Fluorescent quantum dot-based nanotool for targeted identification and evaluation of the schistosomiasis circulating cathodic antigen in tissue samples

Schistosomiasis represents a serious public health problem, a disease for which the circulating cathodic antigen (CCA) is a relevant biomarker. Quantum dots (QDs) are advantageous fluorescent nanoparticles that can be used as specific nanoprobes. In this study, a nanotool based on QDs and anti-CCA a...

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Published in:	Micron (Oxford, England : 1993) Vol. 183; p. 103658
Main Authors:	Lima, Rennan R.M., Pontes, Maria S.S., Silva, Mychel G., Ribeiro, Jéssika F.F., Nascimento, Gabriela A.F., Grenfell, Rafaella F.Q., Melo-Jr, Mário R., de Paula, Ana M., Pereira, Goreti, Cabral Filho, Paulo E., Carvalho, Luiz B., Fontes, Adriana
Format:	Journal Article
Language:	English
Published:	England Elsevier Ltd 01-08-2024
Subjects:	Animals Anti-CCA antibody Antigens, Helminth - analysis Antigens, Helminth - immunology Female Fluorescence microscopy Kidney Kidney - parasitology Liver Liver - parasitology Mice Microscopy, Fluorescence - methods Nanoparticle Quantum Dots Schistosomiasis - diagnosis Schistosomiasis - parasitology Anti-CCA antibody Mice Nanoparticle Liver Kidney Fluorescence microscopy
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Summary:	Schistosomiasis represents a serious public health problem, a disease for which the circulating cathodic antigen (CCA) is a relevant biomarker. Quantum dots (QDs) are advantageous fluorescent nanoparticles that can be used as specific nanoprobes. In this study, a nanotool based on QDs and anti-CCA antibodies was developed, which, in association with fluorescence microscopy, was applied to trace and evaluate the CCA profile in schistosomiasis-infected tissue samples. Kidney and liver tissues from mice at different disease phases were used as models. QDs and the conjugates were characterized by absorption and emission spectroscopies. Microscopy analyses were used to map and assess CCA accumulation in infected tissue slices in respect to non-infected control samples. The fluorescent microplate assay (FMA) and Zeta potential (ζ) analyses indicated an effective conjugation, which was corroborated by the absence of labeling in non-infected tissue slices (which lack CCA) after incubation with the nanoprobe. Infected liver and kidney tissues exhibited notable staining by the QDs-anti-CCA conjugate. The CCA accumulation increased as follows: 30 < 60 = 120 days post-infection, with 30, 60, and 120 days corresponding to the pre-patent, acute, and beginning of chronic disease phases, respectively. Therefore, this innovative approach, combining imaging acquisition with the sensitivity and specificity of the QDs-anti-CCA conjugate, demonstrated efficiency in locating and comparatively evaluating CCA deposition in biological samples, thereby opening new possibilities for schistosomiasis research. [Display omitted] •Circulating cathodic antigen (CCA) is an important biomarker of schistosomiasis.•A fluorescent QDs-anti-CCA nanotool was developed.•The conjugate was applied to trace and evaluate the CCA in infected mice tissue samples.•Infected kidney and liver tissues were remarkably stained by the conjugate, from the 60th day of infection.•The nanotool combined with microscopy showed potential to identify the CCA, collaborating to schistosomiasis studies.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0968-4328 1878-4291 1878-4291
DOI:	10.1016/j.micron.2024.103658