Search Results - "Porrini, Massimiliano"

Dynamics of Intact Immunoglobulin G Explored by Drift-Tube Ion-Mobility Mass Spectrometry and Molecular Modeling by Pacholarz, Kamila J., Porrini, Massimiliano, Garlish, Rachel A., Burnley, Rebecca J., Taylor, Richard J., Henry, Alistair J., Barran, Perdita E.

“…Collision cross‐sections (CCS) of immunoglobulins G1 and G4 have been determined using linear drift‐tube ion‐mobility mass spectrometry. Intact antibodies and…”
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Compaction of Duplex Nucleic Acids upon Native Electrospray Mass Spectrometry by Porrini, Massimiliano, Rosu, Frédéric, Rabin, Clémence, Darré, Leonardo, Gómez, Hansel, Orozco, Modesto, Gabelica, Valérie

“…We report on the fate of nucleic acids conformation in the gas phase as sampled using native mass spectrometry coupled to ion mobility spectrometry. On the…”
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The effect of salt on the conformations of three model proteins is revealed by variable temperature ion mobility mass spectrometry by Berezovskaya, Yana, Porrini, Massimiliano, Barran, Perdita E.

“…[Display omitted] ► We examine the effect of sodium iodide on three model proteins using nESI-IM-MS. ► The number of iodides correlates to the number of…”
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Orienting Tetramolecular G-Quadruplex Formation: The Quest for the Elusive RNA Antiparallel Quadruplex by Mendoza, Oscar, Porrini, Massimiliano, Salgado, Gilmar F., Gabelica, Valérie, Mergny, Jean-Louis

“…DNA and RNA G‐quadruplexes (G4) are unusual nucleic acid structures involved in a number of key biological processes. RNA G‐quadruplexes are less studied…”
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The use of ion mobility mass spectrometry to assist protein design: a case study on zinc finger fold versus coiled coil interactions by Berezovskaya, Yana, Porrini, Massimiliano, Nortcliffe, Chris, Barran, Perdita E

“…The dramatic conformational change in zinc fingers on binding metal ions for DNA recognition makes their structure-function behaviour an attractive target to…”
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Metal binding to a zinc-finger peptide: a comparison between solution and the gas phase by Berezovskaya, Yana, Armstrong, Craig T, Boyle, Aimee L, Porrini, Massimiliano, Woolfson, Derek N, Barran, Perdita E

“…Solution-phase spectroscopy and mass spectrometry are used to probe interactions between divalent metal ions and a synthetic Cys(2)His(2) zinc-finger peptide…”
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Linking molecular models with ion mobility experiments. Illustration with a rigid nucleic acid structure by D'Atri, Valentina, Porrini, Massimiliano, Rosu, Frédéric, Gabelica, Valérie

“…Ion mobility spectrometry experiments allow the mass spectrometrist to determine an ion's rotationally averaged collision cross section ΩEXP. Molecular…”
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Electronic spectroscopy of isolated DNA polyanions by Daly, Steven, Porrini, Massimiliano, Rosu, Frédéric, Gabelica, Valérie

“…In solution, UV-vis spectroscopy is often used to investigate structural changes in biomolecules (e.g., nucleic acids), owing to changes in the environment of…”
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Compaction of RNA Hairpins and Their Kissing Complexes in Native Electrospray Mass Spectrometry by Abi-Ghanem, Josephine, Rabin, Clémence, Porrini, Massimiliano, Rosu, Frédéric, Gabelica, Valérie

“…When electrosprayed from typical native MS solution conditions, RNA hairpins and kissing complexes acquire charge states at which they get significantly more…”
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Electrostatics Explains the Position-Dependent Effect of GU Wobble Base Pairs on the Affinity of RNA Kissing Complexes by Abi-Ghanem, Josephine, Rabin, Clémence, Porrini, Massimiliano, Dausse, Eric, Toulme, Jean-Jacques, Gabelica, Valérie

“…In the RNA realm, non-Watson-Crick base pairs are abundant and can affect both the RNA 3D structure and its function. Here, we investigated the formation of…”
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Small-Molecule Inhibition of c‑MYC:MAX Leucine Zipper Formation Is Revealed by Ion Mobility Mass Spectrometry by Harvey, Sophie R, Porrini, Massimiliano, Stachl, Christiane, MacMillan, Derek, Zinzalla, Giovanna, Barran, Perdita E

