Search Results - "Portaliou, Athina G."

Type III Secretion: Building and Operating a Remarkable Nanomachine by Portaliou, Athina G., Tsolis, Konstantinos C., Loos, Maria S., Zorzini, Valentina, Economou, Anastassios

“…The Type III secretion system (T3SS) is a protein export pathway that is widespread in Gram-negative bacteria and delivers effector proteins directly into…”
Get full text

Preproteins couple the intrinsic dynamics of SecA to its ATPase cycle to translocate via a catch and release mechanism by Krishnamurthy, Srinath, Sardis, Marios-Frantzeskos, Eleftheriadis, Nikolaos, Chatzi, Katerina E., Smit, Jochem H., Karathanou, Konstantina, Gouridis, Giorgos, Portaliou, Athina G., Bondar, Ana-Nicoleta, Karamanou, Spyridoula, Economou, Anastassios

“…Protein machines undergo conformational motions to interact with and manipulate polymeric substrates. The Sec translocase promiscuously recognizes, becomes…”
Get full text

Structural Instability Tuning as a Regulatory Mechanism in Protein-Protein Interactions by Chen, Li, Balabanidou, Vassilia, Remeta, David P., Minetti, Conceição A.S.A., Portaliou, Athina G., Economou, Anastassios, Kalodimos, Charalampos G.

“…Protein-protein interactions mediate a vast number of cellular processes. Here, we present a regulatory mechanism in protein-protein interactions mediated by…”
Get full text

Substrate-Activated Conformational Switch on Chaperones Encodes a Targeting Signal in Type III Secretion by Chen, Li, Ai, Xuanjun, Portaliou, Athina G., Minetti, Conceicao A.S.A., Remeta, David P., Economou, Anastassios, Kalodimos, Charalampos G.

“…The targeting of type III secretion (TTS) proteins at the injectisome is an important process in bacterial virulence. Nevertheless, how the injectisome…”
Get full text

A Reporter System for Fast Quantitative Monitoring of Type 3 Protein Secretion in Enteropathogenic E. coli by Barkalita, Luit, Portaliou, Athina G., Loos, Maria S., Yuan, Biao, Karamanou, Spyridoula, Economou, Anastassios

“…The type 3 secretion system is essential for pathogenesis of several human and animal Gram-negative bacterial pathogens. The T3SS comprises a transmembrane…”
Get full text

Trigger factor is a bona fide secretory pathway chaperone that interacts with SecB and the translocase by De Geyter, Jozefien, Portaliou, Athina G, Srinivasu, Bindu, Krishnamurthy, Srinath, Economou, Anastassios, Karamanou, Spyridoula

“…Bacterial secretory preproteins are translocated across the inner membrane post‐translationally by the SecYEG‐SecA translocase. Mature domain features and…”
Get full text

Evolutionary adaptation of the protein folding pathway for secretability by Smets, Dries, Tsirigotaki, Alexandra, Smit, Jochem H, Krishnamurthy, Srinath, Portaliou, Athina G, Vorobieva, Anastassia, Vranken, Wim, Karamanou, Spyridoula, Economou, Anastassios

“…Secretory preproteins of the Sec pathway are targeted post‐translationally and cross cellular membranes through translocases. During cytoplasmic transit,…”
Get full text

A nexus of intrinsic dynamics underlies translocase priming by Krishnamurthy, Srinath, Eleftheriadis, Nikolaos, Karathanou, Konstantina, Smit, Jochem H., Portaliou, Athina G., Chatzi, Katerina E., Karamanou, Spyridoula, Bondar, Ana-Nicoleta, Gouridis, Giorgos, Economou, Anastassios

“…The cytoplasmic ATPase SecA and the membrane-embedded SecYEG channel assemble to form the Sec translocase. How this interaction primes and catalytically…”
Get full text

The Preprotein Binding Domain of SecA Displays Intrinsic Rotational Dynamics by Vandenberk, Niels, Karamanou, Spyridoula, Portaliou, Athina G., Zorzini, Valentina, Hofkens, Johan, Hendrix, Jelle, Economou, Anastassios

“…SecA converts ATP energy to protein translocation work. Together with the membrane-embedded SecY channel it forms the bacterial protein translocase. How…”
Get full text

Substrate-Activated Conformational Switch on Chaperones Encodes a Targeting Signal in Type III Secretion by Chen, Li, Ai, Xuanjun, Portaliou, Athina G., Minetti, Conceicao A.S.A., Remeta, David P., Economou, Anastassios, Kalodimos, Charalampos G.

Get full text

Hierarchical protein targeting and secretion is controlled by an affinity switch in the type III secretion system of enteropathogenic Escherichia coli by Portaliou, Athina G, Tsolis, Konstantinos C, Loos, Maria S, Balabanidou, Vassileia, Rayo, Josep, Tsirigotaki, Alexandra, Crepin, Valerie F, Frankel, Gad, Kalodimos, Charalampos G, Karamanou, Spyridoula, Economou, Anastassios

“…Type III secretion (T3S), a protein export pathway common to Gram‐negative pathogens, comprises a trans‐envelope syringe, the injectisome, with a…”
Get full text

Chaperone Recycling in Late-Stage Flagellar Assembly by Rossi, Paolo, Xing, Qiong, Bini, Elisabetta, Portaliou, Athina G., Clay, Mary C., Warren, Eric M., Khanra, Nandish K., Economou, Anastassios, Kalodimos, Charalampos G.

“…[Display omitted] •Flagellum is assembled by Type III secretion system to obtain cell motility.•The NMR structures of the FliT-FliJ and FlgN-FliJ complexes are…”
Get full text

Structural Dynamics of the Functional Nonameric Type III Translocase Export Gate by Yuan, Biao, Portaliou, Athina G., Parakra, Rinky, Smit, Jochem H., Wald, Jiri, Li, Yichen, Srinivasu, Bindu, Loos, Maria S., Dhupar, Harveer Singh, Fahrenkamp, Dirk, Kalodimos, Charalampos G., Duong van Hoa, Franck, Cordes, Thorben, Karamanou, Spyridoula, Marlovits, Thomas C., Economou, Anastassios

“…[Display omitted] •Intrinsic dynamics of the type III protein translocase export gate SctV are unknown.•Self-nonamerized SctV ring is a high affinity…”
Get full text

Long-Lived Folding Intermediates Predominate the Targeting-Competent Secretome by Tsirigotaki, Alexandra, Chatzi, Katerina E., Koukaki, Marina, De Geyter, Jozefien, Portaliou, Athina G., Orfanoudaki, Georgia, Sardis, Marios Frantzeskos, Trelle, Morten Beck, Jørgensen, Thomas J.D., Karamanou, Spyridoula, Economou, Anastassios

“…Secretory preproteins carry signal peptides fused amino-terminally to mature domains. They are post-translationally targeted to cross the plasma membrane in…”
Get full text

Substrate-Activated Conformational Switch on Chaperones Encodes aTargeting Signal in Type III Secretion by Chen, Li, Ai, Xuanjun, Portaliou, Athina G., Minetti, Conceicao A.S.A., Remeta, David P., Economou, Anastassios, Kalodimos, Charalampos G.

“…Targeting of type III secretion proteins at the injectisome is an important process in bacterial virulence. Nevertheless, how the injectisome specifically…”
Get full text