possible by nano-encapsulation in styrene maleic acid lipid particles by Grime, Rachael L, Goulding, Joelle, Uddin, Romez, Stoddart, Leigh A, Hill, Stephen J, Poyner, David R, Briddon, Stephen J, Wheatley, Mark

“…The fundamental importance of membrane proteins in cellular processes has driven a marked increase in the use of membrane mimetic approaches for studying and…”
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Receptor activity-modifying proteins; multifunctional G protein-coupled receptor accessory proteins by Hay, Debbie L, Walker, Christopher S, Gingell, Joseph J, Ladds, Graham, Reynolds, Christopher A, Poyner, David R

“…Receptor activity-modifying proteins (RAMPs) are single pass membrane proteins initially identified by their ability to determine the pharmacology of the…”
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Calcitonin Receptor N-Glycosylation Enhances Peptide Hormone Affinity by Controlling Receptor Dynamics by Lee, Sang-Min, Jeong, Yejin, Simms, John, Warner, Margaret L., Poyner, David R., Chung, Ka Young, Pioszak, Augen A.

“…The class B G protein-coupled receptor (GPCR) calcitonin receptor (CTR) is a drug target for osteoporosis and diabetes. N-glycosylation of asparagine 130 in…”
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Regulation of signal transduction by calcitonin gene-related peptide receptors by Walker, Christopher S, Conner, Alex C, Poyner, David R, Hay, Debbie L

“…Calcitonin gene-related peptide (CGRP) plays a pivotal role in migraine, activating its cognate receptor to initiate intracellular signalling. This atypical…”
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GPCRs in the round: SMA-like copolymers and SMALPs as a platform for investigating GPCRs by Ayub, Hoor, Murray, Rebecca J., Kuyler, Gestél C., Napier-Khwaja, Farhaan, Gunner, Joseph, Dafforn, Tim R., Klumperman, Bert, Poyner, David R., Wheatley, Mark

“…G-protein-coupled receptors (GPCRs) are the largest family of membrane proteins, regulate a plethora of physiological responses and are the therapeutic target…”
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Expression and purification of recombinant G protein-coupled receptors: A review by Wiseman, Daniel N., Otchere, Abigail, Patel, Jaimin H., Uddin, Romez, Pollock, Naomi L., Routledge, Sarah J., Rothnie, Alice J., Slack, Cathy, Poyner, David R., Bill, Roslyn M., Goddard, Alan D.

“…Given their extensive role in cell signalling, GPCRs are significant drug targets; despite this, many of these receptors have limited or no available…”
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Extracellular loops 1 and 3 and their associated transmembrane regions of the calcitonin receptor-like receptor are needed for CGRP receptor function by Barwell, James, Conner, Alex, Poyner, David R.

“…The first and third extracellular loops (ECL) of G protein-coupled receptors (GPCRs) have been implicated in ligand binding and receptor function. This study…”
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The effects of RAMPs upon cell signalling by Routledge, Sarah J., Ladds, Graham, Poyner, David R.

“…G protein-coupled receptors (GPCRs) play a vital role in signal transduction. It is now clear that numerous other molecules within the cell and at the cell…”
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Intracellular pathways of calcitonin gene‐related peptide‐induced relaxation of human coronary arteries: A key role for Gβγ subunit instead of cAMP by Vries, Tessa, Labruijere, Sieneke, Rivera‐Mancilla, Eduardo, Garrelds, Ingrid M., Vries, René, Schutter, Dennis, Bogaerdt, Antoon, Poyner, David R., Ladds, Graham, Danser, A. H. Jan, MaassenVanDenBrink, Antoinette

“…Background and Purpose Calcitonin gene‐related peptide (CGRP) is a potent vasodilator. While its signalling is assumed to be mediated via increases in cAMP,…”
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GPCR-styrene maleic acid lipid particles (GPCR-SMALPs): their nature and potential by Wheatley, Mark, Charlton, Jack, Jamshad, Mohammed, Routledge, Sarah J, Bailey, Sian, La-Borde, Penelope J, Azam, Maria T, Logan, Richard T, Bill, Roslyn M, Dafforn, Tim R, Poyner, David R

“…G-protein-coupled receptors (GPCRs) form the largest class of membrane proteins and are an important target for therapeutic drugs. These receptors are highly…”
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Ligand-induced conformational changes in a SMALP-encapsulated GPCR by Routledge, Sarah J., Jamshad, Mohammed, Little, Haydn A., Lin, Yu-Pin, Simms, John, Thakker, Alpesh, Spickett, Corinne M., Bill, Roslyn M., Dafforn, Tim R., Poyner, David R., Wheatley, Mark

“…The adenosine 2A receptor (A2AR), a G-protein-coupled receptor (GPCR), was solubilised and purified encapsulated in styrene maleic acid lipid particles…”
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Determining the Effects of Differential Expression of GRKs and β-arrestins on CLR-RAMP Agonist Bias by Pearce, Abigail, Redfern-Nichols, Theo, Harris, Matthew, Poyner, David R, Wigglesworth, Mark, Ladds, Graham

“…Signalling of the calcitonin-like receptor (CLR) is multifaceted, due to its interaction with receptor activity modifying proteins (RAMPs), and three…”
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Differences in SMA-like polymer architecture dictate the conformational changes exhibited by the membrane protein rhodopsin encapsulated in lipid nano-particles by Grime, Rachael L, Logan, Richard T, Nestorow, Stephanie A, Sridhar, Pooja, Edwards, Patricia C, Tate, Christopher G, Klumperman, Bert, Dafforn, Tim R, Poyner, David R, Reeves, Philip J, Wheatley, Mark

“…Membrane proteins are of fundamental importance to cellular processes and nano-encapsulation strategies that preserve their native lipid bilayer environment…”
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