Search Results - "Pometun, Anastasia A."
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Genetic fusion of P450 BM3 and formate dehydrogenase towards self-sufficient biocatalysts with enhanced activity
Published in Scientific reports (04-11-2021)“…Fusion of multiple enzymes to multifunctional constructs has been recognized as a viable strategy to improve enzymatic properties at various levels such as…”
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Structure-Functional Examination of Novel Ribonucleoside Hydrolase C (RihC) from Limosilactobacillus reuteri LR1
Published in International journal of molecular sciences (30-12-2023)“…Ribonucleoside hydrolase C (RihC, EC 3.2.2.1, 3.2.2.2, 3.2.2.3, 3.2.2.7, 3.2.2.8) belongs to the family of ribonucleoside hydrolases Rih and catalyzes the…”
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Ribonucleoside Hydrolases–Structure, Functions, Physiological Role and Practical Uses
Published in Biomolecules (Basel, Switzerland) (01-09-2023)“…Ribonucleoside hydrolases are enzymes that catalyze the cleavage of ribonucleosides to nitrogenous bases and ribose. These enzymes are found in many organisms:…”
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Additivities for Soluble Recombinant Protein Expression in Cytoplasm of Escherichia coli
Published in Fermentation (Basel) (01-03-2024)“…Recombinant protein expression in Escherichia coli is a fundamental technique in molecular biology and biotechnology. This review provides a comprehensive…”
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Activation/Inactivation Role of Ionic Liquids on Formate Dehydrogenase from Pseudomonas sp. 101 and Its Mutated Thermostable Form
Published in ChemCatChem (13-08-2018)“…Ionic liquids (ILs) are used in numerous research areas including biocatalysis. The effect of ILs/water mixture on the activity of wild type and a more…”
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Efficient Assay and Marker Significance of NAD + in Human Blood
Published in Frontiers in medicine (19-05-2022)“…Oxidized nicotinamide adenine dinucleotide (NAD ) is a biological molecule of systemic importance. Essential role of NAD in cellular metabolism relies on the…”
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Lactobacilli and Klebsiella: Two Opposites in the Fight for Human Health
Published in Biochemistry (Moscow) (2024)“…The problem of antibiotic resistance is currently very acute. Numerous research and development of new antibacterial drugs are being carried out that could…”
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Chimeric versus isolated proteins: Biochemical characterization of the NADP+-dependent formate dehydrogenase from Pseudomonas sp. 101 fused with the Baeyer-Villiger monooxygenase from Thermobifida fusca
Published in International journal of biological macromolecules (31-12-2023)“…The expression of multifunctional proteins can facilitate the setup of a biotechnology process that requires multiple functions absolved by different proteins…”
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9
Speeding up SDS–PAGE: Theory and experiment
Published in Electrophoresis (01-08-2023)“…In order to accelerate Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE), here we propose an optimized version of the technique enabled by…”
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Advantages of formate dehydrogenase reaction for efficient NAD+ quantification in biological samples
Published in Analytical biochemistry (15-08-2020)“…The medical significance of NAD+-dependent metabolic regulation acquires increasing attention, demanding rapid and clinically feasible quantification of NAD+…”
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Study of the structure-function relationship of formate dehydrogenase- an important enzyme for Staphylococcus aureus biofilms by rational design
Published in Biochimie (01-01-2024)“…NAD -dependent formate dehydrogenase (FDH, EC 1.2.1.2) from the bacterium Staphylococcus aureus (SauFDH) plays an important role in the vital activity of this…”
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NAD+-Dependent Formate Dehydrogenase from Themotolerant Yeast Ogataea parapolymorpha: Properties and Protein Engineering of the N-Terminal Sequence
Published in Biochemistry (Moscow) (01-09-2023)“…Previously, the gene of formate dehydrogenase (FDH, EC 1.2.1.2) from the thermotolerant methylotrophic yeast Ogataea parapolymorpha DL 1 (OpaFDH) was cloned in…”
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NAD.sup.+-Dependent Formate Dehydrogenase from Themotolerant Yeast Ogataea parapolymorpha: Properties and Protein Engineering of the N-Terminal Sequence
Published in Biochemistry (Moscow) (01-09-2023)“…Previously, the gene of formate dehydrogenase (FDH, EC 1.2.1.2) from the thermotolerant methylotrophic yeast Ogataea parapolymorpha DL 1 (OpaFDH) was cloned in…”
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14
Structure–Functional Examination of Novel Ribonucleoside Hydrolase C from ILimosilactobacillus reuteri/I LR1
Published in International journal of molecular sciences (01-12-2023)“…Ribonucleoside hydrolase C (RihC, EC 3.2.2.1, 3.2.2.2, 3.2.2.3, 3.2.2.7, 3.2.2.8) belongs to the family of ribonucleoside hydrolases Rih and catalyzes the…”
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15
Additivities for Soluble Recombinant Protein Expression in Cytoplasm of IEscherichia coli/I
Published in Fermentation (Basel) (01-02-2024)“…Recombinant protein expression in Escherichia coli is a fundamental technique in molecular biology and biotechnology. This review provides a comprehensive…”
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