Adventitious root initiation in hypocotyl and epicotyl cuttings of eastern white pine (Pinus strobus) seedlings

The present paper reports results of experiments to develop a system for studying adventitious root initiation in cuttings derived from seedlings. Hypocotyl cuttings of 2‐week‐old eastern white pine (Pinus strobus L.) seedlings were treated for 5 min with 0, 100, 200, 300, 400, 500 or 600 mg l−1 (0,...

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Bibliographic Details
Published in:	Physiologia plantarum Vol. 102; no. 4; pp. 513 - 522
Main Authors:	Goldfarb, B. (North Carolina State Univ., Raleigh (USA)), Hackett, W.P, Furnier, G.R, Mohn, C.A, Plietzsh, A
Format:	Journal Article
Language:	English
Published:	Copenhagen Munksgaard International Publishers 01-04-1998 Blackwell
Subjects:	1-naphthaleneacetic acid ADVENTITIOUS ROOTS Agronomy. Soil science and plant productions auxin AUXINAS AUXINE AUXINS Biological and medical sciences BOUTURE CUTTINGS eastern white pine Economic plant physiology ENRACINEMENT ENRAIZAMIENTO EPICOTILOS epicotyl EPICOTYLE EPICOTYLS ESQUEJES ESTADO JUVENIL DE LAS PLANTAS Fundamental and applied biological sciences. Psychology Growth and development HIPOCOTILOS histology hypocotyl HYPOCOTYLE HYPOCOTYLS juvenility JUVENILITY OF PLANTS MADURACION MATURATION Morphogenesis, differentiation, rhizogenesis, tuberization. Senescence PINUS STROBUS Plant growth. Development of the storage organs Plant physiology and development PLANT TISSUES RACINE ADVENTIVE RAICES ADVENTICIAS ROOTING STADE JUVENILE DES PLANTES TEJIDOS VEGETALES TISSU VEGETAL Vegetative apparatus, growth and morphogenesis. Senescence Epicotyl Auxin 1-Naphthaleneacetic acid Plant juvenile growth stage Rhizogenesis Histology Adventitia Hypocotyl Pinus strobus Softwood forest tree Gymnospermae Plant growth substance Coniferales Spermatophyta Phytohormone
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Summary:	The present paper reports results of experiments to develop a system for studying adventitious root initiation in cuttings derived from seedlings. Hypocotyl cuttings of 2‐week‐old eastern white pine (Pinus strobus L.) seedlings were treated for 5 min with 0, 100, 200, 300, 400, 500 or 600 mg l−1 (0, 0.54, 1.07, 1.61, 2.15, 2.69 or 3.22 mM) 1‐naphthaleneacetic acid (NAA) to determine the effect on root initiation. The number of root primordia per cutting was correlated with NAA concentration and the square of NAA concentration. Thus, the number increased from less than one per cutting in the 0 NAA treatment to approximately 40 per cutting at 300 mg l‐1 NAA, above which no substantial further increase was observed. The larger number of root primordia formed in response to increasing concentrations of NAA was due to the formation of primordia over a larger proportion of the hypocotyls. Histological analysis of the timing of root primordium formation in hypocotyl cuttings revealed three discernible stages. Progression through these stages was relatively synchronous among NAA‐treated hypocotyl cuttings and within a given cutting, but variation was observed in the portion of different cuttings undergoing root formation. Control‐treated hypocotyl cuttings formed root primordia at lower frequencies and more slowly than NAA‐treated cuttings, with fewer primordia per cutting. Epicotyl cuttings from 11‐week‐old seedlings also formed adventitious roots, but more slowly than hypocotyl cuttings. NAA treatment of epicotyl cuttings caused more rapid root initiation and also affected the origin of adventitious roots in comparison with nontreated cuttings. NAA‐treated epicotyl cuttings formed roots in a manner analogous to that of the hypocotyl cuttings, directly from preformed vascular tissue, while control‐treated epicotyl cuttings first formed a wound or callus tissue and subsequently differentiated root primordia within that tissue. This system of inducing adventitious roots in pine stem cuttings lends itself to studying the molecular and biochemical steps that occur during root initiation and development.
Bibliography:	K01 F62 1998021822 istex:43FC468E9EF87BCF81673F8BBBFE550B79752B85 ArticleID:PPL1020405 ark:/67375/WNG-G9LT0NSM-S
ISSN:	0031-9317 1399-3054
DOI:	10.1034/j.1399-3054.1998.1020405.x