Preparation of glutamine films on silicon substrates

Amino acids and polypeptides thin films, pure or attached to polymers, present large application as sensors and biosensors. The interactions between such films and the supports, their sensorial properties, as well as the development of techniques to produce thin regular films, are still challenges i...

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Bibliographic Details
Published in:	Surface and interface analysis Vol. 40; no. 5; pp. 899 - 905
Main Authors:	Garcia, Irene T. S., Porto, Fabiane G. da S., do Amaral, Queila D. F., Carreño, Neftalí L. V., Martins, Márcio M., Wallau, Martin
Format:	Journal Article
Language:	English
Published:	Chichester, UK John Wiley & Sons, Ltd 01-05-2008 Wiley
Subjects:	amino acid Analytical chemistry Chemistry Condensed matter: electronic structure, electrical, magnetic, and optical properties Condensed matter: structure, mechanical and thermal properties Cross-disciplinary physics: materials science; rheology Exact sciences and technology Infrared and raman spectra and scattering Materials science Methods of deposition of films and coatings; film growth and epitaxy Miscellaneous Optical properties and condensed-matter spectroscopy and other interactions of matter with particles and radiation Organic compounds, polymers organic films Physics Rutherford backscattering (RBS), and other methods of chemical analysis Structure and morphology; thickness surface treatment Surfaces and interfaces; thin films and whiskers (structure and nonelectronic properties) Thin film structure and morphology Scanning electron microscopy RBS surface treatment organic films amino acid Surface treatments Contact angle Infrared spectra Surface structure Thin films Aqueous solutions Preparation Fourier transform spectra Pretreatment Organic compounds Glutamine
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Summary:	Amino acids and polypeptides thin films, pure or attached to polymers, present large application as sensors and biosensors. The interactions between such films and the supports, their sensorial properties, as well as the development of techniques to produce thin regular films, are still challenges in the area. In this work, we present the preparation of L‐glutamine thin films on silicon substrates, the factors that determine amino acid/silicon substrate interaction, and the morphology of the films. For this purpose, a 24 factorial design is used, taking into account the effects of the solvent system, the glutamine concentration, the temperature, as well as the pretreatment of the substrate surface. The contact angles between a drop of glutamine solution and the silicon substrate were taken for the preliminary evaluation of the affinity between the amino acid and the substrate. The results have shown six promising experimental combinations with oxidized silicon as substrate to improve the solution/substrate interaction. Three of these promising conditions involved aqueous solution of L‐glutamine and three alkalis solution. The obtained films were characterized by scanning electron microscopy (SEM), Rutherford backscattering (RBS), and Fourier transform infrared (FTIR) spectroscopy. The selected experimental conditions permitted to prepare a variety of films with L‐glutamine, like small crystals, lamellas, needles, and smoothed regular films. The systems prepared in presence of alkalis solution yielded regular and smooth films. Copyright © 2008 John Wiley & Sons, Ltd.
Bibliography:	ark:/67375/WNG-1NJBKJ2R-G istex:35BC487669E29B326B1EA188C496442911C733B4 ArticleID:SIA2786 FAPERGS/CNPq - No. EDITAL 03/2004-PRONEX/CT&I (process number 05/11463) CNPq - No. /CGN PIBIC-process number 113038/2005-4 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0142-2421 1096-9918
DOI:	10.1002/sia.2786