On the Regularities of the Polar Profiles of Proteins Related to Ebola Virus Infection and their Functional Domains

The number of fatalities and economic losses caused by the Ebola virus infection across the planet culminated in the havoc that occurred between August and November 2014. However, little is known about the molecular protein profile of this devastating virus. This work represents a thorough bioinform...

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Bibliographic Details
Published in:	Cell biochemistry and biophysics Vol. 76; no. 3; pp. 411 - 431
Main Authors:	Polanco, Carlos, Samaniego Mendoza, José Lino, Buhse, Thomas, Uversky, Vladimir N., Bañuelos Chao, Ingrid Paola, Bañuelos Cedano, Marcela Angola, Tavera, Fernando Michel, Tavera, Daniel Michel, Falconi, Manuel, Ponce de León, Abelardo Vela
Format:	Journal Article
Language:	English
Published:	New York Springer US 01-09-2018 Springer Nature B.V
Subjects:	Amino acids Biochemistry Bioinformatics Biological and Medical Physics Biomedical and Life Sciences Biophysics Biotechnology Cell Biology Charge distribution Computational Biology - methods Databases, Protein Ebola virus Ebolavirus Ebolavirus - metabolism Economic impact Epidemics Hemorrhagic Fever, Ebola - metabolism Hemorrhagic Fever, Ebola - pathology Hemorrhagic Fever, Ebola - virology Humans Intrinsically Disordered Proteins - chemistry Intrinsically Disordered Proteins - metabolism Life Sciences Membrane Transport Proteins - chemistry Membrane Transport Proteins - metabolism Models, Theoretical Original Paper Pharmacology/Toxicology Proteins Viral diseases Viral Proteins - chemistry Viral Proteins - metabolism Proteins Proteomics Functional domains Structural Bioinformatics Synthetic proteins Human proteins interacting with Ebola virus Ebola virus proteins Ebola virus disease
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Summary:	The number of fatalities and economic losses caused by the Ebola virus infection across the planet culminated in the havoc that occurred between August and November 2014. However, little is known about the molecular protein profile of this devastating virus. This work represents a thorough bioinformatics analysis of the regularities of charge distribution (polar profiles) in two groups of proteins and their functional domains associated with Ebola virus disease: Ebola virus proteins and Human proteins interacting with Ebola virus. Our analysis reveals that a fragment exists in each of these proteins—one named the “functional domain”—with the polar profile similar to the polar profile of the protein that contains it. Each protein is formed by a group of short sub-sequences, where each fragment has a different and distinctive polar profile and where the polar profile between adjacent short sub-sequences changes orderly and gradually to coincide with the polar profile of the whole protein. When using the charge distribution as a metric, it was observed that it effectively discriminates the proteins from their functional domains. As a counterexample, the same test was applied to a set of synthetic proteins built for that purpose, revealing that any of the regularities reported here for the Ebola virus proteins and human proteins interacting with Ebola virus were not present in the synthetic proteins. Our results indicate that the polar profile of each protein studied and its corresponding functional domain are similar. Thus, when building each protein from its functional domai—adding one amino acid at a time and plotting each time its polar profile—it was observed that the resulting graphs can be divided into groups with similar polar profiles.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1085-9195 1559-0283
DOI:	10.1007/s12013-018-0839-4