Synthesis and Functionalization of PLA-Knitted Fabrics with Silk Fibroin Nanoparticles and Chitosan: Evaluation of Cytotoxicity and Cell Adhesion

Synthesis and Functionalization of PLA-Knitted Fabrics with Silk Fibroin Nanoparticles and Chitosan: Evaluation of Cytotoxicity and Cell Adhesion

The substance principal used to synthesize these materials is fibroin, obtained from the silkworm’s cocoon ( Bombyx mori ) in the form of a filament, but can be converted into nanometric particles. After the synthesis, the obtained samples were characterized by size and Zeta potential (via nanosizer...

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Bibliographic Details
Published in:Fibers and polymers Vol. 25; no. 10; pp. 3751 - 3764
Main Authors: Pontes, Rayana Priscilla Silva, Ferreira, Myllena Kely Pereira, Borba, Pedro Brito, de Sá, Andrea Lima, de Andrade Neto, Valter Ferreira, dos Santos, Thiago Félix, Almeida, Raphael Lucas Jacinto, do Nascimento, José Heriberto Oliveira
Format: Journal Article
Language:English
Published: Seoul The Korean Fiber Society 01-10-2024
Springer Nature B.V
한국섬유공학회
Subjects:
Biocompatibility
Biomedical materials
Cell adhesion
Cell adhesion & migration
Chemistry
Chemistry and Materials Science
Chitosan
Cytotoxicity
Fourier transforms
Infrared analysis
Infrared spectroscopy
Low concentrations
Nanoparticles
Polylactic acid
Polymer Sciences
Regular Article
Silk fibroin
Silkworms
Synthesis
Ternary systems
Toxicity testing
X-ray diffraction
Zeta potential
섬유공학
Medical textiles
Cell viability
Biomaterial
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Summary:The substance principal used to synthesize these materials is fibroin, obtained from the silkworm’s cocoon ( Bombyx mori ) in the form of a filament, but can be converted into nanometric particles. After the synthesis, the obtained samples were characterized by size and Zeta potential (via nanosizer), X-Ray diffraction (XRD), specific area determination by the BET method, Infrared Spectroscopy with Fourier Transform with attenuated diffuse reflectance (FTIR), transmission electron microscopy (TEM). The silk fibroin nanoparticles (SFNp) were immobilized on polylactic acid (PLA)-knitted fabric and functionalized with chitosan for FTIR and XRD analyses, in addition to cytotoxicity analysis with colorimetric test (MTT) and cell adhesion. The DLS, FTIR and MET results confirm the formation of spherical silk fibroin nanoparticles with average sizes between 48 and 156nm, and zeta potential of − 19.95mV which characterizes a good dispersibility. Cell-viability assessment showed that SFNp is a non-cytotoxic material at low concentrations up to 48h, confirming that the nanoparticle synthesis method using the ternary system is an effective and low-cost alternative. This technique has great potential for use in the development of biomaterials, due to the production of knitted tissues with PLA functionalized with chitosan and immobilized with SFNp, exhibiting mechanical strength and biocompatibility.
ISSN:1229-9197
1875-0052
DOI:10.1007/s12221-024-00690-6