“…The leucine zipper interaction between MAX and c-MYC has been studied using mass spectrometry and drift time ion mobility mass spectrometry (DT IM-MS) in…”
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Hybrid Mass Spectrometry Approaches to Determine How L-Histidine Feedback Regulates the Enzyzme MtATP-Phosphoribosyltransferase by Pacholarz, Kamila J., Burnley, Rebecca J., Jowitt, Thomas A., Ordsmith, Victoria, Pisco, João Pedro, Porrini, Massimiliano, Larrouy-Maumus, Gérald, Garlish, Rachel A., Taylor, Richard J., de Carvalho, Luiz Pedro Sório, Barran, Perdita E.

“…MtATP-phosphoribosyltransferase (MtATP-PRT) is an enzyme catalyzing the first step of the biosynthesis of L-histidine in Mycobacterium tuberculosis, and…”
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Dissecting the Dynamic Conformations of the Metamorphic Protein Lymphotactin by Harvey, Sophie R, Porrini, Massimiliano, Konijnenberg, Albert, Clarke, David J, Tyler, Robert C, Langridge-Smith, Patrick R. R, MacPhee, Cait E, Volkman, Brian F, Barran, Perdita E

“…A mass spectrometer provides an ideal laboratory to probe the structure and stability of isolated protein ions. Interrogation of each discrete…”
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Early stages of insulin fibrillogenesis examined with ion mobility mass spectrometry and molecular modelling by Cole, Harriet, Porrini, Massimiliano, Morris, Ryan, Smith, Tom, Kalapothakis, Jason, Weidt, Stefan, Mackay, C Logan, MacPhee, Cait E, Barran, Perdita E

“…A prevalent type of protein misfolding causes the formation of β-sheet-rich structures known as amyloid fibrils. Research into the mechanisms of fibril…”
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Effect of Protonation State on the Stability of Amyloid Oligomers Assembled from TTR(105–115) by Porrini, Massimiliano, Zachariae, Ulrich, Barran, Perdita E, MacPhee, Cait E

“…Amyloid fibrils are self-assembled aggregates of polypeptides that are implicated in the development of several human diseases. A peptide derived from amino…”
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Electron capture dissociation and drift tube ion mobility-mass spectrometry coupled with site directed mutations provide insights into the conformational diversity of a metamorphic protein by Harvey, Sophie R, Porrini, Massimiliano, Tyler, Robert C, MacPhee, Cait E, Volkman, Brian F, Barran, Perdita E

“…Ion mobility mass spectrometry can be combined with data from top-down sequencing to discern adopted conformations of proteins in the absence of solvent. This…”
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Linking molecular models with ion mobility experiments. Illustration with a rigid nucleic acid structure by D'Atri, Valentina, Porrini, Massimiliano, Rosu, Frédéric, Gabelica, Valérie

“…The preservation of molecular ion three dimensional conformations of electrospray formed ions is a question that many researchers have asked and that can now…”
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Frontispiece: Orienting Tetramolecular G-Quadruplex Formation: The Quest for the Elusive RNA Antiparallel Quadruplex by Mendoza, Oscar, Porrini, Massimiliano, Salgado, Gilmar F., Gabelica, Valérie, Mergny, Jean-Louis

“…G‐Quadruplexes Duplex DNA formation allows the predictable construction of nanomaterials with a large number of potential applications. Additionally, duplexes…”
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Electrostatics Explains the Position‐Dependent Effect of G⋅U Wobble Base Pairs on the Affinity of RNA Kissing Complexes by Abi‐Ghanem, Josephine, Rabin, Clémence, Porrini, Massimiliano, Dausse, Eric, Toulmé, Jean‐Jacques, Gabelica, Valérie

“…In the RNA realm, non‐Watson–Crick base pairs are abundant and can affect both the RNA 3D structure and its function. Here, we investigated the formation of…”
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Probing the Conformational Diversity of Cancer-Associated Mutations in p53 with Ion-Mobility Mass Spectrometry by Jurneczko, Ewa, Cruickshank, Faye, Porrini, Massimiliano, Clarke, David J., Campuzano, Iain D. G., Morris, Michael, Nikolova, Penka V., Barran, Perdita E.

“…Conformational flexibility: The DNA‐binding domain of tumor suppressor protein p53 (see picture) is characterized by using ion‐mobility mass spectrometry…”
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