Growth factor-functionalized silk membranes support wound healing in vitro

Growth factor-functionalized silk membranes support wound healing in vitro

Chronic wounds represent a serious problem in daily medical routine requiring improved wound care. Silk of the domesticated silkworm (Bombyx mori) has been used to form a variety of biomaterials for medical applications. We genetically engineered B. mori to produce silk functionalized with growth fa...

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Bibliographic Details
Published in:Biomedical materials (Bristol) Vol. 12; no. 4; p. 045023
Main Authors: Bienert, Michaela, Hoss, M, Bartneck, M, Weinandy, S, Böbel, M, Jockenhövel, S, Knüchel, R, Pottbacker, K, Wöltje, M, Jahnen-Dechent, W, Neuss, S
Format: Journal Article
Language:English
Published: England 16-08-2017
Subjects:
Animals
Animals, Genetically Modified
Biocompatible Materials - chemistry
Bombyx - genetics
Cell Line
Humans
In Vitro Techniques
Intercellular Signaling Peptides and Proteins - administration & dosage
Intercellular Signaling Peptides and Proteins - genetics
Macrophages - drug effects
Macrophages - physiology
Materials Testing
Mice
Neovascularization, Physiologic - drug effects
Recombinant Proteins - administration & dosage
Recombinant Proteins - genetics
Silk - chemistry
Skin - drug effects
Skin - injuries
Tissue Scaffolds
Wound Healing - drug effects
Wound Healing - physiology
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Summary:Chronic wounds represent a serious problem in daily medical routine requiring improved wound care. Silk of the domesticated silkworm (Bombyx mori) has been used to form a variety of biomaterials for medical applications. We genetically engineered B. mori to produce silk functionalized with growth factors to promote wound healing in vitro. In this study FGF-, EGF-, KGF-, PDGF- or VEGF-functionalized silk membranes were compared to native B. mori silk membranes without growth factors for their ability to support wound healing in vitro. All silk membranes were cytocompatible and supported macrophage secretion of neutrophil recruiting factor CXCL1 and monocyte chemoattractant protein 1 (MCP-1). VEGF-functionalized silk significantly outperformed other growth factor-functionalized silk membranes, but not native silk in angiogenesis assays. In addition, EGF- and VEGF-functionalized silk membranes slightly enhanced macrophage adhesion compared to silk without growth factors. In wound healing assays in vitro (reduction of wound lesion), dermal equivalents showed a higher wound healing capacity when covered with EGF-, FGF- or VEGF-functionalized silk membranes compared to native, KGF- or PDGF-functionalized silk membranes. Keratinocyte migration and growth is overstimulated by KGF- and VEGF-functionalized silk membranes. In conclusion, growth factor-functionalized silk membranes prepared from genetically engineered silk worm glands are promising wound dressings for future wound healing therapies.
ISSN:1748-605X
DOI:10.1088/1748-605X/aa7695