Adjusting the size of multicompartmental containers made of anionic liposomes and polycations by introducing branching and PEO moieties

Adjusting the size of multicompartmental containers made of anionic liposomes and polycations by introducing branching and PEO moieties

We propose a strategy to adjust the size of multiliposomal complexes made of small unilamellar anionic liposomes (lipid bilayer vesicles prepared from zwitterionic lipid 1,2-dioleoyl-sn-glycero-3-phosphocholine DOPC and ionic lipid 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-l-serine POPS with a typic...

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Bibliographic Details
Published in:Polymer (Guilford) Vol. 121; pp. 320 - 327
Main Authors: Pinguet, C.E., Hoffmann, J.M., Steinschulte, A.A., Sybachin, A., Rahimi, K., Wöll, D., Yaroslavov, A., Richtering, W., Plamper, F.A.
Format: Journal Article
Language:English
Published: Kidlington Elsevier Ltd 14-07-2017
Elsevier BV
Subjects:
Block copolymers
Cationic polymerization
Containers
Cryoforming
Electron microscopy
Electrostatics
Ethylene oxide
Fluorescence
L-Serine
Liposomes
Nanocontainers
Phosphocholine
Poly(ethylene oxide)
Polycations
Polyethylene oxide
Polymers
Self-assembly
Spectroscopy
Spectrum analysis
Star-shaped polycation
Studies
Transmission electron microscopy
Nanocontainers
Self-assembly
Poly(ethylene oxide)
Liposomes
Star-shaped polycation
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Summary:We propose a strategy to adjust the size of multiliposomal complexes made of small unilamellar anionic liposomes (lipid bilayer vesicles prepared from zwitterionic lipid 1,2-dioleoyl-sn-glycero-3-phosphocholine DOPC and ionic lipid 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-l-serine POPS with a typical diameter of 50 nm) with cationic polymers due to electrostatic attraction. Hereby, the use of quaternized poly(dimethylaminoethyl methacrylate), qPDMAEMA, allows the construction of multicompartmental carriers. Variation of the architecture and composition of various qPDMAEMA samples demonstrates the effect of introducing poly(ethylene oxide) PEO moieties and/or branching on the size of the hierarchical assemblies: both the PEO block (as seen for block copolymers and miktoarm stars) and the branching (as seen for star-shaped homopolymers and miktoarm stars) lead to smaller complexes compared to complexes with a linear qPDMAEMA homopolymer. Besides the use of cryogenic transmission electron microscopy (cryo-TEM), the formation of multicompartmental carriers composed of differently loaded liposomes is verified by two-color fluorescence cross-correlation spectroscopy (FCCS). [Display omitted] •Size of complexes among anionic liposomes and cationic polymers can be adjusted.•Use of copolymers with soluble/non-ionic chains decreases the size of complexes.•Use of branched polymers further decreases the size of complexes.•Joint transport of differently loaded liposomes is possible, as seen by FCCS.
ISSN:0032-3861
1873-2291
DOI:10.1016/j.polymer.2017.05.041