MicroRNA and protein profiles in invasive versus non-invasive oral tongue squamous cell carcinoma cells in vitro

Complex molecular pathways regulate cancer invasion. This study overviewed proteins and microRNAs (miRNAs) involved in oral tongue squamous cell carcinoma (OTSCC) invasion. The human highly aggressive OTSCC cell line HSC-3 was examined in a 3D organotypic human leiomyoma model. Non-invasive and inva...

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Published in:	Experimental cell research Vol. 350; no. 1; pp. 9 - 18
Main Authors:	Korvala, Johanna, Jee, Kowan, Porkola, Emmi, Almangush, Alhadi, Mosakhani, Neda, Bitu, Carolina, Cervigne, Nilva K., Zandonadi, Flávia S., Meirelles, Gabriela V., Leme, Adriana Franco Paes, Coletta, Ricardo D., Leivo, Ilmo, Salo, Tuula
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 01-01-2017 Elsevier BV
Subjects:	60 APPLIED LIFE SCIENCES ADHESION Bioinformatics Carcinoma, Squamous Cell - genetics Carcinoma, Squamous Cell - metabolism Carcinoma, Squamous Cell - pathology CARCINOMAS CATTLE Cell adhesion & migration Cell Adhesion - genetics Cell Line, Tumor Cell Movement - genetics CELL PROLIFERATION Cell Proliferation - genetics Down-Regulation Gene Expression Regulation, Neoplastic Humans IN VITRO MASS SPECTROSCOPY Metastasis MicroRNA MicroRNAs MicroRNAs - genetics MIGRATION Neoplasm Invasiveness Oral cancer Oral tongue squamous cell carcinoma POLYMERASE CHAIN REACTION Protein expression Proteomics RECEPTORS Tongue Neoplasms - genetics Tongue Neoplasms - metabolism Tongue Neoplasms - pathology TRANSCRIPTION VIABILITY DMEM IIS HSC-3 MicroRNA FBS qRT-PCR Proteomics miRNA Oral tongue squamous cell carcinoma Bioinformatics ECM SCC-15
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Summary:	Complex molecular pathways regulate cancer invasion. This study overviewed proteins and microRNAs (miRNAs) involved in oral tongue squamous cell carcinoma (OTSCC) invasion. The human highly aggressive OTSCC cell line HSC-3 was examined in a 3D organotypic human leiomyoma model. Non-invasive and invasive cells were laser-captured and protein expression was analyzed using mass spectrometry-based proteomics and miRNA expression by microarray. In functional studies the 3D invasion assay was replicated after silencing candidate miRNAs, miR-498 and miR-940, in invasive OTSCC cell lines (HSC-3 and SCC-15). Cell migration, proliferation and viability were also studied in the silenced cells. In HSC-3 cells, 67 proteins and 53 miRNAs showed significant fold-changes between non-invasive vs. invasive cells. Pathway enrichment analyses allocated “Focal adhesion” and “ECM-receptor interaction” as most important for invasion. Significantly, in HSC-3 cells, miR-498 silencing decreased the invasion area and miR-940 silencing reduced invasion area and depth. Viability, proliferation and migration weren’t significantly affected. In SCC-15 cells, down-regulation of miR-498 significantly reduced invasion and migration. This study shows HSC-3 specific miRNA and protein expression in invasion, and suggests that miR-498 and miR-940 affect invasion in vitro, the process being more influenced by mir-940 silencing in aggressive HSC-3 cells than in the less invasive SCC-15.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0014-4827 1090-2422
DOI:	10.1016/j.yexcr.2016.10.015