Dual-energy X-ray absorptiometry of the pig: Protocol development and evaluation

Dual-energy X-ray absorptiometry of the pig: Protocol development and evaluation

As part of a prospective study in bone mineralisation in adult pigs it was necessary to establish guidelines and to define sites for bone mineral measurements. Particular requirements were that, the protocol should be suitable for a mass screening programme in both postmortem specimens and in live a...

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Bibliographic Details
Published in:Meat science Vol. 68; no. 2; pp. 235 - 241
Main Authors: Nielsen, D.H, McEvoy, F.J, Poulsen, H.L, Madsen, M.T, Buelund, L.E, Svalastoga, E
Format: Journal Article
Language:English
Published: Oxford Elsevier Ltd 01-10-2004
Elsevier
Subjects:
Accuracy
Animal productions
Biological and medical sciences
bone density
Bone mineralisation
bone mineralization
calcium
dietary minerals
dual-energy X-ray absorptiometry
feeds
Food industries
Fundamental and applied biological sciences. Psychology
measurement
Meat and meat product industries
nutrient content
nutrient deficiencies
phosphorus
Pig
Precision
swine
swine feeding
Terrestrial animal productions
Vertebrates
Accuracy
Precision
Bone mineralisation
Pig
Vertebrata
Meat animals
Farming animal
Mammalia
Development
Meat product
Artiodactyla
Ungulata
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Summary:As part of a prospective study in bone mineralisation in adult pigs it was necessary to establish guidelines and to define sites for bone mineral measurements. Particular requirements were that, the protocol should be suitable for a mass screening programme in both postmortem specimens and in live animals, and should deliver results of known reliability. Estimates of bone mineral content (BMC) and bone mineral density (BMD) in areas within the 4th metacarpal bone yielded coefficients of variation (CV) in the order of 7% for both regions and estimates in regions which included the entire metacarpal–phalangeal area yielded CV values in the order of 0.7% and 0.6% for BMC and BMD, respectively. A region of interest taken from the coccygeal vertebrae yielded coefficient of variation values of 3% and 2% for BMC and BMD, respectively. Accuracy of dual-energy X-ray absorptiometry (DXA) was estimated using a standard curve derived from BMC determined by ashing. There was a high correlation between mineral content determined by DXA and by ashing ( R 2=0.99, p<0.0001). The results suggest that the regions used in this study are suitable for use in large, mass screening, prospective studies.
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ISSN:0309-1740
1873-4138
DOI:10.1016/j.meatsci.2004.03.002