Pharmacological inhibition of store-operated calcium entry in MDA-MB-468 basal A breast cancer cells: consequences on calcium signalling, cell migration and proliferation
Store-operated Ca 2+ entry is a pathway that is remodelled in a variety of cancers, and altered expression of the components of store-operated Ca 2+ entry is a feature of breast cancer cells of the basal molecular subtype. Studies of store-operated Ca 2+ entry in breast cancer cells have used non-sp...
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| Published in: | Cellular and molecular life sciences : CMLS Vol. 75; no. 24; pp. 4525 - 4537 |
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| Main Authors: | , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Cham
Springer International Publishing
01-12-2018
Springer Nature B.V |
| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Store-operated Ca
2+
entry is a pathway that is remodelled in a variety of cancers, and altered expression of the components of store-operated Ca
2+
entry is a feature of breast cancer cells of the basal molecular subtype. Studies of store-operated Ca
2+
entry in breast cancer cells have used non-specific pharmacological inhibitors, complete depletion of intracellular Ca
2+
stores and have mostly focused on MDA-MB-231 cells (a basal B breast cancer cell line). These studies compared the effects of the selective store-operated Ca
2+
entry inhibitors Synta66 and YM58483 (also known as BTP2) on global cytosolic free Ca
2+
([Ca
2+
]
CYT
) changes induced by physiological stimuli in a different breast cancer basal cell line model, MDA-MB-468. The effects of these agents on proliferation as well as serum and epidermal growth factor (EGF) induced migration were also assessed. Activation with the purinergic receptor activator adenosine triphosphate, produced a sustained increase in [Ca
2+
]
CYT
that was entirely dependent on store-operated Ca
2+
entry. The protease activated receptor 2 activator, trypsin, and EGF also produced Ca
2+
influx that was sensitive to both Synta66 and YM58483. Serum-activated migration of MDA-MB-468 breast cancer cells was sensitive to both store-operated Ca
2+
inhibitors. However, proliferation and EGF-activated migration was differentially affected by Synta66 and YM58483. These studies highlight the need to define the exact mechanisms of action of different store-operated calcium entry inhibitors and the impact of such differences in the control of tumour progression pathways. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 1420-682X 1420-9071 |
| DOI: | 10.1007/s00018-018-2904-y |