Search Results - "Pollard, T.D."

Characterization of two classes of small molecule inhibitors of Arp2/3 complex by Pollard, T. D, Nolen, B. J, Tomasevic, N, Russell, A, Pierce, D. W, Jia, Z, McCormick, C. D, Hartman, J, Sakowicz, R

“…Polymerization of actin filaments directed by the actin-related protein (Arp)2/3 complex supports many types of cellular movements. However, questions remain…”
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Affinity of alpha-actinin for actin determines the structure and mechanical properties of actin filament gels by Wachsstock, D.H., Schwartz, W.H., Pollard, T.D.

“…Proteins that cross-link actin filaments can either form bundles of parallel filaments or isotropic networks of individual filaments. We have found that…”
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Nucleotide exchange, structure, and mechanical properties of filaments assembled from ATP-actin and ADP-actin by POLLARD, T. D, GOLDBERG, I, SCHWARZ, W. H

“…Actin monomers with bound ATP, ADP, or fluorescent analogues of these nucleotides exchange the nucleotide on a second time scale, whereas filaments assembled…”
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Direct visualization by electron microscopy of the weakly bound intermediates in the actomyosin adenosine triphosphatase cycle by Pollard, T.D., Bhandari, D., Maupin, P., Wachsstock, D., Weeds, A.G., Zot, H.G.

“…We used a novel stopped-flow/rapid-freezing machine to prepare the transient intermediates in the actin-myosin adenosine triphosphatase (ATPase) cycle for…”
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Purification, characterization and crystallization of Acanthamoeba profilin expressed in Escherichia coli by Almo, S C, Pollard, T D, Way, M, Lattman, E E

“…Profilin (isoform I) from Acanthamoeba castellani was expressed in Escherichia coli using a bacteriophage T7-based expression vector. The recombinant material…”
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Hydrolysis of ATP by polymerizes actin depends on the bound divalent cation but not profilin by Blanchoin, L., Pollard, T.D.

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Actin by Pollard, T D

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Assembly of Acanthamoeba Myosin-II Minifilaments. Model of Anti-parallel Dimers Based on EM and X-ray Diffraction of 2D and 3D Crystals by Turbedsky, Kirsi, Pollard, Thomas D., Yeager, Mark

“…Current models suggest that the first step in the assembly of Acanthamoeba myosin-II is anti-parallel dimerization of the coiled-coil tails with an overlap of…”
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Analyzing the Interaction of ADF/Cofilin with Actin through Molecular Dynamics Simulations by Durresi, E., Dalhaimer, P., Pollard, T.D.

“…Actin is a cytoskeletal protein found in all eukaryotic cells. It is involved in cellular motility and cytokinesis and is essential for processes such as…”
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Cross-linker dynamics determine the mechanical properties of actin gels by Wachsstock, D.H., Schwarz, W.H., Pollard, T.D.

“…To evaluate the contributions of cross-linker dynamics and polymer deformation to the frequency-dependent stiffness of actin filament gels, we compared the…”
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Purification, characterization and crystallization of human platelet profilin expressed in Escherichia coli by Fedorov, A A, Pollard, T D, Almo, S C

“…Human platelet profilin was expressed in Escherichia coli using a T7 based expression vector. The recombinant material is similar to authentic human platelet…”
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Transient state kinetics tutorial using the kinetics simulation program, KINSIM by Wachsstock, D.H., Pollard, T.D.

“…This article provides an introduction to a computer tutorial on transient state kinetics. The tutorial uses our Macintosh version of the computer program,…”
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Cell biology: actin-binding protein evolution by Pollard, T.D

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Purification of a calcium-sensitive actin gelation protein from Acanthamoeba by Pollard, T D

“…A new calcium-sensitive actin filament cross-linking protein has been purified from Acanthamoeba. By gel electrophoresis in sodium dodecyl sulfate, the…”
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Characterization of a second myosin from Acanthamoeba castellanii by Pollard, T D, Stafford, W F, Porter, M E

“…We purified a 400,000 molecular weight myosin, myosin-II, from Acanthamoeba castellanii. The sequence of ion exchange chromatography, actomyosin precipitation,…”
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Ultrastructural characterization of F-actin isolated from Acanthamoeba castellanii and identification of cytoplasmic filaments as F-actin by reaction with rabbit heavy meromyosin by Pollard, T D, Shelton, E, Weihing, R R, Korn, E D

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Inhibition of Mg ++ ATPase activity of actin-activated Acanthamoeba myosin by muscle troponin-tropomyosin: implications for the mechanism of control of amoeba motility and muscle contraction by Pollard, T D, Eisenberg, E, Korn, E D, Kielley, W W

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