Search Results - "Popot, J"

Thermodynamic Characterization of the Exchange of Detergents and Amphipols at the Surfaces of Integral Membrane Proteins by Tribet, C, Diab, C, Dahmane, T, Zoonens, M, Popot, J.-L, Winnik, F. M

“…The aggregation of integral membrane proteins (IMPs) in aqueous media is a significant concern for mechanistic investigations and pharmaceutical applications…”
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High-Resolution Structure of a Membrane Protein Transferred from Amphipol to a Lipidic Mesophase by Polovinkin, V., Gushchin, I., Sintsov, M., Round, E., Balandin, T., Chervakov, P., Schevchenko, V., Utrobin, P., Popov, A., Borshchevskiy, V., Mishin, A., Kuklin, A., Willbold, D., Chupin, V., Popot, J.-L., Gordeliy, V.

“…Amphipols (APols) have become important tools for the stabilization, folding, and in vitro structural and functional studies of membrane proteins (MPs). Direct…”
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In Vivo Characterization of the Biodistribution Profile of Amphipol A8–35 by Fernandez, A., Le Bon, C., Baumlin, N., Giusti, F., Crémel, G., Popot, J.-L., Bagnard, D.

“…Amphipols (APols) are polymeric surfactants that keep membrane proteins (MPs) water-soluble in the absence of detergent, while stabilizing them. They can be…”
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HELICAL MEMBRANE PROTEIN FOLDING, STABILITY, AND EVOLUTION by Popot, Jean-Luc, Engelman, Donald M

“…Helical membrane protein folding and oligomerization can be usefully conceptualized as involving two energetically distinct stages-the formation and subsequent…”
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Complexation of integral membrane proteins by phosphorylcholine-based amphipols by Diab, C., Tribet, C., Gohon, Y., Popot, J.-L., Winnik, F.M.

“…Amphiphilic macromolecules, known as amphipols, have emerged as promising candidates to replace conventional detergents for handling integral membrane proteins…”
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Nanoparticle Surface-Enhanced Raman Scattering of Bacteriorhodopsin Stabilized by Amphipol A8-35 by Polovinkin, V., Balandin, T., Volkov, O., Round, E., Borshchevskiy, V., Utrobin, P., von Stetten, D., Royant, A., Willbold, D., Arzumanyan, G., Chupin, V., Popot, J.-L., Gordeliy, V.

“…Surface-enhanced Raman spectroscopy (SERS) has developed dramatically since its discovery in the 1970s, because of its power as an analytical tool for…”
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Amphipols: polymeric surfactants for membrane biology research by Popot, J-L, Berry, E A, Charvolin, D, Creuzenet, C, Ebel, C, Engelman, D M, Flötenmeyer, M, Giusti, F, Gohon, Y, Hong, Q, Lakey, J H, Leonard, K, Shuman, H A, Timmins, P, Warschawski, D E, Zito, F, Zoonens, M, Pucci, B, Tribet, C

“…Membrane proteins classically are handled in aqueous solutions as complexes with detergents. The dissociating character of detergents, combined with the need…”
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Membrane protein folding and oligomerization: the two-stage model by Popot, J. L, Engelman, D. M

“…We discuss the view that the folding of many, perhaps most, integral membrane proteins can be considered as a two-stage process. In stage I, hydrophobic…”
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Amphipols: Polymers that Keep Membrane Proteins Soluble in Aqueous Solutions by Tribet, Christophe, Audebert, Roland, Popot, Jean-Luc

“…Amphipols are a new class of surfactants that make it possible to handle membrane proteins in detergent-free aqueous solution as though they were soluble…”
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Erratum to: High-Resolution Structure of a Membrane Protein Transferred from Amphipol to a Lipidic Mesophase by Polovinkin, V., Gushchin, I., Sintsov, M., Round, E., Balandin, T., Chervakov, P., Shevchenko, V., Utrobin, P., Popov, A., Borshchevskiy, V., Mishin, A., Kuklin, A., Willbold, D., Chupin, V., Popot, J.-L., Gordeliy, V.

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Mutations in a new gene encoding a protein of the hair bundle cause non-syndromic deafness at the DFNB16 locus by Lainé, Sophie, Masmoudi, Saber, Hutchin, Tim P, Verpy, Elisabeth, Leibovici, Michel, Nouaille, Sylvie, Del Castillo, Ignacio, Blanchard, Stéphane, Petit, Christine, Popot, Jean-Luc, Moreno, Felipe, Mueller, Robert F, Zwaenepoel, Ingrid

“…Hearing impairment affects about 1 in 1,000 children at birth. Approximately 70 loci implicated in non-syndromic forms of deafness have been reported in humans…”
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Stabilization of Hydrophobic Colloidal Dispersions in Water with Amphiphilic Polymers:  Application to Integral Membrane Proteins by Tribet, C, Audebert, R, Popot, J.-L

“…Stabilization of aqueous dispersions of highly hydrophobic colloidal particleshere three integral membrane proteins of nanometric dimensionswere obtained by…”
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Nonionic amphiphilic polymers derived from Tris(hydroxymethyl)-acrylamidomethane keep membrane proteins soluble and native in the absence of detergent by Prata, C., Giusti, F., Gohon, Y., Pucci, B., Popot, J.-L., Tribet, C.

“…A new family of amphipols—amphiphilic polymers designed to form water‐soluble complexes with membrane proteins—was synthesized by free‐radical telomerization…”
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NMR Study of a Membrane Protein in Detergent-Free Aqueous Solution by Zoonens, Manuela, Catoire, Laurent J., Giusti, Fabrice, Popot, Jean-Luc, Engelman, Donald M.

“…One of the major obstacles to membrane protein (MP) structural studies is the destabilizing effect of detergents. Amphipols (APols) are short amphipathic…”
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Stabilization of integral membrane proteins in aqueous solution using fluorinated surfactants by Chabaud, E., Barthélémy, P., Mora, N., Popot, J.L., Pucci, B.

“…Surfactants carrying either a hydrocarbon or a fluorocarbon alkyl chain have been synthesized. The polar head was either tris(hydroxymethyl)acrylamidomethane…”
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Nicotinic receptor of acetylcholine: structure of an oligomeric integral membrane protein by Popot, J L, Changeux, J P

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chloroplast ycf7 (petL) open reading frame of Chlamydomonas reinhardtii encodes a small functionally important subunit of the cytochrome b6f complex by Takahashi, Y, Rahire, M, Breyton, C, Popot, J.L, Joliot, P, Rochaix, J.D

“…The small chloroplast open reading frame ORF43 (ycf7) of the green unicellular alga Chlamydomonas reinhardtii is cotranscribed with the psaC gene and ORF58…”
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Refolding of bacteriorhodopsin in lipid bilayers. A thermodynamically controlled two-stage process by Popot, J L, Gerchman, S E, Engelman, D M

“…Possible steps in the folding of bacteriorhodopsin are revealed by studying the refolding and interaction of two fragments of the molecule reconstituted in…”
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Interaction of Amphipols with Sarcoplasmic Reticulum Ca2+-ATPase by Champeil, Philippe, Menguy, Thierry, Tribet, Christophe, Popot, Jean-Luc, le Maire, Marc

“…Amphipols are short-chain amphipathic polymers designed to keep membrane proteins soluble in aqueous solutions. We have evaluated the effects of the…”
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Major myelin proteolipid the 4-α-helix topology by POPOT, J.-L, PHAM DINH, D, DAUTIGNY, A

“…Several conflicting models have been proposed for the membrane arrangement of the major myelin proteolipid (PLP). We have compared features of the sequence of…”
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