Search Results - "Pospíšil, Jakub"
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Highly compact and cost-effective 2-beam super-resolution structured illumination microscope based on all-fiber optic components
Published in Optics express (12-04-2021)“…Current super-resolution structured illumination microscopes (SR-SIM) utilize relatively expensive electro-optic components and free-space optics, resulting in…”
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Journal Article -
2
Structure and replication of Pseudomonas aeruginosa phage JBD30
Published in The EMBO journal (01-10-2024)“…Bacteriophages are the most abundant biological entities on Earth, but our understanding of many aspects of their lifecycles is still incomplete. Here, we have…”
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Journal Article -
3
In-section Click-iT detection and super-resolution CLEM analysis of nucleolar ultrastructure and replication in plants
Published in Nature communications (19-03-2024)“…Correlative light and electron microscopy (CLEM) is an important tool for the localisation of target molecule(s) and their spatial correlation with the…”
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Journal Article -
4
Super-resolution fluorescence microscopy by line-scanning with an unmodified two-photon microscope
Published in Philosophical transactions of the Royal Society of London. Series A: Mathematical, physical, and engineering sciences (14-06-2021)“…Fluorescence-based microscopy as one of the standard tools in biomedical research benefits more and more from super-resolution methods, which offer enhanced…”
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Journal Article -
5
Open-source microscope add-on for structured illumination microscopy
Published in Nature communications (20-02-2024)“…Super-resolution techniques expand the abilities of researchers who have the knowledge and resources to either build or purchase a system. This excludes the…”
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Journal Article -
6
A closed 3D printed microfluidic device for automated growth and differentiation of cerebral organoids from single‐cell suspension
Published in Biotechnology journal (01-08-2024)“…The development of 3D organoids has provided a valuable tool for studying human tissue and organ development in vitro. Cerebral organoids, in particular, offer…”
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Journal Article -
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Geometric Control of Cell Behavior by Biomolecule Nanodistribution
Published in ACS biomaterials science & engineering (14-11-2022)“…Many dynamic interactions within the cell microenvironment modulate cell behavior and cell fate. However, the pathways and mechanisms behind cell–cell or…”
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Journal Article -
8
Imaging tissues and cells beyond the diffraction limit with structured illumination microscopy and Bayesian image reconstruction
Published in Gigascience (01-01-2019)“…Structured illumination microscopy (SIM) is a family of methods in optical fluorescence microscopy that can achieve both optical sectioning and…”
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Journal Article -
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Quantitative super-resolution single molecule microscopy dataset of YFP-tagged growth factor receptors
Published in Gigascience (01-03-2018)“…Super-resolution single molecule localization microscopy (SMLM) is a method for achieving resolution beyond the classical limit in optical microscopes (approx…”
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Journal Article -
10
Toward Structured Macroporous Hydrogel Composites: Electron Beam-Initiated Polymerization of Layered Cryogels
Published in Biomacromolecules (13-04-2015)“…The ability to tailor mechanical properties and architecture is crucial in creating macroporous hydrogel scaffolds for tissue engineering. In the present work,…”
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Journal Article -
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Differentiation of neural rosettes from human pluripotent stem cells in vitro is sequentially regulated on a molecular level and accomplished by the mechanism reminiscent of secondary neurulation
Published in Stem cell research (01-10-2019)“…Development of neural tube has been extensively modeled in vitro using human pluripotent stem cells (hPSCs) that are able to form radially organized cellular…”
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Journal Article -
12
Interpolation methods for the improvement of the point spread function estimation
Published in 2018 28th International Conference Radioelektronika (RADIOELEKTRONIKA) (01-04-2018)“…Interpolation methods face the issue of the specific content of the images. This paper is devoted to a comparison of interpolation methods suitable for small…”
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Conference Proceeding -
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Macroporous bioceramic scaffolds based on tricalcium phosphates reinforced with silica: microstructural, mechanical, and biological evaluation
Published in Journal of Asian Ceramic Societies (03-04-2022)“…The positive effect of silica on microstructural, mechanical and biological properties of calcium phosphate scaffolds was investigated in this study. Scaffolds…”
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Journal Article -
14
Kompaktní Mikroskopie se Strukturním Osvětlením a Hodnocení Výkonu v Superrezolučním Zobrazování ŽIvých Buněk
Published 01-01-2021“…Structured illumination microscopy (SIM) is one of the most common means of achieving a spatial resolution beyond the diffraction limit in fluorescence…”
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Dissertation -
15
A Comprehensive In Vitro Comparison of Preparation Techniques for Fat Grafting
Published in Plastic and reconstructive surgery (1963) (01-03-2017)“…BACKGROUND:Lipomodelling is a technique that uses patient’s own fat for tissue regeneration and augmentation. The extent of regenerative effect is reported to…”
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Journal Article -
16
Automatic detection of voice onset time in dysarthric speech
Published in 2015 IEEE International Conference on Acoustics, Speech and Signal Processing (ICASSP) (01-04-2015)“…Although a number of speech disorders reflect varying involvement of brain areas, recently published automatic speech analyses have primarily been limited to…”
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Conference Proceeding -
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Phase estimation of illumination pattern in structured illumination microscopy
Published in 2017 27th International Conference Radioelektronika (RADIOELEKTRONIKA) (01-04-2017)“…Conventional structured illumination microscopy (SIM) methods require precise knowledge of the illumination pattern and assume the sample to be stationary…”
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Conference Proceeding