Effect of hesperetin on inflammatory and oxidative status in trinitrobenzene sulfonic acid-induced experimental colitis model

In our study, the effect of hesperetin on inflammatory and oxidative status in trinitrobenzene sulfonic acid (TNBS)-induced experimental colitis model was investigated through different methods. Eighteen Wistar albino male rats were divided in to three groups: Group I (Control, n = 8; 1 ml physiolog...

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Published in:	Cellular and Molecular Biology Vol. 64; no. 11; pp. 58 - 65
Main Authors:	Polat, Fatin Rustu, Karaboga, Ihsan, Polat, Muhammed Semih, Erboga, Zeynep, Yilmaz, Ahsen, Güzel, Savas
Format:	Journal Article
Language:	English
Published:	France 30-08-2018
Subjects:	Animals Antioxidants - metabolism Colitis - chemically induced Colitis - prevention & control Enzyme-Linked Immunosorbent Assay Hesperidin - therapeutic use Immunohistochemistry In Situ Nick-End Labeling Interleukin-6 - metabolism Lipid Peroxidation - drug effects Male Malondialdehyde - metabolism NF-kappa B - metabolism Nitric Oxide Synthase Type II - metabolism Oxidative Stress - drug effects Peroxidase - metabolism Rats Rats, Wistar Superoxide Dismutase - metabolism Trinitrobenzenesulfonic Acid - toxicity Tumor Necrosis Factor-alpha - metabolism iNOS TBNS-induced colitis Hesperetin NF-ĸβ MPO IL-6
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Summary:	In our study, the effect of hesperetin on inflammatory and oxidative status in trinitrobenzene sulfonic acid (TNBS)-induced experimental colitis model was investigated through different methods. Eighteen Wistar albino male rats were divided in to three groups: Group I (Control, n = 8; 1 ml physiological saline), Group II (Colitis, n = 8; 1 ml TNBS), Group III (Hesperetin, n = 8; 1 ml TNBS and 100 mg/kg hesperetin). Macroscopic and microscopic scores were calculated to determine the damage to the colon at the end of the experiment. Serum tumor necrosis factor-α (TNF-α) and tissue interleukin-6 (IL-6) levels were determined using the ELISA method. Myeloperoxidase (MPO), superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) levels were investigated spectrophotometrically. The TUNEL method was used for the detection of apoptotic cells in the colon tissue. Inducible nitric oxide synthase (iNOS) and nuclear factor-kappa-B (NF-ĸβ) expression in the colon were determined immunohistochemically. Hesperetin administration has shown to significantly reduce levels of MPO, MDA, and proinflammatory agents (TNF-α, IL-6, and NF-ĸβ). It has also been proven to inhibit mucosal apoptosis. This study indicates that hesperetin is protective against TNBS-induced colitis model via antiinflammatory, antioxidant and antiapoptotic effects.
ISSN:	0145-5680 1165-158X
DOI:	10.14715/cmb/2018.64.11.11