Search Results - "Polisky, B"

Interference-based detection of nucleic acid targets on optically coated silicon by Polisky, Barry, Jenison, Robert, Yang, Shao, Haeberli, Ayla

“…Sequence-specific detection of polynucleotides typically requires modified reporter probes that are labeled with radioactive, fluorescent, or luminescent…”
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A SELEX primer by Fitzwater, T, Polisky, B

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Silicon-based Biosensors for Rapid Detection of Protein or Nucleic Acid Targets by Jenison, Robert, La, Helen, Haeberli, Ayla, Ostroff, Rachel, Polisky, Barry

“…We developed a silicon-based biosensor that generates visual, qualitative results or quantitative results for the detection of protein or nucleic acid targets…”
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Potent and persistent in vivo anti-HBV activity of chemically modified siRNAs by Morrissey, David V, Lockridge, Jennifer A, Shaw, Lucinda, Blanchard, Karin, Jensen, Kristi, Breen, Wendy, Hartsough, Kimberly, Machemer, Lynn, Radka, Susan, Jadhav, Vasant, Vaish, Narendra, Zinnen, Shawn, Vargeese, Chandra, Bowman, Keith, Shaffer, Chris S, Jeffs, Lloyd B, Judge, Adam, MacLachlan, Ian, Polisky, Barry

“…The efficacy of lipid-encapsulated, chemically modified short interfering RNA (siRNA) targeted to hepatitis B virus (HBV) was examined in an in vivo mouse…”
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Autonomous replication and addition of telomerelike sequences to DNA microinjected into Paramecium tetraurelia macronuclei by GILLEY, D, PREER, J. R, AUFDERHEIDE, K. J, POLISKY, B

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The molecular organization of the Antennapedia locus of Drosophila by Scott, M P, Weiner, A J, Hazelrigg, T I, Polisky, B A, Pirrotta, V, Scalenghe, F, Kaufman, T C

“…The Antennapedia complex (ANT-C) of Drosophila is a cluster of genes involved in the regulation of morphogenesis, including at least three homoeotic loci…”
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Control of ColE1 DNA Replication: The rop Gene Product Negatively Affects Transcription from the Replication Primer Promoter by Cesareni, Gianni, Muesing, Mark A., Polisky, Barry

“…A 600-base-pair region essential for ColE1 and pMB1 plasmid replication contains two promoters responsible for the synthesis of two RNA molecules central to…”
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Analysis of establishment phase replication of the plasmid ColE1 by Merlin, S, Polisky, B

“…The replication regulatory mechanisms by which the small, multicopy plasmid ColE1 maintains a constant steady-state copy number have been extensively…”
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Diversity of Oligonucleotide Functions by Gold, L, Polisky, B, Uhlenbeck, O, Yarus, M

“…SELEX is a technology for the identification of high affinity oligonucleotide ligands. Large libraries of random sequence single-stranded oligonucleotides,…”
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Identification of DNA Segments Capable of Rescuing a Non-Mendelian Mutant in Paramecium by Kim, C. S, Preer-Jr, J. R, Polisky, B

“…The non-Mendelian mutant d48 of Paramecium tetraurelia contains micronuclear wild type A genes, but at autogamy and conjugation proper processing fails and new…”
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Characterization of the ColE1 Primer-RNA1 Complex: Analysis of a Domain of ColE1 RNA1 Necessary for Its Interaction with Primer RNA by Tamm, Joseph, Polisky, Barry

“…RNA1 is a small, plasmid-encoded transcript involved in the replication control of the plasmid ColE1. RNA1 blocks replication by preventing processing of the…”
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Transformation of Paramecium by Microinjection of a Cloned Serotype Gene by Godiska, Ronald, Aufderheide, Karl J., Gilley, David, Hendrie, Paul, Fitzwater, Tim, Preer, Louise B., Polisky, Barry, Preer, John R.

“…Paramecia of a given serotype express only one of several possible surface proteins called immobilization antigens (i-antigens). A 16-kilobase plasmid…”
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Suppression of ColE1 high-copy-number mutants by mutations in the polA gene of Escherichia coli by YUN-LIANG YANG, POLISKY, B

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Conditional high copy number ColE1 mutants: resistance to RNA1 inhibition in vivo and in vitro by Fitzwater, T., Zhang, X. Y., Elble, R., Polisky, B.

“…We describe three independently isolated copy number mutants of a plasmid ColE1 derivative which undergo temperature‐ and growth‐phase‐dependent DNA…”
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Effect of altering GATC sequences in the plasmid ColE1 primer promoter by PATNAIK, P. K, MERLIN, S, POLISKY, B

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Permanent Rescue of a Non-Mendelian Mutation of Paramecium by Microinjection of Specific DNA Sequences by Jessop-Murray, H, Martin, L. D, Gilley, D, Preer-Jr., J. R, Polisky, B

“…The mutant Paramecium tetraurelia cell line d48 is unable to express the serotype A protein on its surface. Although the A gene is intact in the micronuclei of…”
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Mutants Affecting Processing of DNA in Macronuclear Development in Paramecium by Rudman, B, Preer, L. B, Polisky, B, Preer-Jr., J. R

“…In Paramecium tetraurelia, stock 51, the A surface protein is coded by the wild type A51 gene, present in micronuclei in two copies and in macronuclei in about…”
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Specificity of Substrate Recognition by the EcoRI Restriction Endonuclease by Polisky, Barry, Greene, Patricia, Garfin, David E., McCarthy, Brian J., Goodman, Howard M., Boyer, Herbert W.

“…The substrate specificity of the EcoRI restriction endonuclease can be varied in vitro by changing the pH and the ionic environment of the reaction…”
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Mutations affecting primer RNA interaction with the replication repressor RNA I in plasmid CoIE1: potential RNA folding pathway mutants by Polisky, B., Zhang, X.Y., Fitzwater, T.

“…The control of plasmid ColE1 copy number is mediated by the kinetics of interaction of two complementary plasmid‐encoded RNAs. One RNA is the primer precursor…”
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Multilevel regulation of surface antigen gene expression in Paramecium tetraurelia by GILLEY, D, RUDMAN, B. M, PREER, J. R, POLISKY, B

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